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Non-destructive measurement method that overcomes light scattering to determine the sex of chicken eggs in early incubation

Research Project

Project/Area Number 21K18228
Research Category

Grant-in-Aid for Challenging Research (Pioneering)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 41:Agricultural economics and rural sociology, agricultural engineering, and related fields
Research InstitutionKyoto University

Principal Investigator

Kondo Naoshi  京都大学, 農学研究科, 教授 (20183353)

Co-Investigator(Kenkyū-buntansha) 小川 雄一  京都大学, 農学研究科, 准教授 (20373285)
鈴木 哲仁  三重大学, 生物資源学研究科, 准教授 (00723115)
白神 慧一郎  京都大学, 農学研究科, 助教 (80795021)
Project Period (FY) 2021-07-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥25,870,000 (Direct Cost: ¥19,900,000、Indirect Cost: ¥5,970,000)
Fiscal Year 2023: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Keywords鶏卵 / 胚成長 / 非破壊計測 / レーザー / 透過画像 / 血管 / 画像解析 / 雌雄判定 / 散乱 / 非破壊検査
Outline of Research at the Start

本研究はこれまで黙認されてきた孵化直後の採卵鶏雄雛の70億羽殺処分という人類が抱える大きな未解決課題の解決を目的としている。そのために,雌雄と関係の深い鶏卵内の卵黄比率,性ホルモン変化,血液産生,心拍,胎動等の生物学的現象を,従来の透過分光だけでなく,微小な蛍光計測技術やレーザースペックル技術を活用した高度な非破壊計測技術を組み合わせた独創的手法によって情報収集する。この孵化前雌雄判定に成功すれば,経済的・エネルギ的問題解決のみならず,他の動物の受精卵の早期非破壊雌雄判定の可能性もうかがえる動物愛護・倫理に配慮した技術創出が期待される。

Outline of Final Research Achievements

This research aims to solve the major unsolved problem of killing 7 billion male egg-laying hens after hatching. For this purpose, we aimed to develop a sensing technology for biological phenomena in the hen's egg, which are closely related to the sex of the egg, not only by conventional transmission spectroscopy but also by advanced nondestructive measurement techniques utilizing image analysis and laser speckle technology. As a result of investigating the light transmissivity of eggshells and their relationship with hemoglobin, we showed that it is possible to visualize the egg embryo using transmission images at around 500 nm. The image analysis results showed that the area and fractal dimension of the embryo and blood vessels on the third day of egg hatching were significantly more prominent in males than in females. On the other hand, experiments showed that speckle images can also be used to measure embryonic growth through the eggshell.

Academic Significance and Societal Importance of the Research Achievements

鶏卵の非破壊雌雄判定法は,昨今のアニマルウェルフェアの観点からも極めて重要であり,世界的にも求められている技術である。鶏卵中の胚形成が孵化後の雌雄と関係があることは示唆されていたが,その成長に伴う血液量に着目した分光学的研究に留まっていた。本研究のように,鶏卵の光透過性を考慮し,胚成長を直接評価することで雌雄との関係を明らかにした例は少ない。また卵殻には点班が存在しており,その存在が透過画像の精度を低減させていることを明確にした。さらに,これを解決する方法としてスペックルイメージングが有効であることを見出し,特許出願を行っており,社会的意義も大きい研究であると言える。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Comments on the Screening Results   Research-status Report
  • Research Products

    (35 results)

All 2024 2023 2022 2021

All Journal Article (11 results) (of which Peer Reviewed: 4 results) Presentation (21 results) (of which Int'l Joint Research: 8 results,  Invited: 7 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] 蛍光特性に基づく魚の鮮度評価および農畜産分野への展開2024

    • Author(s)
      鈴木哲仁
    • Journal Title

      関西農業食料工学会報

      Volume: 135 Pages: 9-9

    • Related Report
      2023 Annual Research Report
  • [Journal Article] 孵卵中鶏胚の心拍信号における経験的モード分解の適用2024

    • Author(s)
      中島慶
    • Journal Title

      日本設備管理学会東海支部令和 5 年度学生研究発表会要旨集

      Volume: ー Pages: 17-18

    • Related Report
      2023 Annual Research Report
  • [Journal Article] 孵化予測のための鶏胚バイタルデータの経験的モード分解および特徴分析2024

    • Author(s)
      中島慶, 知原麻歩, 尾上魁, 小川雄一, 近藤直, 陳山鵬, 鈴木哲仁
    • Journal Title

      関西農業食料工学会報

      Volume: 136

    • Related Report
      2023 Annual Research Report
  • [Journal Article] 可視分光および透過画像による孵卵初期鶏胚の新規診断手法の可能性2023

    • Author(s)
      鈴木哲仁,知原麻歩,長岡伸一,尾上魁,田坂友彦,田村洋樹,小川雄一,近藤直
    • Journal Title

      農業環境工学関連学会2023年合同大会要旨集

      Volume: ー

    • Related Report
      2023 Annual Research Report
  • [Journal Article] Sexing of Chicken Egg in the Early Incubation Based on the Analysis of Transmission Image2023

    • Author(s)
      Maho Chihara, Shinichi Nagaoka, Akane Ogino, Hiroki Tamura, Tomohiko Tasaka, Tetsuhito Suzuki, Yuichi Ogawa, Keiichiro Shiraga, Naoshi Kondo
    • Journal Title

      Lecture Notes in Civil Engineering

      Volume: 458

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fluorescence Properties and Growth Conditions of Pseudomonas fluorescens in Chicken Egg2022

    • Author(s)
      Takato Ikebe, Tetsuhito Suzuki, Yuichi Ogawa, Keiichiro Shiraga, Naoshi Kondo
    • Journal Title

      Proceedings of CIGR The XX CIGR World Congress 2022

      Volume: ー Pages: 279-279

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Investigation of Scattering Property of Eggshell for Nondestructive Measurement of Chicken Egg2022

    • Author(s)
      Kazumasa Iwasaki, Yuichi Ogawa, Tetsuhito Suzuki, Keiichiro Shiraga, Naoshi Kondo
    • Journal Title

      Proceedings of CIGR The XX CIGR World Congress 2022

      Volume: ー Pages: 279-279

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gender Prediction of Hatching Chicken Eggs by Integrated Analysis of Visible Spectroscopy and Imaging2022

    • Author(s)
      Tetsuhito Suzuki, Maho Chihara, Shinichi Naogaoka, Tomohiko Tasaka, Yuchi Ogawa, Naoshi Kondo
    • Journal Title

      Proceedings of CIGR The XX CIGR World Congress 2022

      Volume: ー Pages: 280-280

    • Related Report
      2022 Research-status Report
    • Peer Reviewed
  • [Journal Article] 鶏卵の卵白濃度がPseudomonas fluorescensの増殖と蛍光特性に与える影響2022

    • Author(s)
      池邉貴、鈴木哲仁、樫森亜由子、白神慧一郎、小川雄一、近藤直
    • Journal Title

      関西農業食料工学会会報

      Volume: 131 Pages: 31-32

    • Related Report
      2021 Research-status Report
  • [Journal Article] 孵卵初期鶏卵の撮像系構築および透過画像解析2022

    • Author(s)
      知原麻歩、鈴木哲仁、樫森亜由子、白神慧一郎、小川雄一、近藤直
    • Journal Title

      関西農業食料工学会会報

      Volume: 131 Pages: 33-34

    • Related Report
      2021 Research-status Report
  • [Journal Article] 鶏卵におけるPseudomonas fluorescensの増殖条件と蛍光特性変化2021

    • Author(s)
      池邉貴、鈴木哲仁、樫森亜由子、白神慧一郎、小川雄一、近藤直
    • Journal Title

      関西農業食料工学会会報

      Volume: 130 Pages: 50-50

    • Related Report
      2021 Research-status Report
  • [Presentation] 孵卵中鶏胚の心拍信号における経験的モード分解の適用2024

    • Author(s)
      中島慶
    • Organizer
      日本設備管理学会東海支部学生研究発表会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 孵化予測のための鶏胚バイタルデータの経験的モード分解および特徴分析2024

    • Author(s)
      中島慶, 知原麻歩, 尾上魁, 小川雄一, 近藤直, 陳山鵬, 鈴木哲仁
    • Organizer
      関西農業食料工学会第151回例会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Sexing of Chicken Egg in the Early Incubation Based on the Analysis of Transmission Image2023

    • Author(s)
      Maho Chihara, Shinichi Nagaoka, Akane Ogino, Hiroki Tamura, Tomohiko Tasaka, Tetsuhito Suzuki, Yuichi Ogawa, Keiichiro Shiraga, Naoshi Kondo
    • Organizer
      15th International congress of Agricultural Mechanization and Energy in Agriculture
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛍光特性に基づく魚の鮮度評価および農畜産分野への展開2023

    • Author(s)
      鈴木哲仁
    • Organizer
      関西農業食料工学会第150回例会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 可視分光および透過画像による孵卵初期鶏胚の新規診断手法の可能性2023

    • Author(s)
      鈴木哲仁,知原麻歩,長岡伸一,尾上魁,田坂友彦,田村洋樹,小川雄一,近藤直
    • Organizer
      農業環境工学関連学会2023年合同大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Innovations in protected cultivation: Automation and robotics for crop production-Start with Fluorescence Sensing for Solving the Trade-off Problem “Food-Environment”2023

    • Author(s)
      Naoshi Kondo
    • Organizer
      Global Conference on Sustainable Agricultural Mechanization (GAMC)
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Smart Livestock for sustainable animal production with higher welfare2023

    • Author(s)
      Naoshi Kondo
    • Organizer
      the 7th World Intelligence Congress Intelligent Agriculture Summit Forum & International Conference on Intelligent Agriculture (ICIA 2023)
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] スマート農食産業に貢献する光センシング2023

    • Author(s)
      近藤 直
    • Organizer
      (一社)電子情報通信学会 光通信システム研究専門委員会,第37回光通信システムシンポジウム「国際社会を支える光通信技術が向かう先~新しい社会に期待されるイノベーション~」
    • Related Report
      2023 Annual Research Report
    • Invited
  • [Presentation] Digital Transformation for Sustainable Asian Small Scale Intensive Agriculture2023

    • Author(s)
      Naoshi Kondo
    • Organizer
      International Joint Workshop on DX Research and Education to Realize Sustainable Food Production
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Light & sound-based sensing technologies for products of agriculture, livestock and aquaculture2023

    • Author(s)
      Tetsuhito Suzuki
    • Organizer
      International Webinar of "Technology Innovation Towards Smart and Sustainable Future
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Fluorescence Properties and Growth Conditions of Pseudomonas fluorescens in Chicken Egg2022

    • Author(s)
      Takato Ikebe、Tetsuhito Suzuki、Yuichi Ogawa、Keiishiro Shiraga、Naoshi Kondo
    • Organizer
      CIGR 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Investigation of Scattering Property of Eggshell for Nondestructive Measurement of Chicken Egg2022

    • Author(s)
      Kazumasa Iwasaki、Yuichi Ogawa、Tetsuhito Suzuki、Keiishiro Shiraga、Naoshi Kondo
    • Organizer
      CIGR 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Gender Prediction of Hatching Chicken Eggs by Integrated Analysis of Visible Spectroscopy and Imaging2022

    • Author(s)
      Tetsuhito Suzuki、Maho Chihara、Shin-ichi Nagaoka、Tomohiko Tasaka、Yuichi Ogawa、Naoshi Kondo
    • Organizer
      CIGR 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Super Precision Agriculture to Food Tech for Consumers' Diversified Preference and Sustainable Agriculture2022

    • Author(s)
      Naoshi Kondo
    • Organizer
      ISMAB 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Collaboration with Companies and Governmental Research Institutes for Modernization of Agriculture2022

    • Author(s)
      Naoshi Kondo
    • Organizer
      ISMAB 2022
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 孵卵初期鶏卵の可視透過画像および分光分析による雌雄判別2022

    • Author(s)
      知原麻歩、鈴木哲仁、樫森亜由子、長岡伸一、白神慧一郎、小川雄一、近藤直
    • Organizer
      関西農業食料工学会 第147回例会
    • Related Report
      2021 Research-status Report
  • [Presentation] 高効率・高品質な雛生産のための孵卵中鶏卵の分光及び画像測定装置の構築2021

    • Author(s)
      鈴木哲仁、知原麻歩、樫森亜由子、小川雄一、近藤直
    • Organizer
      農業食料工学会 第79回年次大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 鶏卵の卵白濃度がPseudomonas fluorescensの増殖と蛍光特性に与える影響2021

    • Author(s)
      池邉貴、鈴木哲仁、樫森亜由子、白神慧一郎、小川雄一、近藤直
    • Organizer
      関西農業食料工学会 第146回例会
    • Related Report
      2021 Research-status Report
  • [Presentation] 孵卵初期鶏卵の撮像系構築および透過画像解析2021

    • Author(s)
      知原麻歩、鈴木哲仁、樫森亜由子、白神慧一郎、小川雄一、近藤直
    • Organizer
      関西農業食料工学会 第146回例会
    • Related Report
      2021 Research-status Report
  • [Presentation] A Consideration for Sustainable LivestockFarming2021

    • Author(s)
      Naoshi Kondo
    • Organizer
      農業食料工学会 第79回年次大会
    • Related Report
      2021 Research-status Report
  • [Presentation] Optical Property of Eggshell from Younger and Older Hens in Teraheltz Region2021

    • Author(s)
      Alin Khalidduzzaman、Keiichiro Shiraga、Tetsuhito Suzuki、Naoshi Kondo、Yuichi Ogawa
    • Organizer
      農業食料工学会 第79回年次大会
    • Related Report
      2021 Research-status Report
  • [Patent(Industrial Property Rights)] 鶏卵状態判定装置2024

    • Inventor(s)
      小川雄一,知原麻歩,田村洋樹,尾上魁
    • Industrial Property Rights Holder
      小川雄一,知原麻歩,田村洋樹,尾上魁
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2024-023092
    • Filing Date
      2024
    • Related Report
      2023 Annual Research Report
  • [Patent(Industrial Property Rights)] 種卵の非破壊検査装置及び種卵の非破壊検査方法2022

    • Inventor(s)
      小川雄一,近藤直,鈴木哲仁
    • Industrial Property Rights Holder
      小川雄一,近藤直,鈴木哲仁
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-115339
    • Filing Date
      2022
    • Related Report
      2022 Research-status Report
  • [Patent(Industrial Property Rights)] 種卵の非破壊検査装置及びそれに用いる種卵検査プログラム2022

    • Inventor(s)
      鈴木哲仁、知原麻歩、近藤直、小川雄一、田原孝嗣
    • Industrial Property Rights Holder
      京都大学,ナベル
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-028941
    • Filing Date
      2022
    • Related Report
      2021 Research-status Report

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Published: 2021-07-13   Modified: 2025-01-30  

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