• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

In vivo chronic imaging of neuronal circuit plasticity using a genetically-encoded calcium sensor

Research Project

Project/Area Number 21800091
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SATO Masaaki  The Institute of Physical and Chemical Research, シナプス機能研究チーム, 研究員 (90518325)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2010: ¥1,625,000 (Direct Cost: ¥1,250,000、Indirect Cost: ¥375,000)
Fiscal Year 2009: ¥1,755,000 (Direct Cost: ¥1,350,000、Indirect Cost: ¥405,000)
Keywords蛍光カルシウムバイオセンサー / ウイルスベクター / トランスジェニックマウス / 子宮内電気穿孔法 / 二光子レーザー顕微鏡 / 記憶・学習 / 可塑性 / 二光子レーザ顕微鏡 / 神経可塑性
Research Abstract

To establish chronic in vivo two-photon calcium imaging, we generated transgenic mice that stably express a genetically-encoded calcium sensor GCaMP4 in layer 5 of cortex and CA1 of hippocampus. Our in vivo two-photon imaging confirmed that the fluorescence of GCaMP4 in hippocampal CA1 pyramidal cells of these mice increased rapidly and substantially in response to pharmacologically-induced elevation of neuronal circuit activity. These transgenic mice will thus become a valuable tool for longitudinal imaging studies of learning-induced neuronal circuit plasticity in the brain.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (5 results)

All 2010 Other

All Presentation (4 results) Remarks (1 results)

  • [Presentation] In vivo two-photon imaging of hippocampal CA1 neuronal circuits in mice2010

    • Author(s)
      佐藤正晃
    • Organizer
      The 40^<th> annual meeting of the Society for Neuroscience
    • Place of Presentation
      米国カリフォルニア州サンディェゴ市
    • Year and Date
      2010-11-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス海馬神経回路の構造と機能のin vivo二光子イメージング2010

    • Author(s)
      佐藤正晃
    • Organizer
      Neuro2010(第33回日本神経科学大会・第53回日本神経科学会大会・第20回日本神経回路学会大会)
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2010-09-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウス海馬神経回路の構造と機能のin vivo二光子イメージングNeuro20102010

    • Author(s)
      佐藤正晃、河野真子、中井淳一、林康紀
    • Organizer
      第33回日本神経科学大会、第53回日本神経化学会大会、第20回日本神経回路学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] In vivo two-photon imaging of hippocampal CA1 neuronal circuits in mice.2010

    • Author(s)
      Masaaki Sato, Masako Kawano, Junichi Nakai, Yasunori Hayashi
    • Organizer
      The 40th annual meeting of the Society for Neuroscience
    • Place of Presentation
      San Diego, CA, USA
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://glutamate.brain.riken.jp/dokuwiki/doku.php?id=ja:masaaki_sato

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi