|Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2022: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2021: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|Outline of Research at the Start
Glioma stem cells (GSCs) are considered responsible for the therapeutic resistance and recurrence of malignant glioma. GSC clones having the potential to differentiate into malignant gliomas, were established and subjected to mass spectrometry(MS)-based proteomics in my present laboratory. We found that the kinases and proteases are uniquely regulated in GSCs during their differentiation. In this study, a unique integration of of phosphoproteomics and N-terminomics together with global proteomics, will be established and optimized for the crosstalk network screening in the clone of GSCs.