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Construction of a protozoan infection therapeutic vaccine development platform technology using antigen-displaying baculovirus

Research Project

Project/Area Number 22248009
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionShizuoka University

Principal Investigator

PARK Enoch Y.  静岡大学, グリーン科学技術研究所, 教授 (90238246)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥46,150,000 (Direct Cost: ¥35,500,000、Indirect Cost: ¥10,650,000)
Fiscal Year 2013: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2011: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2010: ¥16,510,000 (Direct Cost: ¥12,700,000、Indirect Cost: ¥3,810,000)
Keywordsカイコ / ワクチン / 抗原提示 / バキュロウイルス / バイオテクノロジー / 蚕
Research Abstract

In this study, we build the foundation of component vaccine development of neosporosis. Five candidate antigens were successfully expressed and purified in silkworm. Bombyx mori nucleopolyhedrovirus (BmNPV) particles displaying Neospora caninum antigens (NcSAG1, NcSRS2 and NcMIC3) purified from silkworm larvae were constructed to vaccinate mice against N. caninum. Antigen-specific IgG2a was predominantly produced in mice by immunization with NcSAG1-displaying BmNPV particles compared to IgG1, and induction of IFN-gamma was dominant, indicating that antigen-displaying BmNPV particles can elicit a Th1 immune response in mice. Semi-quantitative PCR analysis revealed that immunization with each antigen-displaying BmNPV particle purified from silkworms partially protected mice from cerebral N. caninum infection. These results suggest that antigen-displaying BmNPV particles purified from silkworms can provide an alternative method of a new generation of N. caninum vaccines.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (49 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (23 results) Remarks (6 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Tracking Neospora caninum parasites using chimera monoclonal antibodies against its surface antigen-related sequences (rNcSRS2)2014

    • Author(s)
      Jinhua Dong, Takahiro Otsuki, Tatsuya Kato, and Enoch Y.Park
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 177巻 Issue: 3 Pages: 351-357

    • DOI

      10.1016/j.jbiosc.2013.09.003

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Display of Neospora caninum surface protein related sequence 2 on Rous sarcoma virus-derived gag protein virus-like particles2013

    • Author(s)
      Vipin Kumar Deo, Katsuhiko Yoshimatsu, Takahiro Otsuki, Jinhua Dong, Tatsuya Kato, Enoch Y.Park
    • Journal Title

      J. Biotechnol.

      Volume: 165巻 Issue: 1 Pages: 69-75

    • DOI

      10.1016/j.jbiotec.2013.02.013

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of anti-Neospora antibodies in bovine serum by using spiky Au-CdTe nanocomplexes2013

    • Author(s)
      HongJin Zhou, Jinhua Dong, Vipin Kumar Deo, Enoch Y.Park, Jaebeom Lee
    • Journal Title

      Sensor Actuat B

      Volume: 178巻 Pages: 192-199

    • DOI

      10.1016/j.snb.2012.12.078

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus2013

    • Author(s)
      Jinhua Dong, Akira Sakurai, Namiko Nomura, Enoch Y.Park, Futoshi Shibasaki, Hiroshi Ueda
    • Journal Title

      PLoS One

      Volume: 8巻 Issue: 4 Pages: e61158-e61158

    • DOI

      10.1371/journal.pone.0061158

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression, purification and antigenicity of Neospora caninum-antigens using silkworm larvae targeting for subunit vaccines2013

    • Author(s)
      Takahiro Otsuki, Jinhua Dong, Tatsuya Kato and Enoch Y.Park
    • Journal Title

      Vet. Parasitol.

      Volume: 192巻 Issue: 1-3 Pages: 284-287

    • DOI

      10.1016/j.vetpar.2012.09.038

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Construction of new ligation-independent cloning vectors for the expression and purification of recombinant proteins in silkworms using BmNPV bacmid system2013

    • Author(s)
      Tatsuya Kato, James R. Thompson, Enoch Y. Park
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 5 Pages: e64007-e64007

    • DOI

      10.1371/journal.pone.0064007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of two murine antibodies against Neospora caninum using phage display technology and application on the detection of N. canimum2013

    • Author(s)
      Jinhua Dong, Takahiro Otsuki, Tatsuya Kato, Tetsuya Kohsaka, Kazunori Ike, and Enoch Y. Park
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 1 Pages: e53264-e53264

    • DOI

      10.1371/journal.pone.0053264

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression, purification and antigenicity of Neospora caninum-antigens using silkworm larvae targeting for subunit vaccines2013

    • Author(s)
      Takahiro Otsuki, Jinhua Dong, Tatsuya Kato and Enoch Y. Park
    • Journal Title

      Vet. Parasitol.

      Volume: 192 Issue: 2 Pages: 284-287

    • DOI

      10.1007/s11814-013-0003-1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improvement of the transcriptional strength of baculovirus very late polyhedrin promoter by repeating its untranslated leader sequences and co-expression with the primary transactivator2012

    • Author(s)
      Tatsuya Kato, Suganthi Lavender Manohar, Shin Kanamasa, Makoto Ogata, and Enoch Y.Park
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 113巻 Issue: 6 Pages: 694-696

    • DOI

      10.1016/j.jbiosc.2012.01.010

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins2012

    • Author(s)
      Jinhua Dong, Takahiro Otsuki, Tatsuya Kato, and Enoch Y.Park
    • Journal Title

      BMC Biotechnol.

