Development of fluorescent strain sensor and imaging system

• Project/Area Number: 21870004
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Hirosaki University
• Principal Investigator: IWAI Sosuke Hirosaki University, 教育学部, 講師 (40366547)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,704,000 (Direct Cost: ¥2,080,000、Indirect Cost: ¥624,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
• Keywords: GFP / ストレインセンサー / スペクトル線形分離法 / スペクトル分離

Research Abstract

To detect external force-induced conformational changes of proteins in living cells, I improved and evaluated the GFP-based fluorescent strain sensor. Also, toward the establishment of an imaging system suitable for the sensor, I theoretically estimated the maximum errors in the ratio values of ratiometric sensors, obtained by linear spectral unmixing, which is based on the least squares regression method. The estimation would provide an useful criterion to validate the results obtained by spectral imaging of ratiometric sensors.

Research Products

• [Journal Article] スペクトル線形分離法における誤差を考える (2010)
  • Author(s): 岩井草介
  • Journal Title: バイオイメージング Vol.19、No.2 Pages: 1-6
  • NAID: 10027808946
• [Journal Article] Myosin-actin interaction in Dictyostelium cells revealed by GFP-based strain sensor and validated linear spectral unmixing. (2010)
  • Author(s): Sosuke Iwai, Taro Q. P.Uyeda
  • Journal Title: Cytometry A Vol.77A, No.8 Pages: 743-750
  • Peer Reviewed
• [Journal Article] GFPを用いた「ストレインセンサー」でミオシン-アクチン相互作用を可視化する (2010)
  • Author(s): 岩井草介
  • Journal Title: 生物物理 Vol.50, No.5 Pages: 238-239
• [Journal Article] Photoregulated Assembly/Disassembly of DNA-templated Protein Arrays Using Modified Oligonucleotide Carrying Azobenzene Side Chains. (2010)
  • Author(s): You Hachikubo, Sosuke Iwai, Taro Q.P.Uyeda
  • Journal Title: Biotechnology and Bioengineering Vol.106, No.1 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Thermal activation energy for bidirectional movement of actin along bipolar tracks of myosin filaments. (2010)
  • Author(s): Hiroyuki Okubo, Masanori Iwai, Sosuke Iwai, Shigeru Chaen
  • Journal Title: Biochemical and Biophysical Research Communications Vol.396, No.2 Pages: 539-542
  • Peer Reviewed
• [Journal Article] スペクトル線形分離法における誤差を考える (2010)
  • Author(s): 岩井草介
  • Journal Title: バイオイメージング Volume: 19 Pages: 1-6
  • NAID: 10027808946
• [Journal Article] Myosin-actin interaction in Dictyostelium cells revealed by GFP-based strain sensor and validated linear spectral unmixing. (2010)
  • Author(s): Sosuke Iwai, Taro Q.P.Uyeda
  • Journal Title: Cytometry A Volume: 77A Pages: 743-750
  • Peer Reviewed
• [Presentation] ミオシンフィラメント双極トラックに沿ったアクチンフィラメント滑り運動の活性化エネルギー (2010)
  • Author(s): Hiroyuki Okubo, Masanori Iwai, Sosuke Iwai, Shigeru Chaen
  • Organizer: 日本生物物理学会
  • Place of Presentation: 東北大学
  • Year and Date: 2010-09-20
• [Presentation] スペクトル線形分離法における誤差の検討 (2010)
  • Author(s): 岩井草介
  • Organizer: 日本バイオイメージング学会
  • Place of Presentation: 慶応義塾大学
  • Year and Date: 2010-09-10
• [Presentation] 誤差を含む混合蛍光スペクトルに対するスペクトル分離の有効性の検討 (2009)
  • Author(s): 岩井草介
  • Organizer: 日本生物物理学会
  • Place of Presentation: アスティとくしま
  • Year and Date: 2009-11-01
• [Presentation] 誤差を含む混合蛍光スペクトルに対するスペクトル分離の有効性の検討 (2009)
  • Author(s): 岩井草介、上田太郎
  • Organizer: 日本生物物理学会年会
  • Place of Presentation: アスティ徳島
  • Year and Date: 2009-11-01