Molecular mechanisms of the polarity-regulating signaling

• Project/Area Number: 22247030
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Yokohama City University
• Principal Investigator: OHNO Shigeo 横浜市立大学, 医学研究科, 教授 (10142027)
• Co-Investigator(Renkei-kenkyūsha): HIRAI Syu-ichi 和歌山県立医科大学, 医学部, 教授 (80228759) ; SUZUKI Atsushi 横浜市立大学, 医学研究科, 准教授 (00264606) ; AKIMOTO Kazunori 東京理科大学, 薬学部生命創薬科学科, 准教授 (70285104) ; YAMASHITA Akio 横浜市立大学, 医学部, 講師 (20405020) ; HIROSE Tomonori 横浜市立大学, 医学部, 助教 (20381668) ; NAKAYA Masa-aki 横浜市立大学, 医学部, 助教 (70422095) ; SASAKI Kazunori 横浜市立大学, 医学部, 助教 (50438131)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000); Fiscal Year 2012: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000); Fiscal Year 2011: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000); Fiscal Year 2010: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
• Keywords: 癌 / 再生医学 / 細胞 / 組織 / シグナル伝達 / 発生 / 分化 / 細胞・組織 / 発生・分化 / 発生・文化

Research Abstract

To understand the molecular mechanism of the PAR-aPKC polarity complex to regulate cell polarity, we identified ASPP2 as a novel member of the PAR-aPKC polarity complex. ASPP2 as well as PAR3 localizes to the apical junctional complex of epithelial cells and their localization are mutually-dependent.Another finding involves a novel regulatory system for the exocytosis of apical membrane proteins by the PAR-aPKC polarity complex. We identified KIBRA as a competitive inhibitor of aPKC kinase activity. Further studies revealed that KIBRA suppress apical exocytosis through inhibition of the aPKC kinase activity.

Research Products

• [Journal Article] The interaction of Kinesin-1 with its adaptor protein JIP1 can be regulated via proteins binding to the JIP1-PTB domain. (2013)
  • Author(s): Satake T, et al.
  • Journal Title: BMC Cell Biol Volume: 14 Issue: 1 Pages: 12-12
  • DOI: 10.1186/1471-2121-14-12
  • Peer Reviewed
• [Journal Article] Spatial regulation of VEGF receptor endocytosis in angiogenesis (2013)
  • Author(s): Nakayama, M.
  • Journal Title: Nat Cell Biol Volume: 15 Issue: 3 Pages: 249-60
  • DOI: 10.1038/ncb2679
  • Peer Reviewed
• [Journal Article] High expression of KIBRA in low atypical protein kinase C-expressing gastric cancer correlates with lymphatic invasion and poor prognosis (2013)
  • Author(s): Y. Yoshihama, Y. Izumisawa, K. Akimoto, Y. Satoh, T. Mizushima, K. Satoh, K. Chida, R. Takagawa, H. Akiyama, Y. Ichikawa, C. Kunisaki, Y. Inayama, I. Endo, Y. Nagashima and S. Ohno
  • Journal Title: Cancer Sci Volume: 104 Issue: 2 Pages: 259-65
  • DOI: 10.1111/cas.12066
  • Peer Reviewed
• [Journal Article] Tumor type-dependent function of the par3 polarity protein in skin tumorigenesis (2012)
  • Author(s): S. Iden, W. E. van Riel, R. Schafer, J. Y. Song, T. Hirose, S. Ohno and J. G. Collard
  • Journal Title: Cancer Cell Volume: 22 Issue: 3 Pages: 389-403
  • DOI: 10.1016/j.ccr.2012.08.004
  • Peer Reviewed
• [Journal Article] he KIBRA-aPKC connection: A potential regulator of membrane trafficking and cell polarity (2012)
  • Author(s): Y. Yoshihama, K. Chida and S. Ohno
  • Journal Title: Commun Integr Biol Volume: 5 Issue: 2 Pages: 146-51
  • DOI: 10.4161/cib.18849
  • Peer Reviewed
• [Journal Article] PAR-1/MARK: a kinase essential for maintaining the dynamic state of microtubules (2012)
  • Author(s): Hayashi K, Suzuki A and Ohno S
  • Journal Title: Cell Struct Funct Volume: 37 Pages: 21-5
  • NAID: 130004053887
  • URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22139392
  • Peer Reviewed
• [Journal Article] Heat shock protein 90 regulates phosphatidylinositol 3-kinase-related protein kinase family proteins together with the RUVBL1/2 and Tel2-containing co-factor complex (2012)
  • Author(s): Izumi N, Yamashita A, Hirano H, Ohno S
  • Journal Title: Cancer Sci Volume: 103 Issue: 1 Pages: 50-57
  • DOI: 10.1111/j.1349-7006.2011.02112.x
  • Peer Reviewed
• [Journal Article] Integrated regulation of PIKK-mediated stress responses by AAA+ proteins RUVBL1 and RUVBL2 (2012)
  • Author(s): Izumi N, Yamashita A and Ohno S
  • Journal Title: Nucleus Volume: 3 Issue: 1 Pages: 29-43
  • DOI: 10.4161/nucl.18926
  • Peer Reviewed
• [Journal Article] Regulation of the alternative splicing of sarcoplasmic reticulum Ca^<2+>-AT Pase1 (SERCA1) by phorbol 12-myristate 13-acetate (PMA) via a PKC pathway. (2012)
  • Author(s): Zhao Y, Koebis M, Suo S, Ohno S, Ishiura S.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 423 Issue: 2 Pages: 212-217
  • DOI: 10.1016/j.bbrc.2012.05.033
  • Peer Reviewed
• [Journal Article] Analysis of interferon-beta mRNA stability control after poly(I:C) stimulation using RNA metabolic labeling by ethynyluridine. (2012)
  • Author(s): Abe K, Ishigami T, Shyu A. B, Ohno S, Umemura S, Yamashita A
  • Journal Title: Biochem Biophys Res Commun Volume: 428(1) Issue: 1 Pages: 44-49
  • DOI: 10.1016/j.bbrc.2012.09.144
  • Peer Reviewed
• [Journal Article] Integrated regulation of PIKK-mediated stress responses by AAA+ proteins RUVBL1 and RUVBL2. (2012)
  • Author(s): Izumi N, Yamashita A, Ohno S
  • Journal Title: Nucleus Volume: 3(1) Pages: 29-43
  • Peer Reviewed
• [Journal Article] N- and C-terminal Upf1 phosphorylations create binding platforms for SMG-6 and SMG-5:SMG-7 during NMD (2012)
  • Author(s): Okada-Katsuhata Y, Yamashita A, Kutsuzawa K, Izumi N, Hirahara F, Ohno S
  • Journal Title: Nucleic Acids Res Volume: 40 Issue: 3 Pages: 1251-1266
  • DOI: 10.1093/nar/gkr791
  • Peer Reviewed
• [Journal Article] PAR-1/MARK: a kinase essential for maintaining the dynamic state of microtubules. (2012)
  • Author(s): Hayashi K, Suzuki A, Ohno S
  • Journal Title: Cell Struct Funct Volume: 37(1) Pages: 21-25
  • NAID: 130004053887
  • Peer Reviewed
• [Journal Article] Maintenance of dendritic spine morphology by partitioning-defective 1b through regulation of microtubule growth (2011)
  • Author(s): Hayashi K, Suzuki A, Hirai S, Kurihara Y, Hoogenraad CC, Ohno S
  • Journal Title: J. Neurosci. Volume: 31 Issue: 34 Pages: 12094-12103
  • DOI: 10.1523/jneurosci.0751-11.2011
  • Peer Reviewed
• [Journal Article] The co-expression of aPKCλ/ι and IL-6 in prostate cancer tissue correlates with biochemical recurrence (2011)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kagawa E, Sasaki T, Sano J, Takagawa R, Fujinami K, Sasaki K, Aoki I, Ohno S, Kubota Y and Uemura H
  • Journal Title: Cancer Science Volume: 102 Issue: 8 Pages: 1576-1581
  • DOI: 10.1111/j.1349-7006.2011.01972.x
  • NAID: 10030242512
  • Peer Reviewed
• [Journal Article] Phosphoinositide binding by par-3 involved in par-3 localization (2011)
  • Author(s): Horikoshi Y, Hamada S, Ohno S and Suetsugu S:
  • Journal Title: Cell Structure and Function Volume: 36 Pages: 97-102
  • NAID: 40019163941
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21467691
  • Peer Reviewed
• [Journal Article] Axon formation in neocortical neurons depends on stage-specific regulation of microtubule stability by the DLK-JNK pathway (2011)
  • Author(s): Hirai S-I, Banba Y, Satake T and Ohno S:
  • Journal Title: J. Neuroscience Volume: 3 Issue: 17 Pages: 6468-6480
  • DOI: 10.1523/jneurosci.5038-10.2011
  • Peer Reviewed
• [Journal Article] KIBRA suppresses apical exocytosis through inhibition of aPKC activity in epithelial cells (2011)
  • Author(s): Yohei Yoshihama
  • Journal Title: Current Biology Volume: 21 Issue: 8 Pages: 705-711
  • DOI: 10.1016/j.cub.2011.03.029
  • Peer Reviewed
• [Journal Article] KIBRA Suppresses Apical Exocytosis through Inhibition of aPKC Kinase Activity in Epithelial Cells. (2011)
  • Author(s): Yoshihama Y, Sasaki K , Horikoshi Y, Suzuki A, Ohtsuka T, Hakuno F, Takahashi S-I, Ohno S, Chida K
  • Journal Title: Current Biol. Volume: 21(8) Pages: 705-711
  • Peer Reviewed
• [Journal Article] Axon formation in neocortical neurons depends on stage-specific regulation of microtubule stability by the DLK-JNK pathway. (2011)
  • Author(s): Hirai S-I, Banba Y, Satake T, Ohno S
  • Journal Title: J. Neuroscience Volume: 31(17) Pages: 6468-6480
  • Peer Reviewed
• [Journal Article] Phosphoinositide binding by par-3 involved in par-3 localization. (2011)
  • Author(s): Horikoshi Y, Hamada S, Ohno S, Suetsugu S
  • Journal Title: Cell Structure and Function Volume: 36(1) Pages: 97-102
  • NAID: 40019163941
  • Peer Reviewed
• [Journal Article] The co-expression of aPKCλ/ι and IL-6 in prostate cancer tissue correlates with biochemical recurrence. (2011)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kagawa E, Sasaki T, Sano J, Takagawa R, Fujinami K, Sasaki K, Aoki I, Ohno S, Kubota Y, Uemura H
  • Journal Title: Cancer Science Volume: 102(8) Pages: 1576-1581
  • NAID: 10030242512
  • Peer Reviewed
• [Journal Article] Maintenance of dendritic spine morphology by partitioning-defective 1b through regulation of microtubule growth. (2011)
  • Author(s): Hayashi K, Suzuki A, Hirai S, Kurihara Y, Hoogenraad CC, Ohno S
  • Journal Title: J Neurosci Volume: 31(34) Pages: 12094-12103
  • Peer Reviewed
• [Journal Article] A novel role for hSMG-1 in stress granule formation. (2011)
  • Author(s): Brown J A, Roberts T L, Richards R, Woods R, Birrell G, Lim YC, Ohno S, Yamashita A, Abraham RT, Gueven N, Lavin MF
  • Journal Title: Mol Cell Biol Volume: 31(22) Pages: 4417-4429
  • Peer Reviewed
• [Journal Article] Axon Formation in Neocortical Neurons Depends on Stage-Specific Regulation of Microtubule Stability by the Dual Leucine Zipper Kinase-c-Jun N-terminal Kinase Pathway. (2011)
  • Author(s): Hirai S, Banba Y, Satake T, Ohno S
  • Journal Title: J Neuroscience Volume: 31(17) Pages: 6468-6480
  • Peer Reviewed
• [Journal Article] KIBRA Suppresses Apical Exocytosis through Inhibition of aPKC Kinase Activity in Epithelial Cells. (2011)
  • Author(s): Yoshihama Y, Sasaki K, Horikoshi Y, Suzuki A, Ohtsuka T, Hakuno F, Takahashi S-I, Ohno S, Chida K
  • Journal Title: Current Biol Volume: 21 Pages: 1-7
  • Peer Reviewed
• [Journal Article] The nonsense-mediated mRNA decay SMG-1 kinase is regulated by large-scale conformational changes controlled by SMG-8. (2011)
  • Author(s): Arias-Palomo E, Yamashita A, Fernandez IS, Nunez-Ramirez R, Bamba Y, Izumi N, Ohno S, Llorca O.
