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Structural analysis and protein engineering for clarifying the molecular mechanism of the taste-modifying activity of neoculin

Research Project

Project/Area Number 21880015
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionThe University of Tokyo

Principal Investigator

NAKAJIMA Kenichiro  The University of Tokyo, 大学院・農学生命科学研究科, 特任助教 (70554492)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Keywords味覚修飾活性 / ヒスチジン / NMR / 甘味タンパク質 / pH依存性
Research Abstract

Neoculin is a sweet protein with taste-modifying activity converting sourness into sweetness. In this study, I performed a mutational analysis of neoculin to identify the important residue(s) for this pH-dependent activity. It revealed that one histidine residue in neoculin was critical for the activity. NMR analysis also performed to evaluate the structural-activity relationship of neoculin. It was found that the neoculin structurally changed between neutral and acidic pH.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (12 results)

All 2011 2010 2009

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (6 results) Book (1 results)

  • [Journal Article] Identification and Modulation of the Key Amino Acid Residue Responsible for the pH Sensitivity of Neoculin, a Taste-modifying Protein2011

    • Author(s)
      Ken-ichiro Nakajima, Kanako Yokoyama, Taichi Koizumi, Ayako Koizumi, Tomiko Asakura, Tohru Terada, Katsuyoshi Masuda, Keisuke Ito, Akiko Shimizu-Ibuka, Takumi Misaka, Keiko Abe
    • Journal Title

      PLoS One under review

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-acidic Compounds Induce the Intense Sweet Taste of Neoculin, a Taste-modifying Protein2011

    • Author(s)
      Ken-ichiro Nakajima, Ayako Koizumi, Kisho Iizuka, Keisuke Ito, Yuji Morita, Taichi Koizumi, Tomiko Asakura, Akiko Shimizu-Ibuka, Takumi Misaka, Keiko Abe
    • Journal Title

      Biosci.Biotechnol.Biochem. under review

    • NAID

      10029591898

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification and Modulation of the Key Amino Acid Residue Responsible for the pH Sensitivity of Neoculin, a Taste-modifying Protein2011

    • Author(s)
      Ken-ichiro Nakajima, et. al.
    • Journal Title

      PLoS One

      Volume: 印刷中(掲載確定)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-acidic Compounds Induce the Intense Sweet Taste of Neoculin, a Taste-modifying Protein2011

    • Author(s)
      Ken-ichiro Nakajima, et. al.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 印刷中(掲載確定)

    • NAID

      10029591898

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] pH-dependent Structural Change of Neoculin in Special Reference to Its Taste-modifying Activity2009

    • Author(s)
      Morita Y, Nakajima K, Iizuka K, Terada T, Shimizu-Ibuka A, Ito K, Koizumi A, Asakura T, Misaka T, Abe K.
    • Journal Title

      Biosci.Biotechonol.Biochem. 73

      Pages: 2552-2555

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] NMRによる味覚修飾タンパク質ネオクリンの構造変化の解析2011

    • Author(s)
      小泉太一、寺田透、中島健一朗、古泉文子、土屋麻美、朝倉富子、阿部啓子、三坂巧
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 点変異導入による味覚修飾タンパク質ネオクリンの活性制御2011

    • Author(s)
      金田康平、中島健一朗、小泉太一、古泉文子、土屋麻美、朝倉富子、阿部啓子、三坂巧
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 味覚修飾タンパク質ネオクリンの構造、機能相関の分光学的解析2010

    • Author(s)
      小泉太一、森田悠治、中島健一朗、飯塚希翔、古泉文子、清水(井深)章子、朝倉富子、三坂巧、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 味覚修飾タンパク質ネオクリンとそのバリアントの培養細胞を用いた活性評価2010

    • Author(s)
      中島健一朗、横山可那子、清水(井深)章子、小泉太一、古泉文子、朝倉富子、村清司、徳江千代子、荒井綜一、三坂巧、阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 味覚修飾タンパク質ネオクリンとそのバリアントの培養細胞を用いた活性評価2010

    • Author(s)
      中島健一朗, 横山可那子, 清水(井深)章子, 小泉太一, 古泉文子, 朝倉富子, 村清司, 徳江千代子, 荒井綜一, 三坂巧, 阿部啓子
    • Organizer
      日本農芸化学会
    • Place of Presentation
      東京(東京大学)
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] Analyzing the structure-function relationship in neoculin, a sweet protein with taste-modifying activity.2009

    • Author(s)
      中島健一朗, Koizumi A, 朝倉富子, 三坂巧, 阿部啓子
    • Organizer
      The 7th International Symposium on Molecular and Neural Mechanisms of Taste and Olfactory Perception
    • Place of Presentation
      福岡(九州大学)
    • Year and Date
      2009-11-03
    • Related Report
      2009 Annual Research Report
  • [Book] 蛋白質 核酸 酵素2009

    • Author(s)
      中島健一朗, 古泉文子, 朝倉富子, 三坂巧
    • Total Pages
      100
    • Publisher
      共立出版
    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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