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Functional characterization of iridoid glycoside and biosynthetic enzymes in plant chemical defense

Research Project

Project/Area Number 21880021
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionShizuoka University

Principal Investigator

OHNISHI Toshiyuki  Shizuoka University, 若手グローバル研究リーダー育成拠点, 特任助教(テニュアトラック) (60542165)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,756,000 (Direct Cost: ¥2,120,000、Indirect Cost: ¥636,000)
Fiscal Year 2010: ¥1,313,000 (Direct Cost: ¥1,010,000、Indirect Cost: ¥303,000)
Fiscal Year 2009: ¥1,443,000 (Direct Cost: ¥1,110,000、Indirect Cost: ¥333,000)
Keywords植物 / 酵素 / 昆虫 / 農林水産物 / 生理活性
Research Abstract

Oleuropein, a secoiridoid glycoside, found in several Oleaceae species such as the olive tree and privet tree. The activated iridoid glycoside has adverse effects against herbivores by decreasing the nutritive value of plant protein. Nevertheless the activation pathway has not been clearly proven yet. To investigate the physiological function of iridoid glycoside in chemical defense, we purified a β-GH from olive fruit and characterized the enzymatic activity of an olive β-GH and cloning the cDNA of olive β-GH

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (28 results)

All 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (23 results) Remarks (3 results)

  • [Journal Article] Insights into the function and evolution of P450s in plant steroid metabolism.2009

    • Author(s)
      Ohnishi, T., Yokota, T., Mizutani, M.
    • Journal Title

      Phytochemistry vol.70, No.17-18

      Pages: 1918-1929

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Insights into the function and evolution of P450s in plant steroid metabolism2009

    • Author(s)
      大西利幸
    • Journal Title

      Phytochemistry 70

      Pages: 1918-1929

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] オリーブ化学防御物質オレウロペインの活性化経路の解明2011

    • Author(s)
      松野聡、渡辺修治、大西利幸
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] イリドイド配糖体糖加水分解酵素の酵素学的研究2011

    • Author(s)
      大上将司, 松野聡, 平田拓, 道羅英夫, 渡辺修治, 大西利幸
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物の化学防御機構におけるイリドイド配糖体糖加水分解酵素の生化学的研究2011

    • Author(s)
      大上将司
    • Organizer
      第45回植物化学調節学会
    • Place of Presentation
      神戸大学六甲キャンパス(兵庫県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] オリーブ化学防御物質オレウロペインの活性化経路の解明2011

    • Author(s)
      松野聡
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都府)
    • Related Report
      2010 Annual Research Report
  • [Presentation] イリドイド配糖体糖加水分解酵素の酵素学的研究2011

    • Author(s)
      大上将司
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都府)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Quest for floral aroma in fresh tea leaves (Camellia sinensis)2010

    • Author(s)
      大西利幸
    • Organizer
      Seminar of the Departmental Meeting series autumn/winter 2010/2011
    • Place of Presentation
      Denmark
    • Year and Date
      2010-09-02
    • Related Report
      2010 Final Research Report
  • [Presentation] Quest for floral aroma in fresh tea leaves (Camellia sinensis)2010

    • Author(s)
      Toshiyuki Ohnishi
    • Organizer
      Seminar of the Departmental Meeting series autumn/winter 2010/2011
    • Place of Presentation
      CopenhagenUniversity(Denmark)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Biochemical characterization of monoterpene UDP-glcosidase in Camellia sinensis2009

    • Author(s)
      大西利幸
    • Organizer
      Germany-Japan Joint Symposium and Graduate Students Forum for the Promotion of the DDP
    • Place of Presentation
      Shizuoka
    • Year and Date
      2009-11-25
    • Related Report
      2010 Final Research Report
  • [Presentation] Biochemical characterization of monoterpene UDP-glcosidase in Camellia sinensis2009

    • Author(s)
      大西利幸
    • Organizer
      Germany-Japan Joint Symposium and Graduate StudentsForum for the Promotion of the DDP
    • Place of Presentation
      静岡市産学交流センター
    • Year and Date
      2009-11-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] シトクロムP450酵素がもたらす植物生理活性物質の多様性-P450研究の現状と今後の展開2009

