Generating molecular diversity by the wide range of polyketide synthases
| Project/Area Number |
22241054
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Living organism molecular science
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
久城 哲夫 明治大学, 農学部, 准教授 (80373299)
梅原 薫 静岡県立大学, 薬学部, 講師 (40185070)
渡辺 賢二 静岡県立大学, 薬学部, 准教授 (50360938)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
渡辺 賢二 静岡県立大学, 薬学部, 准教授 (50360938)
飯田 滋 静岡県立大学, 生活健康科学研究科・薬学研究科, 特任教授 (30012777)
|
|---|
| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2012: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2011: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2010: ¥20,540,000 (Direct Cost: ¥15,800,000、Indirect Cost: ¥4,740,000)
|
|---|
| Keywords | ゲノム / 酵素工学 / 生理活性 / biosynthesis / 生合成 / メロテルペノイド / 分子多様性 / テルペン環化酵素 / 酵素 / テレトニン / プレニル基転移酵素 / バイオテクノロジー |
|---|
| Research Abstract |
Novel type III PKS was isolated from Garcinia mangostana L. , which contains mainly prenylated xanthones. A key prenyltransferase involved in the biosynthesis of abundant fungal meroterpenoids derived from 3,5-dimethylorsellinic was identified. The prenylated product serves as a key intermediate in the biosynthesis of series of meroterpenoids in fungi. With Aspergillus nidlans, Metabolic engineering of yeast for heterologous production of tryprostatins and other compounds was developed through our engineered strain of Saccharomyces cerevisiae, SCKW5. Further trial to architect comprehensive system to form secondary metabolites with Chaetomium globosum is under progress.
|
Report
(4 results)
Research Products
(44 results)
-
-
-
-
-
-
-
-
-
[Journal Article] Establishing a new methodology for genome mining and biosynthesis of polyketides and peptides through yeast molecular genetics2012
Author(s)
K. Ishiuchi, T. Nakazawa, T. Ookuma, S. Sugimoto, M. Sato, Y. Tsunematsu, N. Ishikawa, H. Noguchi, K. Hotta, H. Moriya, K. Watanabe
-
Journal Title
ChemBioChem
Volume: 13(6)
Pages: 846-854
Related Report
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Overexpressing transcriptional regulator in Aspergillus oryzae activates a silent biosynthetic pathway to produce novel polyketide2012
Author(s)
Nakazawa, T., Ishiuchi, K., Praseuth, A., Noguchi, H., Hotta, K., Moriya, H., Watanabe, K.
-
Journal Title
ChemBioChem
Volume: 13
Pages: 855-861
Related Report
Peer Reviewed
-
[Journal Article] Establishing a new methodology for genome mining and biosynthesis of polyketides and peptides through yeast molecular genetics2012
Author(s)
Ishiuchi, K., Nakazawa, T., Ookuma, T., Sugimoto, T., Sato, M., Tsunematsu, Y., Ishikawa, N., Noguchi, H., Hotta, K., Moriya, H., Watanabe, K.
-
Journal Title
ChemBioChem
Volume: 13
Pages: 846-854
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] A comprehensive overview on genomically directed assembly of aromatic polyketides and macrolide lactones using fungal megasynthases2011
Author(s)
Saruwatari, T., Praseuth, A.P., Sato, M., Torikai, K., Noguchi, H., Watanabe, K.
-
Journal Title
J.Antibiot.
Volume: 64
Pages: 9-17
NAID
Related Report
Peer Reviewed
-
[Journal Article] Practical synthesis of DOPA derivative for biosynthetic production of potent antitumor natural products, saframycins and ecteinascidin 7432011
Author(s)
Torikai, K., Saruwatari, T., Kitano, T., Sano, T., Nakane, A., Noguchi, H., Watanabe, K.
-
Journal Title
Letters In Organic Chemistry
Volume: 8
Pages: 686-689
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] T. Nakazawa, K. Ishiuchi, K. Hotta, H.Noguchi, H. Moriya, K. Watanabe2011
Author(s)
T. Nakazawa, K. Ishiuchi, K. Hotta, H.Noguchi, H. Moriya, K. Watanabe
Organizer
8th AFMC International Medicinal Chemistry Symposium "Frontier of Medicinal Science"
Place of Presentation
KEIO PLAZA HOTEL TOKYO(東京)
Year and Date
2011-11-29
Related Report
-
-
-
-
-
