A novel design methodology for the construction of functionalized human cell monolayer on an egg

Research Project

Project/Area Number	21K12667
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 90110:Biomedical engineering-related
Research Institution	Kyushu University
Principal Investigator	黄文敬九州大学, 工学研究院, 特任助教 (00633413)
Co-Investigator(Kenkyū-buntansha)	張宏東海大学, 工学部, 特定研究員 (80774257)
Project Period (FY)	2021-04-01 – 2024-03-31
Project Status	Granted (Fiscal Year 2021)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	Chorioallantoic membrane / Epithelial cell removal / Human organs on an egg / Human cell - chick heart
Outline of Research at the Start	The research will locally remove the monolayer of chick epithelial cells on the surface of the chorioallantoic membrane of an egg. We will culture a monolayer of human epithelial/endothelial cells on the egg. The barrier functions of the human cells on the chick embryo membrane will be clarified.