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Analysis of the periodontal natural immunity activation mechanism by Beta-defensin-2 production

Research Project

Project/Area Number 21792137
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Periodontal dentistry
Research InstitutionOsaka Dental University

Principal Investigator

TAGUCHI Yoichiro  Osaka Dental University, 歯学部, 助教 (60434792)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsペプチド / 上皮細胞 / 炎症 / 歯周病治療 / 歯周病 / 歯肉 / 炎症性メディエーター
Research Abstract

Human β-defensin-2 (hBD-2) is one of antimicrobial peptides produced by epithelium cells after stimulation by microorganisms and inflammatory mediators. We reported that expression of hBD-2 mRNA in HGEC was P. gingivalis-dependently induced according to stimulation time and was connected with the initial stage of inflammatory responses after infection by pathogenic periodontal bacteria, with ultimately migration of neutrophils to infection sites. We investigated the difference between the expression of hBD-2 and the production of IL-8 in HGEC stimulated with various concentrations of P. gingivalis by RT-PCR and ELISA. Although the levels of hBD-2 mRNA and IL-8 production stimulated with P. gingivalis tended to increase with exposure time, the levels were not significantly changed by variations in concentration. These findings suggest that the expression of hBD-2 and IL-8 was observed in P. gingivalis-stimulated HGEC regardless of the severity of the inflammation.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (8 results)

All 2010 2009

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results) Book (2 results)

  • [Journal Article] ヒト歯肉上皮細胞に及ぼす影響から考えるエナメルマトリックスデリバティブ由来合成ペプチドの有用性2010

    • Author(s)
      田口洋一郎, 安井菜津希, 富永和也, 橋本典也, 至田宗泰, 玉置敏夫, 北條博一, 武田昭二, 林宏行, 田中昭男, 上田雅俊
    • Journal Title

      日本歯科保存学雑誌 53

      Pages: 449-456

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effectiveness of calcium hydroxide on LPS-induced hard tissue resorption.2010

    • Author(s)
      Muneyasu Shida, Tadahiko Baba, Hirotaka Kon'i, Yoichiro Taguchi, Masatoshi Ueda, Hiroyuki Hayashi.
    • Journal Title

      Journal of Osaka Dental University 44

      Pages: 145-150

    • NAID

      110007811664

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Double-immunostaining of p63 protein and cytokeratin 8 in pleomorphic adenoma.2009

    • Author(s)
      Masahiro Wato, Hirotaka Kon-i, Yoichiro Taguchi, Masatoshi Ueda, Ken-ichi Uobe, Kazuya Masuno, Kazuya Tominaga, Tetsunari Nishikawa, Akio Tanaka.
    • Journal Title

      Oral Medicine & Pathology 14(2)

      Pages: 49-54

    • NAID

      130004850536

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] In vitro effects of EMD-derived oligopeptides on human epithelial cells.2010

    • Author(s)
      Yasui N, Taguchi Y, Tominaga K, Hashimoto Y, Terada S, Takeda S, Tanaka A, Ueda M.
    • Organizer
      International Association for Dental Research 2010 Annual Meeting
    • Place of Presentation
      スペイン・バルセロナ
    • Year and Date
      2010-07-10
    • Related Report
      2010 Final Research Report
  • [Presentation] EMD由来合成ペプチドのヒト歯肉上皮細胞に及ぼす影響から考える至適濃度2010

    • Author(s)
      田口洋一郎, 安井菜津希, 富永和也, 田中昭男, 上田雅俊
    • Organizer
      日本歯科保存学会平成22年度春季学術大会
    • Place of Presentation
      熊本市
    • Year and Date
      2010-06-04
    • Related Report
      2010 Final Research Report
  • [Presentation] Effects of oligopeptide derived from enamel matrix derivative on rat bone marrow stromal cells2009

    • Author(s)
      Yoichiro Taguchi, Natsuki Yasui, Masatoshi Ueda et al.
    • Organizer
      American Academy of Periodontology 2009 Annual Meeting
    • Place of Presentation
      Boston, MA, USA
    • Related Report
      2009 Annual Research Report
  • [Book] 術審美再建歯周外科カラーアトラス第3版(第23章抜歯窩保存術第24章乳頭再建)(E.S.コーエン編)2009

    • Author(s)
      鴨井久一, 上田雅俊, 田口洋一郎
    • Publisher
      西村書店
    • Related Report
      2010 Final Research Report
  • [Book] コーエン審美歯周外科カラーアトラス-第3版-2009

    • Author(s)
      鴨井久一, 上田雅俊, 田口洋一郎, 他
    • Total Pages
      526
    • Publisher
      西村書店
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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