  • Journal Title: Genes Dev. Volume: 25(2) Pages: 153-164
  • Peer Reviewed
• [Journal Article] Characterization of SMG-9, an essential component of the nonsense-mediated mRNA decay. SMG1C complex. (2011)
  • Author(s): Fernandez IS, Yamashita A, Arias-Palomo E, Bamba Y, Bartolome RA, Canales MA, Teixido J, Ohno S, Llorca O
  • Journal Title: Nucleic Acids Res. Volume: 39(1) Pages: 347-358
  • Peer Reviewed
• [Journal Article] AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay (2010)
  • Author(s): Izumi N, Yamashita A, Iwamatsu A, Kurata R, Nakamura H, Saari B, HiranoH, Anderson P and Ohno S
  • Journal Title: Sci Signal Volume: 116 Issue: 116 Pages: 27-27
  • DOI: 10.1126/scisignal.2000468
  • Peer Reviewed
• [Journal Article] ASPP2 regulates epithelial cell polarity through the PAR complex (2010)
  • Author(s): Cong W, Hirose T, Harita Y, Yamashita A, Mizuno K, Hirano H and Ohno S
  • Journal Title: Curr Biol Volume: 20 Pages: 1408-14
  • Peer Reviewed
• [Journal Article] ASPP2 regulates epithelial cell polarity through the PAR complex. (2010)
  • Author(s): Cong W, Hirose T, Harita Y, Yamashita A, Mizuno K, Hirano H, Ohno S
  • Journal Title: Curr.Biol. Volume: 20(15) Pages: 1408-1414
  • Peer Reviewed
• [Journal Article] AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK family and function in nonsense-mediated mRNA decay. (2010)
  • Author(s): Izumi N, Yamashita A, Iwamatsu A, Kurata R, Nakamura H, Saari B, Hirano H, Anderson P, Ohno S
  • Journal Title: Science Signaling Volume: 3(116)
  • Peer Reviewed
• [Journal Article] Analysis of Nonsense-Mediated mRNA Decay by Monitoring mRNA Half-Lives in Mammalian Cells. (2010)
  • Author(s): Yamashita A, Ohno S
  • Journal Title: Cold Spring Harbor Protocols Volume: (2)
  • Peer Reviewed
• [Journal Article] The 8th and 9th tandem spectrin-like repeats of utrophin cooperatively form a functional unit to interact with polarity-regulating kinase PAR-1b. (2010)
  • Author(s): Yamashita K, Suzuki A, Satoh Y, Ide M, Amano Y, Masuda-Hirata M, Hayashi Y.K, Hamada K, Ogata K, Ohno S
  • Journal Title: Biochem Biophys Res Commun. Volume: 391(1) Pages: 812-817
  • Peer Reviewed
• [Presentation] Signaling Crosstalk between Cell Polarity and Cell Growth/Apoptosis (2012)
  • Author(s): Ohno S
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県).
  • Year and Date: 2012-12-07
• [Presentation] Epithelial cell polarity and the PAR-aPKC complex (2012)
  • Author(s): Ohno S
  • Organizer: Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
  • Place of Presentation: Merida, Mexico.
  • Year and Date: 2012-11-02
• [Presentation] Integrated regulation of PIKK-mediated stress responses by AAA+ proteins RUVBL1 and RUVBL2 (2012)
  • Author(s): Ohno S
  • Organizer: First International Workshop on Pontin (RUVBL1) and Reptin (RUVBL2)
  • Place of Presentation: Bordeaux, France
  • Year and Date: 2012-10-18
• [Presentation] The regulators and the effectors of the aPKC-PAR. (2012)
  • Author(s): Ohno S
  • Organizer: The regulators and the effectors of the aPKC-PAR. SFB Symposium 2012 "Molecular Cell Dynamics"
  • Place of Presentation: Munster, Germany.
  • Year and Date: 2012-06-01
• [Presentation] 細胞極性化の原理と生理機能 (2012)
  • Author(s): 大野茂男
  • Organizer: 第117回解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県).
