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Serological characterizations of tandem repeat proteins for detection of African trypanosome infection

Research Project

Project/Area Number 21880007
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionThe University of Tokyo (2010)
Obihiro University of Agriculture and Veterinary Medicine (2009)

Principal Investigator

GOTO Yasuyuki  The University of Tokyo, 大学院・農学生命科学研究科, 助教 (50553434)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,626,000 (Direct Cost: ¥2,020,000、Indirect Cost: ¥606,000)
Fiscal Year 2010: ¥1,131,000 (Direct Cost: ¥870,000、Indirect Cost: ¥261,000)
Fiscal Year 2009: ¥1,495,000 (Direct Cost: ¥1,150,000、Indirect Cost: ¥345,000)
Keywordsトリパノソーマ症 / 血清学的診断 / 抗原 / タンデムリピート
Research Abstract

Serological diagnosis is a useful method to detect African trypanosome infection. Currently available serological tests utilize whole parasites or crude antigens. Recombinant antigens may not only enhance test specificity by allowing the detection of species-specific antibodies, but could also improve reproducibility/standardization and reduce production costs. With a goal of identifying such recombinant proteins, we computationally identified proteins with tandem repeat (TR) domain from the parasite proteomes, expressed them and evaluated their potential for serological diagnosis of African trypanosome infections in cattle. Among those tested, Tbg4 demonstrated the best performance with 89% sensitivity and 100% specificity. Our data indicate the potential of these defined antigens for detection of animal African trypanosomiasis.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2011 2010 2009

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] Biased cellular locations of tandem repeat antigens in African trypanosomes.2011

    • Author(s)
      Goto Y, Duthie MS, Kawazu SI, Inoue N, Carter D.
    • Journal Title

      Biochem Biophys Res Commun 405

      Pages: 434-438

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biased cellular locations of tandem repeat antigens in African trypanosomes2011

    • Author(s)
      Goto Y, et. al.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 405 Pages: 434-438

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Commonness and uniqueness of tandem repeat antigens in the trypanosomatid parasites.2011

    • Author(s)
      Goto Y, Carter D, Duthie MS, Matsumoto Y, Kawazu SI, Inoue N, Reed SG.
    • Organizer
      The 45th Annual Japan-U.S.Joint Conference on Parasitic Diseases.
    • Place of Presentation
      国立感染症研究所
    • Year and Date
      2011-01-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Commonness and uniqueness of tandem repeat antigens in the trypanosomatia parasites2011

    • Author(s)
      Goto Y
    • Organizer
      The 45th Annual Japan-U.S. Joint Conference on Parasitic Diseases
    • Place of Presentation
      国立感染症研究所(東京)
    • Year and Date
      2011-01-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 原虫におけるタンデムリピート抗原の意義2010

    • Author(s)
      後藤康之、Darrick Carter、井上昇、河津信一郎、Steven G.Reed
    • Organizer
      第79回日本寄生虫学会大会
    • Place of Presentation
      旭川市大雪クリスタルホール
    • Year and Date
      2010-05-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 原虫におけるタンデムリピート抗原の意義2010

    • Author(s)
      後藤康之
    • Organizer
      第79回日本寄生虫学会大会
    • Place of Presentation
      旭川市大雪クリスタルホール(旭川)
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] タンデムリピート探索による原虫症血清学的診断抗原の同定2009

    • Author(s)
      後藤康之
    • Organizer
      日本熱帯医学会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県)
    • Year and Date
      2009-10-22
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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