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Clarification of crosstalk between ABA signal transduction and optical response on anthocyanin and sugar accumulation in grape berries

Research Project

Project/Area Number 21H02182
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionChiba University

Principal Investigator

KONDO Satoru  千葉大学, 大学院園芸学研究院, 特任研究員 (70264918)

Co-Investigator(Kenkyū-buntansha) 池浦 博美  島根大学, 学術研究院農生命科学系, 准教授 (10440158)
齋藤 隆徳  千葉大学, 大学院園芸学研究院, 助教 (20753479)
森口 卓哉  静岡県立農林環境専門職大学, 生産環境経営学部, 教授 (80343945)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2023: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Keywords植物ホルモン / アブシシン酸 / 光受容体 / ABA代謝 / ABAシグナル伝達 / ブドウ / 糖濃度 / ABA / 糖転流 / 光応答 / アントシアニン / 糖 / NDGA / 青色光 / アントシアニン合成 / 糖代謝
Outline of Research at the Start

果実におけるABA代謝、ABAシグナル伝達、光波長・光応答のアントシアニン、糖および香気成分産生ならびに果実への光合成産物転流について、これらの相対的な関与深度および本質性の解明を行う。 環境からのストレスおよびABAに関連するDNAのメチル化の着色および糖合成に及ぼす機構を詳細に解明する。葉の内生ABA代謝およびABAシグナル伝達の影響を解明する。本研究では内生ABA代謝、ABAシグナル伝達および光応答のクロストークを解明し、各々が必要とされる時期に最大効果が発揮されるよう制御し、色・糖・香りを大きく向上させて市場価値を高め、社会的にインパクトを与えるブドウの生産技術を開発する。

Outline of Final Research Achievements

We investigated the effects of ABA metabolism, ABA signal transduction, and light wavelength on sugar synthesis in grapes. Endogenous ABA concentrations were highest in the red LED irradiation area, while anthocyanin and sugar concentrations were highest in the blue LED irradiation area.
When 13C stable isotope was incorporated into the leaves and the translocation of 13C to the cluster was measured, there was a significant increase in the blue irradiated plots compared to the untreated plots. Blue LED irradiation also stimulated the SNRK2.6, ABF2, and PP2C9 genes involved in ABA signal transduction, as well as the photoreceptor phototropin and cryptochrome gene expression. These results suggest that blue light may stimulate ABA signals through photoreceptors.

Academic Significance and Societal Importance of the Research Achievements

ブドウなど果実の発育にとって光は必須要素であるが、波長が果実の糖合成に及ぼす影響については必ずしも明らかでなかった。本研究ではブドウの成熟に関連する植物ホルモンであるアブシシン酸と光受容体の関係を検討し、これらが葉から果房への糖転流に関連することを示唆した。
ブドウ果実の品質向上のために補光処理が行われているが、青色光照射が有効であり、これは青色光が光受容体であるファトトロピンおよびクリプトクローム発現を刺激し、その結果アブシシン酸シグナル伝達遺伝子発現が刺激され、糖転流に関するスクローストランスポーターなど遺伝子発現が刺激されたことが示唆され、糖増加の機構の一端が解明されたと考えられる。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • Research Products

    (7 results)

All 2024 2023 2022

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (3 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] Effect of varying concentrations of melatonin on anthocyanin and sugar metabolism in grapes (Vitis labruscana L.)2023

    • Author(s)
      Wang, S. , Song, J. , Wang, L. , ... Srilaong, V. , Kondo, S.
    • Journal Title

      Agriculture and Natural Resources,

      Volume: 57 Issue: 1 Pages: 107-116

    • DOI

      10.34044/j.anres.2023.57.1.11

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retardation of ABA 8'-hydroxylase affects endogenous ABA, sugar metabolism, and aroma volatiles in grape berries2023

    • Author(s)
      Thunyamada, S. , Ohkawa, K. , Ohara, H. , ... Todoroki, Y. , Kondo, S.
    • Journal Title

      European Journal of Horticultural Science,

      Volume: 88 Issue: 3 Pages: 1-12

    • DOI

      10.17660/ejhs.2023/019

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABA signaling and metabolism on anthocyanin and sugar syntheses in grapes2022

    • Author(s)
      Kondo, S. , Hoshi, C. , Tomiyama, H. , ... Ohara, H. , Iwasaki, N.
    • Journal Title

      Acta Horticulturae

      Volume: 1344 Issue: 1344 Pages: 45-53

    • DOI

      10.17660/actahortic.2022.1344.8

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Retardation of Endogenous ABA Synthesis by NDGA in Leaves Affects Anthocyanin, Sugar, and Aroma Volatile Concentrations in ‘Kyoho’ Grape Berries2022

    • Author(s)
      Saito Takanori、Tomiyama Hiroyuki、Ishioka Miho、Hashimoto Natsuki、Thunyamada Sorawee、Ohkawa Katsuya、Ohara Hitoshi、Ikeura Hiromi、Kondo Satoru
    • Journal Title

      The Horticulture Journal

      Volume: 91 Issue: 2 Pages: 186-194

    • DOI

      10.2503/hortj.UTD-338

    • NAID

      130008145466

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Presentation] Blue-Light Irradiation Stimulates Abscisic Acid Signaling and Sugar Translocation in Vitis labluscana grapevines2024

    • Author(s)
      Satoru Kondo
    • Organizer
      ISHS
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of Blue LED irradiation on sugar concentration2023

    • Author(s)
      Satoru Kondo
    • Organizer
      ISHS
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ABA signaling and metabolism on anthocyanin and sugar syntheses in grapes2022

    • Author(s)
      Satoru Kondo
    • Organizer
      ISHS
    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Int'l Joint Research

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Published: 2021-04-28   Modified: 2025-01-30  

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