| Budget Amount *help |
¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2014: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
In general, vibrational spectroscopy has never been recognized as one of structural biology tools, but we have shown that light-induced difference FTIR spectroscopy is a powerful tool for the analysis of structural changes in photoreceptive proteins for function. This project aims at establishing difference FTIR spectroscopy as a tool of similar analysis for non-photoreceptive membrane proteins. We optimized the experimental setup using attenuated total reflection (ATR) FTIR spectroscopy, which enabled to obtain difference FTIR spectra upon various stimuli in solution. We obtained structural information of ion-channels (potassium, sodium, proton and water channels), ion-pumps that specifically bind sodium and chloride ions, rotary motors such as V-type ATPase, GPCRs such as taste-receptor, and transporters such as POT. We reported more than 70 articles in Nature, Nature Commun., Nature Nanotech., JACS, Angew., PNAS, JPC Letters and JBC during the project period.
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