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Elucidation of holobiont co-evolution mechanism by analyzing aquatic plant-surface symbiotic bacteria interactions

Research Project

Project/Area Number 21K18239
Research Category

Grant-in-Aid for Challenging Research (Pioneering)

Allocation TypeMulti-year Fund
Review Section Medium-sized Section 45:Biology at organismal to population levels and anthropology, and related fields
Research InstitutionHokkaido University

Principal Investigator

Morikawa Masaaki  北海道大学, 地球環境科学研究院, 教授 (20230104)

Co-Investigator(Kenkyū-buntansha) 小山 時隆  京都大学, 理学研究科, 准教授 (30324396)
Project Period (FY) 2021-07-09 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥26,000,000 (Direct Cost: ¥20,000,000、Indirect Cost: ¥6,000,000)
Fiscal Year 2023: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2022: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2021: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
Keywordsホロビオント / 共生 / ウキクサ / 植物成長促進細菌 / 相互作用分子機構 / 共生微生物 / 表層細菌 / 相利共生 / 植物成長促進因子 / 共進化 / 植物ー微生物共生
Outline of Research at the Start

土壌環境とは大きく異なり、分子が拡散しやすい水圏環境における、宿主植物と表層共生細菌の特有の相利共生と共進化の世界を探る。具体的には、ウキクサ亜科植物を対象として、共生細菌が生産する水生植物成長促進因子とその生合成遺伝子群の構造ー機能相関について、微生物学的研究と植物生理学的研究の両面から総合的に取り組む。さらに、これまでに構築した、無菌ウキクサをプラットフォームとした複数種共生細菌の協調あるいは拮抗と、宿主植物の成長を定量的に測定するシステムを活用して、未開拓領域である水生植物微生物共生総体(ホロビオント)の生存戦略の一端を分子レベルで理解し、生物共進化の新たな側面に光を当てる。

Outline of Final Research Achievements

In this research project, we focused on the microbial symbiosis of freshwater aquatic plants, and investigated the interaction molecular mechanisms between host plants and surface symbiotic microorganisms from both plant physiological and microbiological studies. Symbiotic bacteria of the genus Acinetobacter and Pseudomonas were shown to produce exopolysaccharides and lipopolysaccharides with different structures, so that they attach to different locations on the host plant and promote its growth. On the other hand, within a plant body, the growth was found to be promoted through the following responses; bacterial adhesion > increased iron utilization efficiency > promotion of nitrogen and chlorophyll metabolism > chloroplast development > increased photosynthetic activity. Furthermore, we discovered that surface symbiotic bacteria of the genus Bacillus promote the growth of host plants through secreting extracellular peptidases.

Academic Significance and Societal Importance of the Research Achievements

本研究成果はこれまで未解明であった、淡水水生植物と表層共生微生物による成長促進作用について双方の研究からその分子機構を明らかにした。これは地球生物圏を支える仕組みの一部を理解するものであると共に両者のホロビオントとしての共進化を理解する手がかりを提供する。一方、研究の対象とした淡水水生植物ウキクサは光合成による廃水浄化作用を有し、増殖した植物バイオマスは資源価値が高いことが指摘されているため、ここで得られた知見は省エネ水処理技術および有用バイオマス生産技術にも利用可能であることから、資源循環型低炭素社会基盤の構築にも寄与する。

Report

(3 results)
  • 2023 Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Comments on the Screening Results
  • Research Products

    (16 results)

All 2023 2022 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] カセサート大学/マヒドン大学/コンケン大学(タイ)

    • Related Report
      2022 Research-status Report
  • [Journal Article] Spontaneous Cell Lysis by <i>Pelomonas saccharophila</i> MRB3 Provides Plant-Available Macronutrients in Hydroponic Growth Media and Accelerates Biomass Production of Duckweed2023

    • Author(s)
      Ishizawa Hidehiro、Kaji Yukiko、Shimizu Yuki、Kuroda Masashi、Inoue Daisuke、Makino Ayaka、Nakai Ryosuke、Tamaki Hideyuki、Morikawa Masaaki、Ike Michihiko
    • Journal Title

      Journal of Water and Environment Technology

      Volume: 21 Issue: 1 Pages: 49-58

    • DOI

      10.2965/jwet.22-054

    • ISSN
      1348-2165
    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Diazotrophic bacterium Azotobacter vinelandii as a mutualistic growth promoter of an aquatic plant: Lemna minor2022

    • Author(s)
      Shuvro Sajjad Kamal、Jog Rahul、Morikawa Masaaki
    • Journal Title

      Plant Growth Regulation

      Volume: 100 Issue: 1 Pages: 171-180

    • DOI

      10.1007/s10725-022-00948-0

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Growth Promotion of Giant Duckweed Spirodela polyrhiza (Lemnaceae) by Ensifer sp. SP4 Through Enhancement of Nitrogen Metabolism and Photosynthesis2022

