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Abnormal lipid metabolism and disease caused by deficiency of atypical secretory phospholipase A2 (sPLA2-III)

Research Project

Project/Area Number 21890310
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionTokyo Metropolitan Organization for Medical Research

Principal Investigator

SATO Hiroyasu  Tokyo Metropolitan Organization for Medical Research, 東京都臨床医学総合研究所, 研究員 (50546629)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,652,000 (Direct Cost: ¥2,040,000、Indirect Cost: ¥612,000)
Fiscal Year 2010: ¥1,261,000 (Direct Cost: ¥970,000、Indirect Cost: ¥291,000)
Fiscal Year 2009: ¥1,391,000 (Direct Cost: ¥1,070,000、Indirect Cost: ¥321,000)
Keywords脂質 / ホスホリパーゼA2 / 生殖 / メタボリックシンドローム / メタボリックシンドロ
Research Abstract

We have shown that group III phospholipase A2 (sPLA2-III), a member of the secreted phospholipase A2 (sPLA2) family, is expressed in the mouse proximal epididymal epithelium and that targeted disruption of the gene encoding this protein (Pla2g3) leads to defects in sperm maturation and fertility. Our results reveal a role for the atypical sPLA2 family member sPLA2-III in epididymal lipid homeostasis and indicate that its perturbation may lead to sperm dysfunction. Additionally, sPLA2-III was expressed constitutively in preadipocytic cells in the stromal vascular fraction of white adipose tissue. High-fat diet induced obesity, steatohepatitis, hyperlipidemia, hyperglycemia and insulin resistance were ameliorated in Pla2g3-/- mice. Adipose or hepatic expression of lipogenic, adipogenic and proinflammatory genes and adipose level of lysophosphatidylcholine were significantly reduced in Pla2g3-/- mice compared with wild-type mice.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (35 results)

All 2011 2010 2009 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (23 results) Remarks (3 results)

  • [Journal Article] Hair follicular expression and function of group X secreted phospholipase A_2 in mouse skin.2011

    • Author(s)
      Yamamoto K, Taketomi T, Isogai Y, Miki Y, Sato H, Masuda S, Nishito Y, Morioka K, Ishimoto Y, Suzuki N, Yokoya Y, Hanasaki K, Ishikawa Y, Ishii T, Kobayashi T, Fukami K, Ikeda K, Nakanishi H, Taguchi R, Murakami M
    • Journal Title

      J Biol Chem. 286

      Pages: 11616-11631

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physiological roles of group X secreted phospholipase A_2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function.2011

    • Author(s)
      Sato H, Isogai Y, Masuda S, Taketomi Y, Miki Y, Kamei D, Hara S, Kobayashi T, Ishikawa Y, Ishii T, Ikeda K, Taguchi R, Ishimoto Y, Suzuki N, Yokota Y, Hanasaki K, Suzuki-Yamamoto T, Yamamoto K, Murakami M
    • Journal Title

      J Biol Chem. 286

      Pages: 11632-11648

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recent progress in phospholipase A_2 research : from cells to animals to humans.2011

    • Author(s)
      Murakami M, Taketomi Y, Miki Y, Sato H, Hirabayashi T, Yamamoto K
    • Journal Title

      Prog Lipid Res. 50

      Pages: 152-192

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Physiological roles of group X secreted phospholipase A_2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function2011

    • Author(s)
      Sato, et al
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 286 Pages: 11632-11648

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Group III secreted phospholipase A_2 regulates epididymal sperm maturation and fertility in mice.2010

    • Author(s)
      Sato H, Taketomi T, Isogai Y, Miki Y, Yamamoto K, Masuda S, Hosono T, Arata S, Ishikawa Y, Ishii T, Kobayashi T, Nakanishi H, Ikeda K, Taguchi R, Hara S, Kudo I, Murakami M
    • Journal Title

      J Clin Invest. 120

      Pages: 1400-1414

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Group X phospholipase A_2 is released during sperm acrosome reaction and controls fertility outcome in mice.2010

    • Author(s)
      Escoffier J, Jemel I, Tanemoto A, Taketomi Y, Payre C, Coatrieux C, Sato H, Yamamoto K, Masuda S, Pernet-Gallay K, Pierre V, Hara S, Murakami M, De Waard M, Lambeau G, Arnoult C
    • Journal Title