  • Year and Date: 2012-03-28
• [Presentation] 細胞極性制御因子aPKCによる乳腺上皮前駆細胞の増殖制御と乳がんにおけるその破綻. (2012)
  • Author(s): 秋本和憲, 長嶋洋治, 石黒 斉, 鄭 允文, 佐々木和教, 谷口英樹, 野田哲生, 大野茂男
  • Organizer: 第３０回日本ヒト細胞学会学術集会シンポジウム
  • Place of Presentation: 梅田スカイビル(大阪府）
• [Presentation] Specific inhibition of SMG-8 rescues effectively the mutant phenotypes exacerbated by nonsense-mediated mRNA decay without cell toxicity. (2012)
  • Author(s): Yamashita A, Usuki F, Ohno S
  • Organizer: 2012 CSHL Meeting on Translational Control
  • Place of Presentation: Cold Spring Harbor Laboratory（NY, USA）
• [Presentation] A Novel Microtubule Binding Protein, MARKAP1, Regulates Stability of the Golgi-Nucleated Microtubules and Directional Cell Migration. (2012)
  • Author(s): Satoh Y, Hayashi K, Amano Y, Takahashi M, Hayashi I, Ohno S, Suzuki A
  • Organizer: American Society for Cell Biology ( ASCB) 2012 Annual Meeting
  • Place of Presentation: Moscone Center, San Francisco（San Francisco, USA）
• [Presentation] Cell polarity and cancer. 2011 International Symposium on Infectious Disease and Signal Transduction (2011)
  • Author(s): Ohno S
  • Organizer: 2011 Taiwan-Japan Joint Symposium on Cell Signaling and Gene Regulation
  • Place of Presentation: Tainan City, Taiwan
  • Year and Date: 2011-11-20
• [Presentation] Epithelial cell polarity and the Par-aPKC complex (2011)
  • Author(s): Ohno S
  • Organizer: 5th International Epithelial-Mesenchymal Transition Meeting
  • Place of Presentation: Biopolis, Singapore
  • Year and Date: 2011-10-13
• [Presentation] Cell polarity and the maintenance and disruption of epithelial tissues (2011)
  • Author(s): Ohno S
  • Organizer: US-Japan Summer Conference on Cancer
  • Place of Presentation: Duarte, CA, USA.
  • Year and Date: 2011-08-05
• [Presentation] ASPP2 regulates epithelial cell polarity through the PAR complex. (2010)
  • Author(s): Ohno S
  • Organizer: The 50th American Society for Cell Biology Annual Meeting
  • Place of Presentation: Philadelphia, USA(招待講演)
  • Year and Date: 2010-12-15
• [Presentation] KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity in epithelial cells. (2010)
  • Author(s): Yoshihama Y, Sasaki K, Horokoshi Y, Ohtsuka T, Hakuno F, Takahashi S-I, Suzuki A, Ohno S, Chida K
  • Organizer: The 50th American Society for Cell Biology Annual Meeting
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2010-12-14
• [Presentation] 微量RNAからの次世代シークエンスライブラリー構築法の改善 (2010)
  • Author(s): 馬場しほ, 山下暁朗, 大野茂男
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] 細胞極性制御因子aPKCλによる上皮前駆細胞の増殖制御とその破綻 (2010)
  • Author(s): 秋本和憲, 鄭允文, 長嶋洋治, 石黒斉, 佐々木和教, 金民大, 李斌, 角和卓, 谷口英樹, 野田哲生, 大野茂男
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-10
• [Presentation] Axon formation in neocortical neurons depends on the stage-specific stabilization of microtubules by DLK-JNK pathway. (2010)
  • Author(s): Hirai S-I, Banba Y, Satake T, Ohno S
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Regulatory mechanism of kinesin-1 motor activity through JIP1. (2010)
  • Author(s): Satake T, Hirano H, Yamanaka Y, Ohno S, Hirai S-I
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-08
• [Presentation] Maintenance of dendritic spine morphology by PAR1b through regulation of microtubule dynamics. (2010)
  • Author(s): 林健二, 鈴木厚, 大野茂男
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] KIBRA suppresses apical exocytosis through inhibition of aPKC kinase activity in epithelial cells. (2010)
  • Author(s): Yoshihama Y, Sasaki K, Horokoshi Y, Ohtsuka T, Hakuno F, Takahashi S-I, Suzuki A, Ohno S, Chida K
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] 細胞極性によるHippo経路の抑制がマウス着床前胚における位置依存的な細胞分化をつかさどる (2010)
