Evaluation of molecular mechanisms of tumor-specific protoporphyr in accumulation induced by 5-aminolevulinic acid
| Project/Area Number |
21890084
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| Research Category |
Grant-in-Aid for Research Activity start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Biological pharmacy
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| Research Institution | Kanazawa University |
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Principal Investigator |
NAKANISHI Takeo Kanazawa University, 薬学系, 准教授 (30541742)
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| Co-Investigator(Renkei-kenkyūsha) |
TAMAI Ikumi 金沢大学, 薬学系, 教授 (20155237)
SHIRASAKA Yoshiyuki 金沢大学, 薬学系, 助教 (60453833)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
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| Keywords | トランスポーター / アミノレブリン酸 / プロトポルフィリン / 化学療法 / フェロキラターゼ / 光力学療法 / 有機アニオン / プロトポルフォリン / 薬物排泄 / 光力学診断 / 蛍光診断 |
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| Research Abstract |
5-Aminolevulinic acid is one of the most potent photodynamic therapeutic agents, because it induces tumor cell-specific intracellular accumulation of a photosensitizer, protoporphyrin IX (PPIX). However, molecular mechanisms of such 5-ALA-induced PPIX accumulation remain unclear. In the current study, in order to establish a basis to predict efficacy of photodynamic therapy to treat cancer, molecular mechanisms of intracellular accumulation of PPIX induced by 5-ALA were studied in various human cancer cell lines exposed to 5-ALA. Results suggested that PPIX accumulation is likely determined by PPIX biosynthesis, ferrochelatase (FECH) activity and PPIX efflux.
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Report
(3 results)
Research Products
(2 results)