      J Clin Invest. 120

      Pages: 1415-1428

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Group III secreted phospholipase A2 regulates epididymal sperm maturation and fertility in mice2010

    • Author(s)
      Hiroyasu Sato
    • Journal Title

      The Journal of clinical investigation (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Group III secreted phospholipase A_2 transgenic mice spontaneously develop inflammation.2009

    • Author(s)
      Sato H, Taketomi Y, Isogai Y, Masuda S, Kobayashi T, Yamamoto K, Murakami M
    • Journal Title

      Biochem J. 421

      Pages: 17-27

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Group III secreted phospholipase A2 transgenic mice spontaneously develop inflammation2009

    • Author(s)
      Hiroyasu Sato
    • Journal Title

      The Biochemical journal 421

      Pages: 17-27

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 「生命薬学における精子バイオロジーと脂質ネットワーク」ホスホリパーゼA_2による精子成熟と活性化の制御2011

    • Author(s)
      佐藤弘泰、村上誠
    • Organizer
      日本薬学会第131年会シンポジウム
    • Place of Presentation
      静岡市
    • Year and Date
      2011-03-29
    • Related Report
      2010 Final Research Report
  • [Presentation] 「生命薬学における精子バイオロジーと脂質ネットワーク」ホスホリパーゼA_2による精子成熟と活性化の制御2011

    • Author(s)
      佐藤弘泰
    • Organizer
      日本薬学会第131年会
    • Place of Presentation
      静岡(招待講演)
    • Year and Date
      2011-03-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] フタル酸モノエステル類のホスホリパーゼA_2活性阻害を介したマウス精子受精能の抑制2011

    • Author(s)
      原俊太郎、種本明生、加藤洋幸、佐藤弘泰、武富芳隆、村上誠
    • Organizer
      第81回日本衛生学会学術総会
    • Place of Presentation
      東京
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] Suppression of sperm fertility by monophthalate via inhibiting spermatozoal phospholipase A_2 activity.2011

    • Author(s)
      Hara S, Taketomi Y, Sato H, Murakami M
    • Organizer
      SOT Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] sPLA_2-V, an adipocyte-inducible metabolic sPLA_2, controls systemic lipid metabolism.2010

    • Author(s)
      佐藤弘泰、武富芳隆、山本圭、石川由紀雄、石井寿晴、原俊太郎、村上誠
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] sPLA_2-III, a mast cell-derived anaphylactic sPLA_2, regulates mast cell-dependent anaphylactic response.2010

    • Author(s)
      武富芳隆、佐藤弘泰、田中智之、中村正則、上野紀子、西藤泰昌、山本圭、杉本幸彦、原俊太郎、村上誠
    • Organizer
      BMB2010
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-10
    • Related Report
      2010 Final Research Report
  • [Presentation] sPLA_2-V, an adipocyte-inducible metabolic sPLA_2, controls systemic lipid metabolism.2010

    • Author(s)
      佐藤弘泰
    • Organizer
      Biochemistry and Molecular Biology BMB2010
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2の欠損はメタボリックシンドロームを改善する2010

    • Author(s)
      佐藤弘泰、磯貝有紀、武富芳隆、山本圭、石川由紀雄、石井壽晴、小林哲幸、中西広樹、池田和貴、田口良、原俊太郎、村上誠
    • Organizer
      第52回日本脂質生化学会
    • Place of Presentation
      群馬
    • Year and Date
      2010-06-15
    • Related Report
      2010 Final Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2の欠損はメタボリックシンドロームを改善する2010

    • Author(s)
      佐藤弘泰
    • Organizer
      第52回日本脂質生化学会
    • Place of Presentation
      群馬県伊香保
    • Year and Date
      2010-06-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] Group III secreted phospholipase A_2 regulates epididymal sperm maturation and fertility in mice2010

    • Author(s)
      Hiroyasu Sato
    • Organizer
      Keystone Symposia
    • Place of Presentation
      ウェスティン都ホテル京都
    • Year and Date
      2010-06-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] New insights into the functions of secreted phospholipase A_2s : sPLA_2 in reproduction.2010

