Identification of the specific signaling mediated by kinase and protease crosstalks in glioma stem cells using a unique integration method of phosphoproteomics and N-terminomics

• Project/Area Number: 21K15509
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: Kumamoto University
• Principal Investigator: CHANG CHIHHSIANG 熊本大学, 大学院生命科学研究部(医), 特別研究員 (40898805)
• Project Period (FY): 2021-04-01 – 2023-03-31
• Project Status: Granted (Fiscal Year 2021)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2022: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: phosphoproteomics / N-terminomics / proteomics / glioma stem cell

Outline of Research at the Start

Glioma stem cells (GSCs) are considered responsible for the therapeutic resistance and recurrence of malignant glioma. GSC clones having the potential to differentiate into malignant gliomas, were established and subjected to mass spectrometry(MS)-based proteomics in my present laboratory. We found that the kinases and proteases are uniquely regulated in GSCs during their differentiation. In this study, a unique integration of of phosphoproteomics and N-terminomics together with global proteomics, will be established and optimized for the crosstalk network screening in the clone of GSCs.