• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of a new assessment method using environmental DNA to elucidate the impact of global warming countermeasures on lake ecosystems

Research Project

Project/Area Number 21K12273
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 63040:Environmental impact assessment-related
Research InstitutionMatsuyama University

Principal Investigator

Tsugeki Narumi  松山大学, 法学部, 教授 (20423618)

Co-Investigator(Kenkyū-buntansha) 鏡味 麻衣子  横浜国立大学, 大学院環境情報研究院, 教授 (20449250)
土居 秀幸  京都大学, 情報学研究科, 教授 (80608505)
本庄 三恵  京都大学, 生態学研究センター, 准教授 (30450208)
加 三千宣  愛媛大学, 沿岸環境科学研究センター, 准教授 (70448380)
Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords堆積物DNA / 動物プランクトン / 琵琶湖 / 過去100年 / 遺伝的多様性 / 外来種 / 植物プランクトン / 古陸水学 / カイアシ類 / 温暖化 / 水位操作 / 近過去 / 環境DNA / 湖沼 / 寄生者 / 環境変化 / 微生物
Outline of Research at the Start

温暖化の進行で懸念される異常豪雨の頻発化に備え施行された水位操作は湖沼生態系にどのような影響を与えているだろうか。本研究は環境DNAを用いてこれまで復元ができなかった微生物相の変動を再現する手法の高精度化を推進する。そして魚類資源の餌である動物・植物プランクトンとこれらプランクトン動態に影響を及ぼす寄生者(ツボカビ・ウイルス)の過去100年にわたる微生物相の変動を再現することで、温暖化や異常気象に備えた水位調節の対策が琵琶湖生態系に及ぼした波及効果を解明することを目的とする。

Outline of Final Research Achievements

We applied environmental DNA (eDNA) techniques to sediment samples in Lake Biwa to reconstruct the historical dynamics of copepods (Eodiaptomus japonicus) and Daphnia (Daphnia galeata, D. pulicaria), which are important zooplankton as key food resources for fish but their long-term dynamics over several decades remains largely limited due to the lack of long-term monitoring data. Copepod eDNA in the sediments (sedDNA) was continuously detected over the past 100 years, with concentrations varying over time. The sedDNA concentration correlated significantly with in situ production, biomass but not with resting eggs or specific growth rate. While Daphnia sedDNA concentrations correlated significantly with their resting egg production rather than the biomass. These results provide evidence that zooplankton sedDNA can be an effective tool for tracking past population dynamics while biological information for the reconstructed sedDNA concentrations differs among species.

Academic Significance and Societal Importance of the Research Achievements

堆積試料に残る環境DNA(堆積物DNA)の活用は、これまで得られなかった過去の生物相を含めた生態系変化の実態解明につながるもので、過去から現在を俯瞰する新たなモニタリング手法としての展開が期待される。本課題は、従来の古生物学的手法では過去情報が得られなかった魚類の餌資源として重要な動物プランクトンのカイアシ類について、堆積物DNAを活用することで現存量を捉えるツールとしての有効性を示し、本分野の進展に大きく貢献した。またミジンコ類に関しては、休眠卵量の変遷を迅速かつ簡便に捉えられることを示し、堆積物DNAによる動物プランクトンの新たなモニタリング手法の実現可能性を示すことができた。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (20 results)

All 2024 2023 2022 2021 Other

All Int'l Joint Research (3 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 4 results) Presentation (12 results) (of which Int'l Joint Research: 4 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] Umea University(スウェーデン)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Umea University(スウェーデン)

    • Related Report
      2022 Research-status Report
  • [Int'l Joint Research] SLU(スウェーデン)

    • Related Report
      2021 Research-status Report
  • [Journal Article] Establishment success of alien Daphnia in the ancient Lake Biwa: Insights from sedimentary archives2024

    • Author(s)
      Narumi Tsugeki, Isana Hashimoto, Kai Nakane, Mie N. Honjo, Kimiko Uchii.
    • Journal Title

      Hydrobiologia

      Volume: - Issue: 15 Pages: 3591-3602

    • DOI

      10.1007/s10750-024-05519-0

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sedimentary DNA can reveal the past population dynamics of a pelagic copepod2023

    • Author(s)
      Kai Nakane, Xin Liu, Hideyuki Doi, Gael Dur, Michinobu Kuwae, Syuhei Ban, Narumi Tsugeki
    • Journal Title

      Freshwater Biology

      Volume: - Issue: 11 Pages: 1917-1928

    • DOI

      10.1111/fwb.14096

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Investigating the effects of anthropogenic stressors on lake biota using sedimentary DNA2023

