functional analysis of GslA that newly identified of P.gingivalis

Research Project

Project/Area Number	21890195
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Periodontal dentistry
Research Institution	Nagasaki University
Principal Investigator	HARUYAMA Koki Nagasaki University, 大学病院, 医員 (40549466)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000) Fiscal Year 2010: ¥1,235,000 (Direct Cost: ¥950,000、Indirect Cost: ¥285,000) Fiscal Year 2009: ¥1,365,000 (Direct Cost: ¥1,050,000、Indirect Cost: ¥315,000)
Keywords	P. gingivalis / gingipain / 自然免疫 / P.gingivalis / 刺激伝達経路 / GslA
Research Abstract	We previously purified and identified the protein from cell surface component on P. gingivalis, a major periodontal pathogen. This protein was able to induce Toll-like receptor 2 (TLR2)- and TLR4-independent signaling in CHO cells and that this component can be degraded by gingipains. Therefore, we named the protein as gingipain-sensitive ligand A (GslA). P. gingivalis gingipain-null mutant was partially inhibited by antiserum against a recombinant protein expressed from the first one third of gslA. Furthermore, the gslA gene was present in four of seven P. gingivalis strains tested.