    • Author(s)
      Sato H, Taketomi Y, Yamamoto K, Arnoult C, Lambeau G, Murakami M
    • Organizer
      7th GERLI Lipidmics Congress
    • Place of Presentation
      France
    • Related Report
      2010 Final Research Report
  • [Presentation] Group III secreted phospholipase A_2 regulates epididymal sperm maturation and fertility in mice.2010

    • Author(s)
      Sato H, Taketomi Y, Yamamoto K, Murakami M
    • Organizer
      Keystone Symposia.
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Recent insights into the multiple molecular and functional properties of group X sPLA_2.2010

    • Author(s)
      Lambeau G, Jemel J, Escoffier J, Ait-Oufella H, Hiromi Li H, Guillaume C, Gora S, Surrel F, Coatrieux C, Payre C, Taketomi Y, Sato H, Yamamoto K, Arnoult C, Mallat Z, Gelb M.H, Deregnaucourt C, Ninio E, Karabina S, Murakami M.
    • Organizer
      Keystone Symposia
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2 は精子成熟を制御する2009

    • Author(s)
      佐藤弘泰、武富芳隆、山本圭、磯貝有紀、三木寿美、小林哲幸、池田和貴、中西広樹、田口良、原俊太郎、村上誠
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
    • Related Report
      2010 Final Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2はマスト細胞の分化成熟を制御する2009

    • Author(s)
      武富芳隆、佐藤弘泰、田中智之、上野紀子、山本圭、原俊太郎、村上誠
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
    • Related Report
      2010 Final Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2は精子成熟を制御する2009

    • Author(s)
      佐藤弘泰
    • Organizer
      第82回生化学会大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2009-10-21
    • Related Report
      2009 Annual Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2は精子成熟を制御する2009

    • Author(s)
      佐藤弘泰、武富芳隆、磯貝有紀、三木寿美、山本圭、石川由紀雄、石井壽晴、小林哲幸、中西広樹、池田和貴、田口良、原俊太郎、村上誠
    • Organizer
      第29回日本脂質生化学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-07-31
    • Related Report
      2010 Final Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2はマスト細胞依存性の即時性アレルギーに関わる2009

    • Author(s)
      武富芳隆、佐藤弘泰、田中智之、上野紀子、山本圭、田口良、工藤一郎、原俊太郎、村上誠
    • Organizer
      第29回日本脂質生化学会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-07-31
    • Related Report
      2010 Final Research Report
  • [Presentation] III型分泌性ホスホリパーゼA_2は精子成熟を制御する2009

    • Author(s)
      佐藤弘泰
    • Organizer
      第51回 日本脂質生化学会
    • Place of Presentation
      名古屋 ウィルあいち
    • Year and Date
      2009-07-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Impaired sperm maturation and fertility in group III phospholipase A_2-defficient mice2009

    • Author(s)
      佐藤弘泰
    • Organizer
      4^<th> International Conference on Phosphplipase A_2 and Lipid Mediatoers
    • Place of Presentation
      東京 学術総合センター
    • Year and Date
      2009-05-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] Impaired sperm maturation and fertility in group III phospholipase A_2-deficient mice.2009

    • Author(s)
      Sato H, Taketomi Y, Isogai Y, Yamamoto K, Murakami M
    • Organizer
      The 4th International Conference on Phospholipase A_2s and Lipid Mediators
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Group III phospholipase A_2 in stromal fraction of adipose tissue controls systemic lipid metabolism.2009

    • Author(s)
      Isogai Y, Sato H, Taketomi Y, Yamamoto K, Kobayashi T, Murakami M
    • Organizer
      The 4th International Conference on Phospholipase A_2s and Lipid Mediators
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] Group III secreted phospholipase A_2, an endogenous bee venom phospholipase A_2 homolog, is a crucial regulator of mast cell maturation and functions.2009

    • Author(s)
      Taketomi Y, Sato H, Ueno N, Yamamoto K, Murakami M
    • Organizer
      The 4th International Conference on Phospholipase A_2s and Lipid Mediators
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.igakuken.or.jp/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.rinshoken.or.jp/P/index_japanese.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.rinshoken.or.jp/P/index_japanese.html

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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