    • Author(s)
      Toyama Tadashi、Mori Kazuhiro、Tanaka Yasuhiro、Ike Michihiko、Morikawa Masaaki
    • Journal Title

      Molecular Plant-Microbe Interactions

      Volume: 35 Issue: 1 Pages: 28-38

    • DOI

      10.1094/mpmi-06-21-0157-r

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Circadian-period variation underlies the local adaptation of photoperiodism in the short-day plant Lemna aequinoctialis2022

    • Author(s)
      Muranaka Tomoaki、Ito Shogo、Kudoh Hiroshi、Oyama Tokitaka
    • Journal Title

      iScience

      Volume: 25 Issue: 7 Pages: 104634-104634

    • DOI

      10.1016/j.isci.2022.104634

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Siderophore for Lanthanide and Iron Uptake for Methylotrophy and Plant Growth Promotion in Methylobacterium aquaticum Strain 22A2022

    • Author(s)
      Juma Patrick Otieno、Fujitani Yoshiko、Alessa Ola、Oyama Tokitaka、Yurimoto Hiroya、Sakai Yasuyoshi、Tani Akio
    • Journal Title

      Frontiers in Microbiology

      Volume: 13 Pages: 921635-921635

    • DOI

      10.3389/fmicb.2022.921635

    • Related Report
      2022 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Dual function of environmental bacteria that enable duckweed prosperity2022

    • Author(s)
      Masaaki Morikawa
    • Organizer
      6th International Conference on Duckweed Research and Applications
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] A soil bacterium Azotobacter vinelandii contributes to growth promotion of duckweed through nitrogen fixation, bacterial synergism and EPS production2022

    • Author(s)
      Sajjad Kamal, Rahul Jog, Masaaki Morikawa
    • Organizer
      6th International Conference on Duckweed Research and Applications
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research
  • [Presentation] Variability of chronobiological characteristics in duckweed2022

    • Author(s)
      Tokitaka Oyama
    • Organizer
      6th International Conference on Duckweed Research and Applications
    • Related Report
      2022 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Spontaneous cell Lysis by Pelomonas saccharophila MRB3 provides plant-available macronutrients in hydroponic growth media and accelerates biomass production of duckweed2022

    • Author(s)
      Hidehiro Ishizawa, Yukiko Kaji, Yuki Shimizu, Masashi Kuroda, Daisuke Inoue, Ayaka Makino, Ryosuke Nakai, Hideyuki Tamaki, Masaaki Morikawa, Michihiko Ike
    • Organizer
      日本水環境学会年会2022年度大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 微細藻類との栄養競合を回避しつつウキクサの成長を担保する環境細菌2022

    • Author(s)
      Yeni Khairina, Rahul Jog, 森川正章
    • Organizer
      環境バイオテクノロジー学会2022年度大会
    • Related Report
      2022 Research-status Report
  • [Presentation] Reconstruction of a duckweed holobiont that reduces nutrient competition with microalgae2022

    • Author(s)
      Huyen Thi Thanh Pham, Masaaki Morikawa
    • Organizer
      環境バイオテクノロジー学会2022年度大会
    • Related Report
      2022 Research-status Report
  • [Presentation] ウキクサ-微生物共培養法により分離培養した新規細菌捕食性細菌の性質2022

    • Author(s)
      森下陽介、田中靖浩、山村英樹、菅野 学、玉木秀幸、鎌形洋一、森川正章、遠山 忠、森 一博
    • Organizer
      微生物生態学会2022年度大会
    • Related Report
      2022 Research-status Report
  • [Presentation] 水生植物ウキクサの成長を促進する新規Verrucomicrobiota門細菌 hF2-5株の系統と生理特性2022

    • Author(s)
      牧野 彩花、中井 亮佑、田中靖浩、森川 正章、鎌形 洋一、玉木秀幸
    • Organizer
      微生物生態学会2022年度大会
    • Related Report
      2022 Research-status Report
  • [Book] Design of Materials and Technologies for Environmental Remediation2023

    • Author(s)
      Shunitz Tanaka, Masaaki Kurasaki, Masaaki Morikawa, Yuichi Kamiya
    • Total Pages
      681
    • Publisher
      Springer
    • ISBN
      9789811952357
    • Related Report
      2022 Research-status Report
  • [Remarks] SATREPS タイ国・生物循環グリーン経済実現に向けたウキクサホロビオント資源価値の包括的開拓

    • URL

      https://www.jst.go.jp/global/kadai/r0204_thailand.html

    • Related Report
      2022 Research-status Report

URL: 

Published: 2021-07-13   Modified: 2025-01-30  

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