  • Author(s): 平手良和, 平原志乃, 井上健一, 中尾和貴, 鈴木厚, 秋本和憲, 大野茂男, 平井孝明, 千田和広, Marikawa Y, Vernadeth A, 佐々木洋
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(ポスター発表)
  • Year and Date: 2010-12-07
• [Presentation] 細胞極性によるHippo経路の抑制がマウス着床前胚における位置依存的な細胞分化をつかさどる (2010)
  • Author(s): 平手良和, 平原志乃, 井上健一, 中尾和貴, 鈴木厚, 秋本和憲, 大野茂男, 平井孝明, 千田和広, Marikawa Y, Vernadeth A, 佐々木洋
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(口頭発表)
  • Year and Date: 2010-12-07
• [Presentation] mRNA品質管理機構制御因子SMG-1の構想と生理機能 (2010)
  • Author(s): 山下暁朗, Ernesto A P, Israel F S, 番場由美, 星野耕二, 上村博司, Oscar L I, 大野茂男
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Signaling Crosstalk between Cell Polarity and Cell Growth/Apoptosis. (2010)
  • Author(s): Ohno S
  • Organizer: 第33回日本分子生物学会年会第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド(招待講演)
  • Year and Date: 2010-12-07
• [Presentation] The cell polarity proteins, aPKC-Par complex, regulate the turnover of nephrin to maintain slit diaphragm integrity. (2010)
  • Author(s): Satoh D, Harita Y, Daimon C, Hirose T, Ohno S
  • Organizer: AMERICAN SOCIETY OF NEPHROLOGY Renal Week 2010
  • Place of Presentation: Colorado, USA
  • Year and Date: 2010-11-19
• [Presentation] aPKC and IL-6 expression in prostate cancer tissues. (2010)
  • Author(s): Ishiguro H, Akimoto K, Nagashima Y, Kagawa E, Sasaki T, Sano J, Takagawa R, Sasaki K, Fujinami K, Aoki I, Ohno S, Kubota Y, Uemura H
  • Organizer: The 69th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Osaka International Convention Center
  • Year and Date: 2010-09-24
• [Presentation] Regulation of Girdin/GIV/Ccdc88A expression by PAR-3 is required for establishment of epithelial cell polarity. (2010)
  • Author(s): Sasaki K, Ohno S
  • Organizer: The 69th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: Osaka International Convention Center
  • Year and Date: 2010-09-22
• [Presentation] Inside-out signalling effects on extracellular membrane and polarity (2010)
  • Author(s): Ohno S
  • Organizer: The 8th International Podocyte Conference
  • Place of Presentation: Bristol, UK
  • Year and Date: 2010-06-08
• [Presentation] Inside-out signalling effects on extracellular membrane and polarity. (2010)
  • Author(s): Ohno S
  • Organizer: The 8th International Podocyte Conference
  • Place of Presentation: Bristol UK(招待講演)
  • Year and Date: 2010-06-08
• [Presentation] 新規PAR1結合タンパク質p250は, 微小管安定化タンパク質である (2010)
  • Author(s): 鈴木厚, 佐藤由典, 秋津真志, 天野芳子, 大野茂男
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-20
• [Presentation] Apoptosis-Stimulating Protein of p53 (ASPP2) isinvolved in the PAR Complex to Regulate Epithelial Cell Polarity. (2010)
  • Author(s): 叢偉立, 廣瀬智威, 山下暁朗, 水野恵子, 張田豊, 平野 久, 大野茂男
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-19
• [Presentation] A Novel Microtubule Binding Protein, MARKAP, plays essential roles for the Golgi-Ribbon Formation by Regulating Golgi-Nucleated Microtubules.
  • Author(s): Satoh Y, Hayashi K, Amano Y, Maki T, Hayashi I, Ohno S, Suzuki A
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe International Conference Center.The Kobe Chamber of Commerce and Industry(兵庫県）
• [Presentation] MARKAP1 cooperates with PAR-1/MARK for development of epithelial polarity by regulating microtubule organization.
  • Author(s): Akitsu M, Satoh Y, Amano Y, Ohno S, Suzuki A
  • Organizer: Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology
  • Place of Presentation: Kobe International Conference Center.The Kobe Chamber of Commerce and Industry(兵庫県）
• [Presentation] The regulators and the effectors of the aPKC-PAR.
