Effects of Hydrogen Sulphide on Osteoclast Differentiation in Rats

• Project/Area Number: 21792149
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Social dentistry
• Research Institution: Okayama University
• Principal Investigator: IRIE Kouichiro Okayama University, 岡山大学病院, 医員 (50509594)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 口臭 / 揮発性化合物 / ラット / 口臭起因物質 / LPS / 歯周組織

Research Abstract

Oral malodor is mainly attributed to volatile sulphur compounds (VSCs) such as hydrogen sulphide (H_2S). VSC accelerate periodontal soft tissue destruction. However, there is little information about the potential role of H_2S in alveolar bone loss. The purpose of this animal study was to examine the effects ofH2S on osteoclast differentiation in rat periodontal tissue.H2S application caused a transient increase of osteoclast differentiation with up-regulation of RANKL expression in osteoblasts. H_2S, which is primarily responsible for halitosis, may also contribute to alveolar bone resorption through RANKL expression. H_2S and LPS had the additive effects on osteoclast differentiation through activation of TLR4 pathway.

Research Products

• [Journal Article] Occlusal disharmony induces BDNF level in rat submandibular gland. (2011)
  • Author(s): Irie K, Ekuni D, Tomofuji T, Azuma T, Endo Y, Kasuyama K, Morita M
  • Journal Title: Arch Oral Biol 56 Pages: 35-40
  • NAID: 120003367828
  • Peer Reviewed
• [Journal Article] Experimental periodontitis induces gene expression of proinflammatory cytokines in liver and white adipose tissues in obesity. (2010)
  • Author(s): Endo Y, Tomofuji T, Ekuni D, Irie K, Azuma T, Tamaki N, Yamamoto T, Morita M
  • Journal Title: J Periodontol 81 Pages: 520-526
  • Peer Reviewed
• [Journal Article] Effects of periodontitis on aortic insulin resintance in an obese rat model. (2010)
  • Author(s): Ekuni D, Tomofuji T, Irie K, Kasuyama K, Umakosi M, Azuma T, Tamaki N, Sanbe T, Endo Y, Yamamoto T
  • Journal Title: Lab Invest 90 Pages: 348-359
  • Peer Reviewed
• [Journal Article] Imbalanc of oxidative/anti-oxidative status induced by periodontitis is involved in apoptosis of rat submandubular glands. (2010)
  • Author(s): Ekuni D, Endo Y, Irie K, Azuma T, Tamaki N, Tomofuji T, Morita M
  • Journal Title: Arch Oral Biol 55 Pages: 170-176
  • Peer Reviewed
• [Journal Article] Relationship between periodontitis and hepatic abnormalities in youg adults. (2010)
  • Author(s): Furuta M, Ekuni D, Yamamoto T, Irie K, Koyama R, Sanbe T, Yamanaka R, Morita M, Kuroki K, Tobe K.
  • Journal Title: Acta Odontol Scand 68 Pages: 27-33
  • Peer Reviewed
• [Journal Article] A single application of hydrogen sulphide induces s transient osteoclast differentiation with RANKL expression in the rat model (2009)
  • Author(s): Irie K, Ekuni D, Yamamoto T, Morita M, Yaegaki K, Ii H, Imai T
  • Journal Title: Arch Oral Biol 54 Pages: 723-729
  • Peer Reviewed
• [Journal Article] Preventive effects of a cocoa-enriched diet on gingival oxidative stress in experimental periodontitis. (2009)
  • Author(s): Tomofuji T, Ekuni D, Irie K, Azuma T, Endo Y, Tamaki N, Sanbe T, Murakami J, Yamamoto T, Morita M
  • Journal Title: J Periodontol 80 Pages: 1799-1808
  • Peer Reviewed
• [Journal Article] A single application of hydrogen sulphide induces a transient osteoclast differentiation with RANKL expression in the rat model (2009)
  • Author(s): Irie K
  • Journal Title: Archives of oral biology 54 Pages: 723729-723729
  • Peer Reviewed
• [Presentation] Combined Effects of Hydrogen Sulphide and Lipopolysaccharide in Rat Periodontium (2011)
  • Author(s): Irie.K
  • Organizer: 89th International Association for Dental Research
  • Place of Presentation: San Diego
  • Year and Date: 2011-03-19
• [Presentation] Combined Effects of Hydrogen Sulphide and Lipopolysaccharide in Rat Periodontium. (2011)
  • Author(s): K.IRIE, D.EKUNI, T.TOMOFUJI, Y.ENDO, K.KASUYAMA, M.MORITA, K.YAEGAKI, H.II, T.IMAI
  • Organizer: The 89th International Association for Dental Research
  • Place of Presentation: Barcelona
• [Presentation] Combined Effects of Hydrogen Sulphide and Lipopolysaccharide in Rat Periodontium (2010)
  • Author(s): Irie.K
  • Organizer: 9th INTERNATIONAL CONFERENCE OF ASIAN ACADEMY OF PREVENTIVE DENTISTRY
  • Place of Presentation: Kuala Lumpur
  • Year and Date: 2010-11-10
• [Presentation] Combined Effects of Hydrogen Sulphide and Lipopolysaccharide in Rat Periodontium. (2010)
  • Author(s): K.IRIE, D.EKUNI, T.TOMOFUJI, Y.ENDO, K.KASUYAMA, M.MORITA, K.YAEGAKI, H.II, T.IMAI
  • Organizer: The 9th International Conference of Asian Academy of Preventive Dentistry
  • Place of Presentation: Kuala Lumpur
• [Presentation] Cocoa-enriched diet decreases (2009)
  • Author(s): Irie K, Tomofuji T, Ekuni D, Azuma T, Tamaki N, Endo Y, Sanbe T, Yamamoto TMorita M
  • Organizer: gingival oxidative stress in rat periodontitis: SFRR
  • Place of Presentation: Roma, Italy
• [Presentation] Hydrogen Sulfide Application Induces a Transient Osteoclast Differentiation with RANKL Expression in the Rat Model (2009)
  • Author(s): Ekuni D, Irie K, Yamamoto T, Morita M, Yaegaki K, Ii H, Imai T
  • Organizer: The 8th congress of International Society for Breath Odor Research
  • Place of Presentation: Dortmund