Analysis of the periodontal natural immunity activation mechanism by Beta-defensin-2 production

• Project/Area Number: 21792137
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Osaka Dental University
• Principal Investigator: TAGUCHI Yoichiro Osaka Dental University, 歯学部, 助教 (60434792)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ペプチド / 上皮細胞 / 炎症 / 歯周病治療 / 歯周病 / 歯肉 / 炎症性メディエーター

Research Abstract

Human β-defensin-2 (hBD-2) is one of antimicrobial peptides produced by epithelium cells after stimulation by microorganisms and inflammatory mediators. We reported that expression of hBD-2 mRNA in HGEC was P. gingivalis-dependently induced according to stimulation time and was connected with the initial stage of inflammatory responses after infection by pathogenic periodontal bacteria, with ultimately migration of neutrophils to infection sites. We investigated the difference between the expression of hBD-2 and the production of IL-8 in HGEC stimulated with various concentrations of P. gingivalis by RT-PCR and ELISA. Although the levels of hBD-2 mRNA and IL-8 production stimulated with P. gingivalis tended to increase with exposure time, the levels were not significantly changed by variations in concentration. These findings suggest that the expression of hBD-2 and IL-8 was observed in P. gingivalis-stimulated HGEC regardless of the severity of the inflammation.

Research Products

• [Journal Article] ヒト歯肉上皮細胞に及ぼす影響から考えるエナメルマトリックスデリバティブ由来合成ペプチドの有用性 (2010)
  • Author(s): 田口洋一郎, 安井菜津希, 富永和也, 橋本典也, 至田宗泰, 玉置敏夫, 北條博一, 武田昭二, 林宏行, 田中昭男, 上田雅俊
  • Journal Title: 日本歯科保存学雑誌 53 Pages: 449-456
  • Peer Reviewed
• [Journal Article] Effectiveness of calcium hydroxide on LPS-induced hard tissue resorption. (2010)
  • Author(s): Muneyasu Shida, Tadahiko Baba, Hirotaka Kon'i, Yoichiro Taguchi, Masatoshi Ueda, Hiroyuki Hayashi.
  • Journal Title: Journal of Osaka Dental University 44 Pages: 145-150
  • NAID: 110007811664
  • Peer Reviewed
• [Journal Article] Double-immunostaining of p63 protein and cytokeratin 8 in pleomorphic adenoma. (2009)
  • Author(s): Masahiro Wato, Hirotaka Kon-i, Yoichiro Taguchi, Masatoshi Ueda, Ken-ichi Uobe, Kazuya Masuno, Kazuya Tominaga, Tetsunari Nishikawa, Akio Tanaka.
  • Journal Title: Oral Medicine & Pathology 14(2) Pages: 49-54
  • NAID: 130004850536
  • Peer Reviewed
• [Presentation] In vitro effects of EMD-derived oligopeptides on human epithelial cells. (2010)
  • Author(s): Yasui N, Taguchi Y, Tominaga K, Hashimoto Y, Terada S, Takeda S, Tanaka A, Ueda M.
  • Organizer: International Association for Dental Research 2010 Annual Meeting
  • Place of Presentation: スペイン・バルセロナ
  • Year and Date: 2010-07-10
• [Presentation] EMD由来合成ペプチドのヒト歯肉上皮細胞に及ぼす影響から考える至適濃度 (2010)
  • Author(s): 田口洋一郎, 安井菜津希, 富永和也, 田中昭男, 上田雅俊
  • Organizer: 日本歯科保存学会平成22年度春季学術大会
  • Place of Presentation: 熊本市
  • Year and Date: 2010-06-04
• [Presentation] Effects of oligopeptide derived from enamel matrix derivative on rat bone marrow stromal cells (2009)
  • Author(s): Yoichiro Taguchi, Natsuki Yasui, Masatoshi Ueda et al.
  • Organizer: American Academy of Periodontology 2009 Annual Meeting
  • Place of Presentation: Boston, MA, USA
• [Book] 術審美再建歯周外科カラーアトラス第3版(第23章抜歯窩保存術第24章乳頭再建)(E.S.コーエン編) (2009)
  • Author(s): 鴨井久一, 上田雅俊, 田口洋一郎
  • Publisher: 西村書店
• [Book] コーエン審美歯周外科カラーアトラス-第3版- (2009)
  • Author(s): 鴨井久一, 上田雅俊, 田口洋一郎, 他
  • Total Pages: 526
  • Publisher: 西村書店