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Inhibitory Effects of Epigallocatechin-3 Gallate, a catechin in Green Tea, on Tumor-Associated Endothelial Cells

Research Project

Project/Area Number 21890006
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionHokkaido University

Principal Investigator

OHGA Noritaka  Hokkaido University, 大学院・歯学研究科, 特任助教 (40548202)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2010: ¥975,000 (Direct Cost: ¥750,000、Indirect Cost: ¥225,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
Keywords緑茶カテキン / 腫瘍血管新生 / 血管内皮前駆細胞 / 血管新生阻害薬
Research Abstract

The epigallocatechin-3 gallate (EGCG) in green tea suppresses tumor growth, but it is not known whether it specifically inhibits tumor angiogenesis. We examined the anti-angiogenic effect of EGCG on tumor-associated endothelial cells (TECs), EPCs, and normal endothelial cells (NECs). TECs were isolated and cultured from human tumor xenografts in nude mice. EGCG suppressed the migration of TECs and EPCs. EGCG also inhibited the phosphorylation of Akt in EPCs. MMP-9 in the bone marrow is involved in mobilizing bone marrow-derived VEGFR-2 positive cells into peripheral circulation. Expression of MMP-9 mRNA was suppressed in bone marrow stromal cells by EGCG. In vivo model, EGCG reduced melanoma growth. Our study showed that EGCG has anti-angiogenic effects. EGCG is a promising angiogenesis inhibitor for cancer therapy.

Report

(2 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • Research Products

    (30 results)

All 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (7 results) Remarks (2 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 2 results)

  • [Journal Article] Cyclooxygenase-2 inhibition causes antiangiogenic effects on tumor endothelial and vascular progenitor cells2011

    • Author(s)
      Muraki C., Ohga N., Hida Y., Nishihara H., Kato Y., Tsuchiya K., Matsuda K., Totsuka Y., Shindoh M., Hida K.
    • Journal Title

      Int J Cancer (in press)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] HuR keeps an angiogenic switch on by stabilizing mRNA of VEGF and COX-2 in tumor endothelium2011

    • Author(s)
      Kurosu T., Ohga N., Hida Y., Maishi N., Akiyama K., Kakuguchi W., Kuroshima T., Kondo M., Akino T., Totsuka Y., Shindoh M., Higashino F., Hida K.
    • Journal Title

      Br J Cancer 104(5)

      Pages: 819-829

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] HuR keeps an angiogenic switch on by stabilizing mRNA of VEGF and COX-2 in tumor endothelium2011

    • Author(s)
      Kurosu T.
    • Journal Title

      Br J Cancer

      Volume: 104 Pages: 819-829

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cyclooxygenase-2 inhibition causes antiangiogenic effects on tumor endothelial and vascular progenitor cells2011

    • Author(s)
      Muraki C.
    • Journal Title

      Int J Cancer

      Volume: (印刷中,印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crosstalk between blood vessels and tumor microenvironment2010

    • Author(s)
      Hida K., Ohga N., Kurosu T., Totsuka Y., Shindoh M.
    • Journal Title

      Oral Sci Int 7(1)

      Pages: 1-10

    • NAID

      10027107711

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and Culture of Microvascular Endothelial Cells from Murine Inguinal and Epididymal Adipose Tissue2010

    • Author(s)
      Kajimoto K., Hossen MN., Hida K., Ohga N., Akita H., Hyodo M., Hida Y., Harashima H.
    • Journal Title

      J Immunol Methods 357(1-2)

      Pages: 43-50

    • NAID

      120002248665

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolated tumor endothelial cells maintain specific character during long-term culture2010

    • Author(s)
      Matsuda K., Ohga N., Hida Y., Muraki C., Kurosu T., Tsuchiya K., Akino T., Shih SC, Totsuka Y., Klagsbrun M., Shindoh M., Hida K.
    • Journal Title

      Biochem Biophys Res Commun 394

      Pages: 947-954

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] BMP-9 induces proliferation of multiple types of endothelial cells in vitro and in vivo2010

    • Author(s)
      Suzuki Y., Ohga N., Morishita Y., Hida K., Miyazono K., Watabe T.
    • Journal Title

      J Cell Sci 123(10)

      Pages: 1684-1692

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Adrenomedullin antagonist suppresses tumor formation in renal cell carcinoma through inhibitory effects on tumor endothelial cells and endothelial progenitor mobilization2010

    • Author(s)
      Tsuchiya K., Hida K., Hida Y., Muraki C., Ohga N., Kondo T., Miseki T., Nakagawa K, Shindoh M., Harabayashi T., Shinohara N,, Nonomura K., Kobayashi M.
    • Journal Title

      Int J Oncol 36(6)

      Pages: 1379-1386

    • NAID

      120003370695

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolated tumor endothelial cells maintain specific character during long-term culture.2010

    • Author(s)
      Matsuda K.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 394 Pages: 947-954

