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Investigation of translational factor pool formed around the ribosomal P-stalk

Research Project

Project/Area Number 21K15015
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 43010:Molecular biology-related
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Imai Hirotatsu  琉球大学, 医学(系)研究科(研究院), 助教 (00866668)

Project Period (FY) 2021-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2023: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2022: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2021: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords翻訳 / リボソーム / RNA / リボソームストーク / 近位依存ビオチン標識法
Outline of Research at the Start

リボソームは「ストーク」と呼ばれる柔軟な機能ドメインを用いて、多種多様な翻訳因子と一過的な相互作用を繰り返しながら正確にタンパク質を合成する。本研究では、リボソームのストーク周囲に形成される翻訳因子プールの細胞内における構成因子を網羅的に探索する。またストークと結合する翻訳因子とストークの結合動態を明らかにする。これにより、混雑した細胞内において効率よく翻訳が制御される分子基盤を、ストークが形成する「翻訳の反応場」の観点から明らかにする。

Outline of Final Research Achievements

In this project, we focused on the ribosomal P-stalk, which consists of ribosomal proteins P0, P1, and P2, that form translational GTPase factor pool for efficient translation. We tried to identify novel P-stalk binding proteins and to elucidate the general role of the P-stalk in translation. Using proximity labeling, we found that not only translational GTPase factors such as EF1A and EF2, but also translation initiation factors, aminoacyl-tRNA synthetase, RNA-binding proteins, ribosome-hibernation proteins and functionally unknown proteins localize near P-stalks. This finding is important as basic information for understanding of the multiple roles of P-stalks in translation.

Academic Significance and Societal Importance of the Research Achievements

遺伝情報の翻訳は最も基本的な細胞内プロセスの一つであり、その反応には多種多様な分子が関わる。本研究では、翻訳に必須である分子装置Pストークのはたらきを理解するための情報的基盤を構築した。本研究を足がかりとした「翻訳が滞りなく進行するための分子基盤」の解明により、(1)細胞システムの成り立ちの理解と、(2)翻訳の破綻が原因で生じる疾患の原因究明や治療法の確立において重要な知見が得られるようになると期待される。

Report

(4 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • Research Products

    (8 results)

All 2023 2022

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results,  Open Access: 3 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification2023

    • Author(s)
      Imai Hirotatsu、Utsumi Daisuke、Torihara Hidetsugu、Takahashi Kenzo、Kuroyanagi Hidehito、Yamashita Akio
    • Journal Title

      Nucleic Acids Research

      Volume: 51 Issue: 14 Pages: e76-e76

    • DOI

      10.1093/nar/gkad545

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification2023

    • Author(s)
      Imai Hirotatsu、Utsumi Daisuke、Torihara Hidetsugu、Takahashi Kenzo、Kuroyanagi Hidehito、Yamashita Akio
    • Journal Title

      bioRxiv

      Volume: - Pages: 1-33

    • DOI

      10.1101/2023.02.15.525786

    • Related Report
      2022 Research-status Report
    • Open Access
  • [Journal Article] Recruitment of translation factors by the tentacles of ribosomes2022

    • Author(s)
      今井 大達
    • Journal Title

      生化学

      Volume: 94 Issue: 5 Pages: 739-742

    • DOI

      10.14952/SEIKAGAKU.2022.940739

    • ISSN
      0037-1017
    • Year and Date
      2022-10-25
    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] The flexible N-terminal motif of uL11 unique to eukaryotic ribosomes interacts with P-complex and facilitates protein translation2022

    • Author(s)
      Yang Lei、Lee Ka-Ming、Yu Conny Wing-Heng、Imai Hirotatsu、Choi Andrew?Kwok-Ho、Banfield David?K、Ito Kosuke、Uchiumi Toshio、Wong Kam-Bo
    • Journal Title

      Nucleic Acids Research

      Volume: 50 Issue: 9 Pages: 5335-5348

    • DOI

      10.1093/nar/gkac292

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification2023

    • Author(s)
      Imai Hirotatsu、Utsumi Daisuke、Torihara Hidetsugu、Takahashi Kenzo、Kuroyanagi Hidehito、Yamashita Akio
    • Organizer
      RNA2023
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification2023

    • Author(s)
      今井大達、内海大介、鳥原英嗣、高橋健造、山下暁朗
    • Organizer
      第24回 日本RNA学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 小胞体ストレス応答におけるRNAの新規合成量と翻訳量の同時測定2023

    • Author(s)
      今井大達、内海大介、鳥原英嗣、高橋健造、山下暁朗
    • Organizer
      第46回 日本分子生物学学会年会
    • Related Report
      2023 Annual Research Report
  • [Presentation] High-speed AFM visualizes translational GTPase factor pool formed around the ribosomal P-stalk2022

    • Author(s)
      今井 大達、内海 利男、古寺 哲幸
    • Organizer
      第60回日本生物物理学会年会
    • Related Report
      2022 Research-status Report
    • Invited

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Published: 2021-04-28   Modified: 2025-01-30  

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