    • Author(s)
      Barouillet Cecilia、Monchamp Marie‐Eve、Bertilsson Stefan、Brasell Katie、Domaizon Isabelle、Epp Laura S.、Ibrahim Anan、Mejbel Hebah、Nwosu Ebuka Canisius、Pearman John K.、Picard Mailys、Thomson‐Laing Georgia、Tsugeki Narumi、Von Eggers Jordan、Gregory‐Eaves Irene、Pick Frances、Wood Susanna A.、Capo Eric
    • Journal Title

      Freshwater Biology

      Volume: - Issue: 11 Pages: 1799-1817

    • DOI

      10.1111/fwb.14027

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Reconstruction of 100-year dynamics in Daphnia spawning activity revealed by sedimentary DNA.2022

    • Author(s)
      Narumi Tsugeki, Kai Nakane, Hideyuki Doi, Natsuki Ochi and Michinobu Kuwae
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 1741-1741

    • DOI

      10.1038/s41598-021-03899-0

    • Related Report
      2021 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Sedimentary DNA can reveal past pelagic copepod population dynamics2023

    • Author(s)
      Narumi Tsugeki, Kai Nakane, Xin Liu, Hideyuki Doi, Gael Dur, Michinobu Kuwae, Syuhei Ban
    • Organizer
      XXI INQUA Congress
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Zooplankton sedimentary DNA as an effective tool for tracking past population dynamics2023

    • Author(s)
      Narumi Tsugeki, Kai Nakane, Hideyuki Doi, Kazuaki Tadokoro, Michinobu Kuwae
    • Organizer
      The eDNA Society International Meeting
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 琵琶湖から広がる泳ぐカビの世界:ツボカビの謎に迫る2023

    • Author(s)
      鏡味麻衣子
    • Organizer
      第22回生態学琵琶湖賞授賞式・受賞記念公演
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Role of saprotrophic chytrids on two large desmids (Staurastrum and Micrasterias) in Lake Biwa2023

    • Author(s)
      Duangmany Phongsa, Tatsuro Hashizume, Kensuke Seto, Ban Syuhei, Kagami Maiko
    • Organizer
      日本陸水学会第87回
    • Related Report
      2023 Annual Research Report
  • [Presentation] 泳ぐカビの世界:ツボカビの謎に迫る2023

    • Author(s)
      鏡味麻衣子
    • Organizer
      明治大学科学技術研究所 公開講演会「パラサイト;生態系の舞台裏」
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] 堆積物DNAに基づくカイアシ類の長期変動に関する研究2022

    • Author(s)
      中根 快, リュウ キン, 土居 秀幸, 伴 修平, デュア・ガエル, 加 三千宣, 槻木 玲美
    • Organizer
      日本陸水学会 第86回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 堆積物記録から復元された琵琶湖の動・植物プランクトン動態 :水位管理・気象と栄養塩変化の影響2022

    • Author(s)
      槻木 玲美, Eric Capo, 越智 梨月, 鏡味 麻衣子, 土居 秀幸, 早川 和秀, 加 三千宣
    • Organizer
      日本陸水学会 第86回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 堆積物環境DNAによる琵琶湖魚類の過去復元2022

    • Author(s)
      坂田雅之, 槻木 玲美, 加 三千宣, 土居 秀幸, 源 利文
    • Organizer
      日本陸水学会 第86回大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Environmental DNA in lake sediment provides fish information on reconstructing past fauna in lake ecosystems.2022

    • Author(s)
      Sakata M. K., Tsugeki N., Kuwae M., Doi H., Minamoto T.
    • Organizer
      British Ecological Sciety Annual Meeting
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] 堆積物DNAにより復元されたミジンコの過去100年にわたる産卵量の変動2022

    • Author(s)
      槻木玲美, 中根 快, 土居 秀幸, 加 三千宣
    • Organizer
      日本生態学会第69回大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Environmental-driven changes in the microbial eukaryotic community of Lake Biwa over the last 120 years2022

    • Author(s)
      Eric Capo, Stefan Bertilsson, Kai Nakane, Maiko Kagami, Michinobu Kuwae, Narumi Tsugeki
    • Organizer
      Joint Aquatic Science Meeting
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] 堆積物DNAにより復元された動物プランクトンの過去100年にわたる産卵量の変動2021

    • Author(s)
      中根快, 土居秀幸, 越智梨月, 加三千宣, 槻木玲美
    • Organizer
      環境DNA学会 第4回
    • Related Report
      2021 Research-status Report
  • [Remarks] Eukaryotic community of Lake Biwa over 120 years

    • URL

      https://www.researchgate.net/publication/360484687_Environmental-driven_changes_in_the_microbial_eukaryotic_community_of_Lake_Biwa_over_the_last_120_years

    • Related Report
      2022 Research-status Report

URL: 

Published: 2021-04-28   Modified: 2025-01-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi