|Budget Amount *help
¥217,230,000 (Direct Cost: ¥167,100,000、Indirect Cost: ¥50,130,000)
Fiscal Year 2014: ¥32,760,000 (Direct Cost: ¥25,200,000、Indirect Cost: ¥7,560,000)
Fiscal Year 2013: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2012: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2011: ¥52,910,000 (Direct Cost: ¥40,700,000、Indirect Cost: ¥12,210,000)
Fiscal Year 2010: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
|Outline of Final Research Achievements
In this project, we studied structure and function of water channels, ion channels, gap junction channels, acetylcholine receptor, claudins and H+,K+-ATPase, because these membrane proteins are important components in biological systems. We could obtain significant results in these research subjects by mainly electron and X-ray crystallography.
For example, we intensively studied adhennels, channels with adhesive function. Claudin is an adhennel family protein and a key molecule in tight junctions. We analyzed structure of claudin-15 and proposed a paracellular channel model to explain channel function through epithelial cell sheets. We also analyzed the complex structure of claudin-19 and the C-terminal domain of Clostridium perfringens enterotoxin, which causes disintegration of tight junctions. These results are of great interest for both basic biology and the rational design of therapeutic agents that can potentially be used to deliver drugs across tissue barriers.