Structure, formation, and contraction of the contractile ring
Project/Area Number |
22247031
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Cell biology
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Research Institution | Gakushuin University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
USUKURA Jiro 名古屋大学, エコトピア科学研究所, 教授 (30143415)
KOJIMA Shin-ichiro 学習院大学, 理学部, 助教 (30519210)
KASHIWAZAKI Jun 学習院大学, 理学部, 助教 (70570654)
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Research Collaborator |
KASHIWAZAKI Jun
TAKAGI Tomoko
BALASUBRAMANIAN Mohan K.
INOUE Shinya
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Project Period (FY) |
2010-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥39,910,000 (Direct Cost: ¥30,700,000、Indirect Cost: ¥9,210,000)
Fiscal Year 2013: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2011: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2010: ¥14,170,000 (Direct Cost: ¥10,900,000、Indirect Cost: ¥3,270,000)
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Keywords | 細胞分裂 / 細胞質分裂 / 収縮環 / アクチン / ミオシン / 細胞 / タンパク質 / 微細構造 / 生体分子 / シグナル伝達 / 電子顕微鏡 / 細胞周期 / 細胞運動 |
Outline of Final Research Achievements |
In cell division, cytokinesis takes place by contraction of the contractile ring at the mid region of the cell. I isolated the cell ghost from dividing fission east cells, in which the contractile ring was attached to the cell membrane, and demonstrated for the first time the contraction of the contractile ring by addition of ATP. It was shown using this system that actin depolymerization was not necessary in the contraction. It was also suggested that myosin V is involved in movement of actin to the division site during contractile ring formation. The actin polymerizing protein uDia was shown to co-localize with actin in the contractile ring of dividing sea urchin eggs. Furthermore, it was shown that actin flows in the frog egg extract encapsulated in artificial lipid vesicles.
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Report
(5 results)
Research Products
(38 results)
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[Journal Article] In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics.2013
Author(s)
Mishra, M., Kashiwazaki, J., Takagi, T., Srinivasan, R., Huang, Y., Balasubramanian, M. K., and Mabuchi, I.
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Journal Title
Nature Cell Biology
Volume: 15
Issue: 7
Pages: 853-859
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics.2014
Author(s)
Mishra, M., Kashiwazaki, J., Takagi, T., Srinivasan, R., Huang, Y., Balasubramanian, M. K., and Mabuchi, I.
Organizer
58th Annual Meeting of the Biophysical Society
Place of Presentation
サンフランシスコ
Year and Date
2014-02-15 – 2014-02-19
Related Report
Invited
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[Presentation] 収縮環のin vitro実験系の開発による収縮機構の研究2013
Author(s)
馬渕一誠、柏崎隼、Mishra,M., 高木智子、Srinivasan, R., Huang, Y., and Balasubramanian, M.
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸
Year and Date
2013-12-03 – 2013-12-06
Related Report
Invited
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[Presentation] Cytokinesis in vitro and in vivo.2013
Author(s)
Balasubramanian, M., Mishra, M., Kashiwazaki, J., Tao, E. Y., Huang, Y., Huang, J. J., Takagi, T., Subramanian, D., Xie, T., Padmanabhan, A., Haochen, J., Wedlich-Soldner, R. and Mabuchi, I.
Organizer
Cell Biology of Yeasts
Place of Presentation
Cold Spring Harbor, NY
Year and Date
2013-11-05 – 2013-11-09
Related Report
Invited
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[Presentation] In vitro contraction of cytokinetic ring depends on myosin II but not on actin dynamics.2013
Author(s)
Mishra, M., Kashiwazaki, J., Takagi, T., Srinivasan, R., Huang, Y., Balasubramanian, M., and Mabuchi, I.
Organizer
EMBO Conference on Fission Yeast: Pombe 2013
Place of Presentation
ロンドン
Year and Date
2013-06-24 – 2013-06-29
Related Report
Invited
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[Presentation] Cytokinesis in vitro and in vivo.2013
Author(s)
Balasubramanian, M., Tao, E. Y., Mishra, M., Kashiwazaki, J., Huang, Y., Huang, J. J., Takagi, T., Subramanian, D., Xie, T., and Mabuchi, I.
Organizer
EMBO Conference in Fission Yeast
Place of Presentation
ロンドン
Year and Date
2013-06-24 – 2013-06-29
Related Report
Invited
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[Book] 生物学事典2010
Author(s)
馬渕一誠(執筆分担)
Total Pages
1615
Publisher
東京化学同人
Related Report
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