  • Author(s): Ohno S
  • Organizer: SFB Symposium 2012 "Molecular Cell Dynamics"
  • Place of Presentation: Max Planck Institut（ミュンスター, Germany)
  • Invited
• [Presentation] The nuclear localization and overexpression of atypical protein kinase C in cervical cancer correlate with prognosis.
  • Author(s): Mizushima T, Akimoto K, Nagashima Y, Mogami T, Sato M, Miyagi E, Kameda Y, Inayama Y, Ohashi K, Aoki I, Ohno S, Hirahara F
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌、さっぽろ芸術館、札幌市教育文化会館（北海道）
• [Presentation] Integrated regulation of PIKK-mediated stress responses by AAA+ proteins RUVBL1 and RUVBL2.
  • Author(s): Ohno S
  • Organizer: First International Workshop on Pontin (RUVBL1) and Reptin (RUVBL2)
  • Place of Presentation: The European Institute for Chemistry and Biology (IECB)（Bordeaux, France）
  • Invited
• [Presentation] Epithelial cell polarity and the PAR-aPKC complex.
  • Author(s): Ohno S
  • Organizer: Molecular Structure and Function of the Apical Junctional Complex in Epithelia and Endothelia
  • Place of Presentation: Hyatt Regency（Merida, Mexico）
  • Invited
• [Presentation] JNK Facilitates the Formation of Epithelial Cell Sheet.
  • Author(s): Furukawa K, Ohno S, Hirai S-I
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] Analysis of Physiological Function of MARKAP1 in the Cerebellum.
  • Author(s): Satake T, Hayashi K, Yamashita K, Satoh Y, Ohno S, Suzuki A
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] Transcriptional Timing Determines the Stability of Interferon-beta mRNA.
  • Author(s): Abe K, Ishigami T, Ann-Bin S, Ohno S, Umemura S, Yamashita A
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] SMG-1, PIKK Family Kinase, Regulates mRNA Surveillance.
  • Author(s): Yamashita A, Kurosawa H, Ohno S
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] p50 Regulates Epithelial Cell Polarity by Inhibiting E-cadherin endocytosis.
  • Author(s): Fujikawa Y, Hirose T, Harita Y, Sasaki K, Yamashita K, Yamashita A, Hirano H, Ohno S
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡（福岡県）
• [Presentation] A novel microtubule binding protein, MARKAP1, cooperates with PAR-1 for the development of the lateral microtubule bundles in epithelial cells.
  • Author(s): Akitsu M, Satoh Y, Amano Y, Yamashita K, Yamashita A, Maki T, Hayashi I, Hirano H, Ohno S, Suzuki A
  • Organizer: American Society for Cell Biology ( ASCB) 2012 Annual Meeting
  • Place of Presentation: Moscone Center, San Francisco（San Francisco, USA）
• [Presentation] ガン抑制遺伝子Lglは、VprBPと結合することによって、単層の上皮構造を維持している.
  • Author(s): 山下 和成, 井出 真理子, 村松 玲子, 鈴木 厚, 平野 久, 大野 茂男
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学 クラーク会館、学術交流会館(北海道)
• [Presentation] Par-1結合タンパク質p250は、微小管の結束化および安定化に関与するタンパク質である.
  • Author(s): 佐藤 由典, 秋津 真志, 林 健二, 天野 芳子, 大野 茂男, 鈴木 厚
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学 クラーク会館、学術交流会館(北海道)
• [Presentation] Maintenance of Dendritic Spine Morphology by PAR1b through Regulation of Microtubule Dynamics.
  • Author(s): 林 健二, 鈴木 厚, 平井 秀一, 栗原 靖之, Casper C Hoogenraad, 大野 茂男
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学 クラーク会館、学術交流会館(北海道)
• [Presentation] aPKClambda regulates mammary luminal progenitor cell propagation and its disruption in a subpopulation of breast cancer.
  • Author(s): Akimoto K, Nagashima Y, Ishiguro H, Zheng Y W, Osato N, Sasaki K, Kojima Y, Tamura T, Taniguchi H, Noda T, Ohno S
  • Organizer: US-Japan Summer Conference on Cancer, Diabetes and Inflammation
  • Place of Presentation: Beckman Center Lecture Hall（Duarte, CA, USA）
• [Presentation] PAR-3 mediates the expression of Girdin/GIV/Ccdc88a to regulate epithelial cell polarity.
