Analysis of the activation mechanism of FilGAP by phosphorylation in establishment and maintenance of cell polarity.
Project/Area Number |
21870031
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Functional biochemistry
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Research Institution | Kitasato University |
Principal Investigator |
NAKAZAWA Yuki Kitasato University, 理学部, 助教 (50508851)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
|
Keywords | 細胞極性 / リン酸化 / シグナル伝達 |
Research Abstract |
To understand the activation mechanism of the Rac GAP FilGAP, several FilGAP-binding proteins were isolated and identified by mass-spectrometry. Theanalysis demonstrated that FilGAP forms complexes with several RNA binding proteins. Oneof them, RBM10 was found to bind to FilGAP at cell edges and its tyrosine phosphorylationby Src-family enhances their binding. It appeared that RBM10 regulates expression ofFilGAP. In addition, analysis using an antibody against phosphorylated(activated) FilGAPdemonstrated the activation level of FilGAP during cell spreading.
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Report
(3 results)
Research Products
(1 results)