Analysis of molecular mechanism of inflammatory pain for pain control of pulpitis

• Project/Area Number: 21791849
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: NAGAYAMA Tomotaka Osaka University, 歯学部附属病院, 医員 (60456944)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 歯学 / シグナル伝達 / 神経科学 / 疼痛 / 炎症 / 歯髄炎 / 炎症性疼痛 / 後根神経節 / TRPV1 / CGRP / 免疫染色 / 初代培養 / レポーターアッセイ

Research Abstract

The regulation mechanism of CGRP expression and secretion induced by acid activation of TRPV1 signaling in DRG neurons, assuming local environment of inflammation, were investigated. Detailed analysis revealed that acid activation of TRPV1 lead to an up-regulation of CGRP expression via CREB-CaMK cascade.

Research Products

• [Journal Article] Involvement of acidic microenvironment in the pathophysiology of cancer-associated bone pain (2011)
  • Author(s): Toshiyuki Yoneda, Kenji Hata, Masako Nakanishi, Maho Nagae, Tomotaka Nagayama, Hiroki Wakabayashi, Toshihiko Nishisho, Teruhisa Sakurai, Toru Hiraga
  • Journal Title: Bone Volume: 48 Pages: 101-105
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Masako Nakanishi, Kenji Hata, Tomotaka Nagayama, Teruhisa Sakurai, Toshihiko Nishisho, Hiroki Wakabayashi, Toru Hiraga,_Shigeyuki Ebisu, Toshiyuki Yoneda.
  • Journal Title: Molecular Biology of the Cell Vol.21 Pages: 2568-2577
  • Peer Reviewed
• [Journal Article] Acid Activation of Trpv1 Leads to an Up-Regulation of Calcitonin Gene-related Peptide Expression in Dorsal Root Ganglion Neurons via the CaMK-CREB Cascade : A Potential Mechanism of Inflammatory Pain (2010)
  • Author(s): Masako Nakanishi, Kenji Hata, Tomotaka Nagayama, Teruhisa Sakurai, Toshihiko Nishisho, Hiroki Wakabayashi, Toru Hiraga, Shigeyuki Ebisu, Toshiyuki Yoneda
  • Journal Title: Molecular Biology of the Cell Volume: 21 Pages: 2568-2577
  • Peer Reviewed
• [Presentation] TRPV1-dependent CGRP expression regulates inflammatory pain (2010)
  • Author(s): Tomotaka Nagayama, Kenji Hata, Toshiyuki Yoneda, Shigeyuki Ebisu
  • Organizer: IADR 88^<th> General Session and Exibition
  • Place of Presentation: Barcelona (Spain)
  • Year and Date: 2010-07-15
• [Presentation] TRPV1-dependent CGRP expression regulates inflammatory pain. (2010)
  • Author(s): Tomotaka Nagayama, et. al.
  • Organizer: 88^<th> General Session and Exibition of the IADR
  • Place of Presentation: Barcelona, Spain.
  • Year and Date: 2010-06-15