Elucidation of hydrogen sulfide-producing mechanism by oral bacteria and searching for novel prophylactic drugs that prevent oral malodor
| Project/Area Number |
21791810
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Iwate Medical University |
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Principal Investigator |
KEZUKA Yuichiro Iwate Medical University, 薬学部, 助教 (50397163)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 口腔生化学 / 酵素 / 細菌 / 薬学 / 硫化水素 / 立体構造 |
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| Research Abstract |
In this study, I focused on oral bacteria that highly produce hydrogen sulfide, a causative agent of oral malodor, and determined the structure of hydrogen sulfide-producing enzymes and their complex with a substrate analogue. We also succeeded to trap reaction intermediates. On the basis of structural information and/or enzymological data, conformational changes of enzyme during reaction and roles of amino acid residues at the active site were revealed. These results are quite important to descript their enzymatic reaction mechanisms and are expected to lead the search for novel prophylactic drugs that prevent oral malodor.
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Report
(3 results)
Research Products
(11 results)
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[Journal Article] Production of hydrogen sulfide by two enzymes associated with biosynthesis of homocysteine and lanthionine2010
Author(s)
Yoshida, Y., Ito S., Kamo, M., Kezuka, Y., Tamura, H., Kunimatsu, K., Kato, H.
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Journal Title
Fusobacterium nucleatum subsp. nucleatum ATCC 25586. Microbiology 156
Pages: 2260-2269
Related Report
Peer Reviewed
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