    • Author(s)
      大西利幸
    • Organizer
      メタボローム・代謝ネットワーク勉強会
    • Place of Presentation
      横浜
    • Year and Date
      2009-07-27
    • Related Report
      2010 Final Research Report
  • [Presentation] シトクロムP450酵素がもたらす植物生理活性物質の多様性-P450研究の現状と今後の展開2009

    • Author(s)
      大西利幸
    • Organizer
      メタボローム・代謝ネットワーク勉強会
    • Place of Presentation
      理化学研究所
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 針葉樹の防御機構におけるジテルペン樹脂酸生合成酵素の役割の解明2009

    • Author(s)
      大西利幸
    • Organizer
      日本農芸化学会中部支部第155回例会
    • Place of Presentation
      静岡
    • Year and Date
      2009-07-18
    • Related Report
      2010 Final Research Report
  • [Presentation] 針葉樹の防御機構におけるジテルペン樹脂酸生合成酵素の役割の解明2009

    • Author(s)
      大西利幸
    • Organizer
      日本農芸化学会中部支部第155回例会
    • Place of Presentation
      静岡市産学交流センター
    • Year and Date
      2009-07-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] 茶の香気前駆体である配糖体生合成に関与する糖転移酵素の精製2009

    • Author(s)
      大西利幸、谷田彩、渡辺修治
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] オリーブにおけるオレウロペイン内生量の部位別・時期別変化および傷害応答による変動2009

    • Author(s)
      松野聡、渡辺修治、大西利幸
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] 植物の防御機構におけるイリドイド配糖体糖加水分解酵素の探索2009

    • Author(s)
      大西利幸、松野聡、渡辺修治
    • Organizer
      第44回植物化学調節学会
    • Place of Presentation
      仙台
    • Related Report
      2010 Final Research Report
  • [Presentation] Biochemical characterization of CYP720B subfamily members involved in diterpene resin acid biosynthesis in Sitka Spruce2009

    • Author(s)
      Ohnishi T., Hamberger B., Hamberger B., Bohlman, J.
    • Organizer
      9th International Meeting : Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites.
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] Gene stracture and expression of the cythchrome P450 CTP720B family in Stika spruce (Picea sitchensis)2009

    • Author(s)
      Hamberger B., Ohnishi T., Hamberger B., Bohlman, J.
    • Organizer
      9th International Meeting : Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites.
    • Place of Presentation
      東京
    • Related Report
      2010 Final Research Report
  • [Presentation] Biochemical characterization of CYP720B subfamily members involved in diterpene resin acid biosynthesis in Sitka Spruce2009

    • Author(s)
      Ohnishi, T., Hamberger, B., Hamberger, B., Bohlman
    • Organizer
      9th International Meeting : Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites
    • Place of Presentation
      東京大学弥生講堂
    • Related Report
      2009 Annual Research Report
  • [Presentation] Gene stracture and expression of the cythchrome P450 CTP720B family in Stika spruce (Picea sitchensis)2009

    • Author(s)
      Hamberger, B., Ohnishi, T., Hamberger, B., Bohlman, J.
    • Organizer
      9th International Meeting : Biosynthesis and Function of Isoprenoids in Plants, Microorganisms and Parasites
    • Place of Presentation
      東京大学弥生講堂
    • Related Report
      2009 Annual Research Report
  • [Presentation] 植物の防御機構におけるイリドイド配糖体糖加水分解酵素の探索2009

    • Author(s)
      大西利幸, 松野聡, 渡辺修治
    • Organizer
      第44回植物化学調節学会
    • Place of Presentation
      東北大学片平キャンパス
    • Related Report
      2009 Annual Research Report
  • [Presentation] 茶の香気前駆体である配糖体生合成に関与する糖転移酵素の精製2009

    • Author(s)
      大西利幸, 谷田彩, 渡辺修治
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Related Report
      2009 Annual Research Report
  • [Presentation] オリーブにおけるオレウロペイン内生量の部位別・時期別変化および傷害応答による変動2009

    • Author(s)
      松野聡, 渡辺修治, 大西利幸
    • Organizer
      日本農芸化学会2010年度大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.grl.shizuoka.ac.jp/~t-oonishi/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.gri.shizuoka.ac.jp/~t-oonishi/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.grl.shizuoka.ac.jp/~t-oonishi/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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