|Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000)
Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
The purpose of our study is to clarify roles of Notch signal of NK cell in graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation. Functionally mature NK cells were induced from human cord-blood CD34-positive cells by stimulation with Notch ligand. We established allogeneic stem cell transplantation GVHD model. Diminishment of GVHD was observed when Notch-induced NK cells were transfused into the recipient. It is revealed that NK cells of patients after hematopoietic stem-cell transplantation expressed Notch1 and intestinal epithelial cells, which are targets of GVHD, expressed Notch ligand, Jagged1. This result suggests the relation between Notch signal of NK cell and GVHD.