Preparation and characterization of carbon nanomaterials prepared by thermal treatment of biomass DNA
| Project/Area Number |
21K12306
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 64030:Environmental materials and recycle technology-related
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| Research Institution | Nagoya University |
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Principal Investigator |
ジンチェンコ アナトーリ 名古屋大学, 環境学研究科, 准教授 (00432352)
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| Project Period (FY) |
2021-04-01 – 2024-03-31
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| Project Status |
Completed (Fiscal Year 2023)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2023: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | DNA / biomass / carbon nanomaterials / fluorescent nanomaterial / biodots / pyrolysis / microwave processing / metal ion sensing / mercury ion sensing / ナノ材料 / 水熱合成 / マイクロ波処理 / 熱分解 |
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| Outline of Research at the Start |
現在、糖由来のバイオマスがナノ材料の作製のため幅広く利用される一方、自然界に豊富に存在する核酸（DNA）からの材料作製技術がほとんど無い。本研究では、魚の白子から抽出させるDNAなどを用いて、DNAの水熱処理、マイクロ波処理および加熱分解処理によりカーボンナノ材料の作製技術を開発する。得られたナノ材料の特性評価を行い、吸着分離、センシング、バイオイメージングなどの分野において応用性を検討する。
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| Outline of Annual Research Achievements |
The key objective of the project was to make clear the possibility of utilizing waste-derived DNA for the preparation of functional materials using the thermal processing method. The HT synthesis of DNA biodots using a conventional HT reactor and a microwave reactor under the same conditions was compared and it was shown that biodots prepared using the microwave reactor had 1.5-2-fold higher fluorescence.
The process of nucleic acids thermal decomposition by hydrothermal and pyrolytic methods was systematically investigated. The synthetic methods for preparation of fluorescent nanoparticles (biodots) from nucleic acids, DNA and RNA were elaborated and the possibility to control the fluorescent intensity and fluorescence color of product was demonstrated. The mechanism of nucleic acid biodots formation was shown to include degradation of ribose sugar, depurination, depyrimidation and consequent fusion of heteroaromatic nucleobases. The structure of biodots was clarified.
The application of nucleic acids for analytical detection and Hg2+, Ag+, and Cu2+ ions at ppm concentrations as well as bioimaging was demonstrated. In contrast, the application of DNA thermal decomposition products such as biochars for adsorption and catalytic applications was shown to be limited.
The conducted research provided important knowledge on the scope and limitations of DNA biomass-derived products prepared by thermal processing and represents a valuable technology in addition to the existing sustainable biomass processing methods.
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Report
(3 results)
Research Products
(11 results)
