Understanding the effects of substrate stiffness on rejuvenation of aging MSCs via single cell analysis.

Research Project

Project/Area Number	21K12686
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 90120:Biomaterials-related
Research Institution	Kyushu University
Principal Investigator	久保木タッサニーヤー九州大学, 先導物質化学研究所, 助教 (20526834)
Project Period (FY)	2021-04-01 – 2024-03-31
Project Status	Granted (Fiscal Year 2021)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2023: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2022: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2021: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	MSC aging / mechano-regulation / substrate stiffness / redox balance / single cell analysis
Outline of Research at the Start	The key question for this proposal is how the substrate stiffness could maintain the MSC quality and delay the aging. The central hypothesis is that the compliance substrates could reduce CSKs tension, increase their dynamics and the activate the antioxidant system to maintain the redox homeostasis. Increasing of aging population and aged-related diseases increase the health problems. Identification of key targets for preventing or ameliorating senescence is essential for precisely engineered mechanical substrate to slow or reverse the age-related reduction of MSC properties.