  • Author(s): Sasaki K, Kakuwa T, Koga H, Ohno S
  • Organizer: US-Japan Conference on Inflammation, Diabetes and Cancer
  • Place of Presentation: Beckman Center Lecture Hall（Duarte, CA, USA）
• [Presentation] Cell polarity and the maintenance and disruption of epithelial tissues.
  • Author(s): Ohno S
  • Organizer: US-Japan Summer Conference on Cancer, Diabetes and Inflammation
  • Place of Presentation: Beckman Center Lecture Hall（Duarte, CA, USA）
  • Invited
• [Presentation] 限外濾過膜としてのスリット膜のターンオーバーとそのメカニズム.
  • Author(s): 佐藤大輔, 張田 豊, 大門主税, 栗原秀剛, 廣瀬智威, 大野茂男
  • Organizer: 第100回東京腎生理集談会
  • Place of Presentation: 第100回東京腎生理集談会（東京都）
• [Presentation] aPKC λ regulates mammary luminal progenitor cell propagation and it’s disruption in a subpopulation of breast cancer.
  • Author(s): Akimoto K, Nagashima Y, Ishiguro H, Zheng Y-W, Sasaki K, Kojima Y, Taniguchi H, Ohno S
  • Organizer: 70th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] Prognostic impact of aPKC λ/ι expression in pancreatic cancer.
  • Author(s): Kato S, Akimoto K, Nagashima Y, Ishiguro H, Kobayashi N, Maeda S, Ohno S, Nakajima A
  • Organizer: 70th Annual Meeting of the Japanese Cancer Association
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] Epithelial cell polarity and the Par-aPKC complex.
  • Author(s): Ohno S
  • Organizer: 5th International Epithelial-Mesenchymal Transition Meeting
  • Place of Presentation: College of Medicine, National Cheng Kung University.Taipei Medical University（Biopolis, Singapore）
  • Invited
• [Presentation] Cell polarity and cancer.
  • Author(s): Ohno S
  • Organizer: 2011 International Symposium on Infectious Disease and Signal Transduction, 2011 Taiwan-Japan Joint Symposium on Cell Signaling and Gene Regulation
  • Place of Presentation: College of Medicine, National Cheng Kung University（Tainan City, Taiwan）
  • Invited
• [Presentation] Maintenance of Dendritic Spine Morphology by PAR1b through Regulation of Microtubule Growth.
  • Author(s): Hayashi K, Suzuki A, Hoogenraad C. C, Ohno S
  • Organizer: 2011 The American Society for Cell Biology Annual Meeting
  • Place of Presentation: Colorado Convention Center（Denver, Colorado, USA）
• [Presentation] PAR3 is required for restricting symmetric proliferative divisions in mouse neutral precursor cells.
  • Author(s): Hirose T, Sugitani Y, Kusaka C, Noda T, Ohno S
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Molecular mechanisms regulating the turnover of nephrin; the role of cell polarity regulator, aPKC.
  • Author(s): Satoh D, Harita Y, Daimon C, Hirose T, Kurihara H, Ohno S
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] IL-6 遺伝子発現における mRNA 転写後制御機構の解析.
  • Author(s): 安部 開人, 山下 暁朗, Ann-Bin Shyu, 石上 友章, 大野 茂男, 梅村 敏
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] A new microtubule binding protein, p250, regulates the directional migration of epithelial cells.
  • Author(s): 佐藤 由典, 秋津 真志, 林 健二, 天野 芳子, 林 郁子, 大野 茂男, 鈴木 厚
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] 細胞極性化の原理と生理機能.
  • Author(s): 大野茂男
  • Organizer: 第117回解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス（山梨県）
  • Invited
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/Japanease/indexJ.html
• [Remarks] 大野研究室 分子細胞生物学 Cell Signaling
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/Japanease/indexJ.html
• [Remarks] 大野研究室 分子細胞生物学 Cell Signaling
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos/Japanease/indexJ.html
• [Remarks]
  • URL: http://www-user.yokohama-cu.ac.jp/~ohnos//Japanease/indexj.html