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Adrenomedullin antagonist suppresses tumor formation in renal cellcarcinoma through inhibitory effects on tumor endothelial cells andendothelial progenitor mobilization2010

    • Author(s)
      Tsuchiya K.
    • Journal Title

      Int J Oncol

      Volume: 36 Pages: 1379-1386

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] BMP-9 induces proliferation of multiple types of endothelial cells in vitro and in vivo2010

    • Author(s)
      Suzuki Y.
    • Journal Title

      J Cell Sci

      Volume: 123 Pages: 1684-1692

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and Culture of Microvascular Endothelial Cells from Murine Inguinal and Epididymal Adipose Tissue2010

    • Author(s)
      Kajimoto K.
    • Journal Title

      J Immunol Methods

      Volume: 357 Pages: 43-50

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design of a dual-ligand system using a specific ligand and cell penetrating peptide, resulting in a synergistic effect on selectivity and cellular uptake2010

    • Author(s)
      Takara K.
    • Journal Title

      Int J Pharm

      Volume: 396 Pages: 143-148

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of Antiangiogenic Therapy in Head and Neck Cancer-Heterogeneity of Tumor Endothelium.2009

    • Author(s)
      Hida K., Ohga N., Hida Y., Shindoh M.
    • Journal Title

      Jpn Dent Sci Review 46

      Pages: 26-32

    • NAID

      10027210486

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Inhibitory Effects of Epigallocatechin-3 Gallate, a Polyphenol in Green Tea, on Tumor-Associated Endothelial Cells and Endothelial Progenitor Cells.2009

    • Author(s)
      Ohga N., Hida K., Hida Y., Muraki C., Tsuchiya K., Matsuda K., Ohiro Y., Totsuka Y., Shindoh M.
    • Journal Title

      Cancer Sci 100(10)

      Pages: 1963-1970

    • NAID

      10027196113

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] 緑茶カテキンEGCGは腫瘍血管内皮細胞ならびに血管内皮前駆細胞に対する抑制効果を有する2010

    • Author(s)
      大賀則孝
    • Organizer
      第64回日本口腔科学会学術集会
    • Place of Presentation
      札幌プリンスホテル(札幌)
    • Year and Date
      2010-06-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Comparative characterization of tumor endothelial cells isolated from highly and low metastatic tumors2010

    • Author(s)
      大賀則孝
    • Organizer
      第18回日本血管生物医学会学術集会
    • Place of Presentation
      大阪、梅田スカイビル
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comparative characterization of tumor endothelial cells isolated from highly and Iow metastatic tumors2010

    • Author(s)
      大賀則孝
    • Organizer
      第69回日本癌学会学術総会
    • Place of Presentation
      大阪、大阪国際会議場
    • Related Report
      2010 Annual Research Report
  • [Presentation] 緑茶カテキンEGCGは腫瘍血管内皮細胞ならびに血管内皮前駆細胞に対する抑制効果を有する2010

    • Author(s)
      大賀則孝
    • Organizer
      第64回日本口腔科学会学術集会
    • Place of Presentation
      札幌、札幌プリンスホテル
    • Related Report
      2010 Annual Research Report
  • [Presentation] 緑茶ポリフェノールは腫瘍血管内皮細胞と血管内皮前駆細胞に対する抑制抗効果を有する2009

    • Author(s)
      大賀則孝
    • Organizer
      第54回日本口腔外科学会総会・学術大会
    • Place of Presentation
      札幌コンベンションセンター(札幌)
    • Year and Date
      2009-10-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 緑茶ポリフェノールEGCGは腫瘍血管内皮に対する制御効果を有する2009

    • Author(s)
      大賀則孝
    • Organizer
      第17回日本血管生物医学会
    • Place of Presentation
      東京大学安田講堂(東京)
    • Year and Date
      2009-10-09
    • Related Report
      2010 Final Research Report
  • [Presentation] 緑茶ポリフェノールEGCGは腫瘍血管内皮に対する制御効果を有する2009

    • Author(s)
      大賀則孝
    • Organizer
      第68回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜(横浜)
    • Year and Date
      2009-10-02
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.den.hokudai.ac.jp/vascular-biology.com/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.vascular-biology.com/

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 腫瘍血管新生阻害剤2010

    • Inventor(s)
      樋田京子、樋田泰浩、大賀則孝
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-062825
    • Filing Date
      2010-03-10
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 腫瘍血管新生阻害剤2010

    • Inventor(s)
      樋田京子、樋田泰浩、大賀則孝
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-062831
    • Filing Date
      2010-03-10
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 腫瘍血管新生阻害剤2010

    • Inventor(s)
      大賀則孝
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-254205
    • Filing Date
      2010-11-12
    • Related Report
      2010 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 腫瘍血管新生阻害剤2010

    • Inventor(s)
      樋田京子
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-062825
    • Filing Date
      2010-03-18
    • Related Report
      2010 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 腫瘍血管新生阻害剤2010

    • Inventor(s)
      樋田京子
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-062831
    • Filing Date
      2010-03-18
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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