Regulation of myosin II phosphorylation and dephosphorylation during apoptosis

• Project/Area Number: 21870029
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Ehime University
• Principal Investigator: IWASAKI Takahiro Ehime University, ベンチャー・ビジネス・ラボラトリー, 研究員 (40542999)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000); Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000); Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
• Keywords: アポトーシス / ミオシン / 脱リン酸化 / カスパーゼ / ミオシンホスファターゼ / カスパーゼ3 / メンブレンブレッビング / ミオシン脱リン酸化

Research Abstract

It is known that myosin II is hyperphosphorylated in apoptotic cells. However, the regulation of myosin phosphatase (MP) in apoptosis is still unclear. In this study, we demonstrated that the activity of MP is reduced by caspae-3 mediated cleavage of myosin binding subunit. This result suggests that hyperphosphorylation of myosin II is induced by activation of myosin kinase and inactivation of MP during apoptosis.

Research Products

• [Journal Article] Production and partial purification of membrane proteins using a liposome-supplemented wheat cell-free translation system. (2011)
  • Author(s): A.Nozawa, T.Ogasawara, S.Matsunaga, T.Iwasaki, T.Sawasaki, Y.Endo
  • Journal Title: BMC Biotechnology 11 Pages: 35-35
  • Peer Reviewed
• [Journal Article] Caspase-8 cleavage of the interleukin-21 (IL-21) receptor is a negative feedback regulator of IL-21 signaling. (2011)
  • Author(s): T.Akagi, K.Shimizu, S.Takahama, T.Iwasaki, K.Sakamaki, Y.Endo, T.Sawasaki
  • Journal Title: FEBS letters 585 Pages: 1835-1840
  • Peer Reviewed
• [Presentation] Regulation of myosin phosphatase during apoptosis (2010)
  • Author(s): 岩崎隆宏、遠藤弥重太、澤崎達也
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Regulation of myosin phosphatase during apoptosis (2010)
  • Author(s): 岩崎隆宏
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2010-12-08
• [Presentation] Downregulation of myosin phosphatase targeting subunit 1 during apoptosis. (2010)
  • Author(s): 岩崎隆宏, 澤崎達也, 遠藤弥重太
  • Organizer: The 8th Matsuyama International Symposium on Cell-Free Sciences
  • Place of Presentation: 松山
  • Year and Date: 2010-09-28
• [Presentation] アポトーシス時におけるミオシンホスファターゼ活性調節機構 (2010)
  • Author(s): 岩崎隆宏、遠藤弥重太、澤崎達也
  • Organizer: 第62回日本細胞生物学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-19
• [Presentation] アポトーシス時におけるミオシンホスファターゼ活性調節機構 (2010)
  • Author(s): 岩崎隆宏
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-05-19
• [Presentation] Role of myosin phosphatase cleavage by caspase-3 during apoptosis. (2009)
  • Author(s): 岩崎隆宏, 澤崎達也, 遠藤弥重太
  • Organizer: American Society for Cell Biology 46th annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-12-06
• [Presentation] Role of myosin phosphatase cleavage by caspase-3 during apoptosis (2009)
  • Author(s): 岩崎隆宏
  • Organizer: American Society for Cell biology 46th annual meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2009-12-06
• [Presentation] Regulation of membrane blebbing during apoptosis by caspase cleavage of myosin phosphatase. (2009)
  • Author(s): 岩崎隆宏, 澤崎達也, 遠藤弥重太
  • Organizer: The 7th Matsuyama International Symposium on Cell-Free Sciences
  • Place of Presentation: 松山
  • Year and Date: 2009-09-24