Exploring the functional role of Hijiki in obesity-associated to sphingolipid metabolism

Research Project

Project/Area Number	21K14812
Research Category	Grant-in-Aid for Early-Career Scientists
Allocation Type	Multi-year Fund
Review Section	Basic Section 38050:Food sciences-related
Research Institution	Hokkaido University
Principal Investigator	BommeGowda SiddabasaveGowda 北海道大学, 保健科学研究院, 講師 (50800504)
Project Period (FY)	2021-04-01 – 2025-03-31
Project Status	Granted (Fiscal Year 2021)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2024: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2023: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2022: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	Hijiki / Obesity / Sphingolipid metabolism
Outline of Research at the Start	(1) Isolate and characterize the chemical constituent of Hijiki responsible for SMS inhibition. (2) Determination of its IC50 and mode of action on the lipid droplet formation by cell-based assay (3) Revealing the in vitro effects by analysis of altered cellular lipid biomarkers by LC-MS (4) Elucidate the in vivo effects by oral administration of the active constituent of Hijiki using High-fat diet mouse model.