Study of a new transgenic technique in coenocytic green algae utilizing their protoplast regeneration ability
Project/Area Number |
21880029
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
General fisheries
|
Research Institution | Hiroshima University |
Principal Investigator |
HIRAYAMA Makoto Hiroshima University, 大学院・生物圏科学研究科, 助教 (40535465)
|
Project Period (FY) |
2009 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥2,626,000 (Direct Cost: ¥2,020,000、Indirect Cost: ¥606,000)
Fiscal Year 2010: ¥1,222,000 (Direct Cost: ¥940,000、Indirect Cost: ¥282,000)
Fiscal Year 2009: ¥1,404,000 (Direct Cost: ¥1,080,000、Indirect Cost: ¥324,000)
|
Keywords | 遺伝子改変技術 / 海藻 / 細胞再構築現象 / ハネモ / 遺伝子改変 |
Research Abstract |
Reporter mRNA encoding Renilla luciferase was introduced into the sub-protoplasts, which were regenerated by squeezing out from the coenocytic green alga Bryopsis plumosa, using conventional transgenic methods with polyethyleneglycol and cationic lipid or a new method of which the reporter mRNA was added into the squeezing medium. The luciferase activities were detected in those sub-protoplasts introduced by the new method as well as by the conventional methods. These results indicated that a new easy transgenic technique in coenocytic green algae could be established.
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Report
(3 results)
Research Products
(1 results)