      Volume: 12巻 Issue: 1 Pages: 19-19

    • DOI

      10.1186/1472-6750-12-19

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of human papillomavirus 6b L1 protein in silkworm larvae and enhanced green fluorescent protein displaying on its virus-like particles2012

    • Author(s)
      Muthukutty Palaniyandi, Tatsuya Kato and Enoch Y. Park
    • Journal Title

      SpringerPlus

      Volume: 1 Issue: 1

    • DOI

      10.1186/2193-1801-1-29

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding2011

    • Author(s)
      Tatsuya Kato, Fumiaki Suzuki, and Enoch Y.Park
    • Journal Title

      BMC Biotechnology

      Volume: 11巻 Issue: 1 Pages: 60-60

    • DOI

      10.1186/1472-6750-11-60

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of RSV-gag virus like particle in insect cell lines and silkworm larvae2011

    • Author(s)
      Vipin Kumar Deo, Yoshitaka Tsuji, Tomomi Yasuda, Tatsuya Kato, Naonori Sakamoto, Hisao Suzuki, Enoch Y.Park
    • Journal Title

      J. Viol. Methods

      Volume: 177巻 Issue: 2 Pages: 147-152

    • DOI

      10.1016/j.jviromet.2011.07.012

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of Rous sarcoma virus-like particles displaying human transmembrane protein in silkworm larvae and its application to ligand-receptor binding assay2011

    • Author(s)
      Yoshitaka Tsuji, Vipin Kumar Deo, Tatsuya Kato, and Enoch Y Park
    • Journal Title

      J. Biotechnol.

      Volume: 155巻 Issue: 2 Pages: 185-192

    • DOI

      10.1016/j.jbiotec.2011.07.008

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced Gene Expression in Insect Cells and Silkworm Larva by Modified Polyhedrin Promoter using Repeated Burst Sequence and Very Late Transcriptional Factor-12010

    • Author(s)
      Lavender Suganthy Manohar, Shin Kanamasa, Takuya Nishida, Tatsuya Kato, Enoch Y.Park
    • Journal Title

      Biotechnol.Bioeng.

      Volume: 107 Pages: 909-916

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] New strategy for rapid isolation of stable cell lines from DNA-transformed insect cells using fluorescence activated cell-sorting2010

    • Author(s)
      Tatsuya Kato, Kengo Yoshizuka, Enoch Y.Park
    • Journal Title

      J.Biotechnol.

      Volume: 147 Pages: 102-107

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Silkworm expression system as a platform technology in life science2010

    • Author(s)
      Tatsuya Kato, Mizuho Kajikawa, Katsumi Maenaka, Enoch Y.Park
    • Journal Title

      Appl.Microbiol.Biotechnol.

      Volume: 85 Pages: 459-470

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Neospora caninum-antigens and antigen displaying baculovirus using silkworm larvae targeting for Neospora vaccine2013

    • Author(s)
      Enoch Y.Park, Takahiro Otsuki, Jinhua Dong, Tatsuya Kato
    • Organizer
      2013 KSBB Fall meeting and International Symposium
    • Place of Presentation
      BEXCO(韓国釜山)
    • Year and Date
      2013-10-18
    • Related Report
      2013 Final Research Report
  • [Presentation] Neospora caninum 抗原タンパク質を提示したバキュロウイルスの作製とマウスの免疫化2013

    • Author(s)
      大月 隆寛、董 金華、加藤 竜也、高坂 哲也、朴 龍洙
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内キャンパス(仙台)
    • Year and Date
      2013-03-25
    • Related Report
      2013 Final Research Report
  • [Presentation] Silkworm larvae as a potential protein factory-Silkworm expression system using bacmid2013

    • Author(s)
      Enoch Y.Park
    • Organizer
      CELL Tech 2013
    • Place of Presentation
      Crowne Plaza (San Diego、USA)
    • Year and Date
      2013-01-22
    • Related Report
      2013 Final Research Report
  • [Presentation] ネオスポラ症及び抗ネオスポラ原虫の検出法の開発2013

    • Author(s)
      朴 龍洙、董 金華、 大月 隆寛、加藤 竜也
    • Organizer
      第7回バイオ関連化学シンポジウム
    • Place of Presentation
      名古屋市名古屋大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Neospora caninum-antigens and antigen displaying baculovirus using silkworm larvae targeting for Neospora vaccine2013

    • Author(s)
      Enoch Y. Park, Takahiro Otsuki, Jinhua Dong, Tatsuya Kato
    • Organizer
      2013 KSBB Fall meeting and International Symposium
    • Place of Presentation
      BEXCO in Busan Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] High- or medium-throughput protein expression and purification in silkworm pupae2013

    • Author(s)
      Tatsuya Kato, James R. Thompson, Enoch Y. Park
    • Organizer
      Shizuoka University International Symposium 2013 - Directions of Interdisciplinary Domain Research in Japan-Europe Partnership -
    • Place of Presentation
      Granship in Shizuoka
    • Related Report
      2013 Annual Research Report
  • [Presentation] カイコ発現系を用いたネオスポラカニナム抗原タンパク質の発現、精製及びそれらを用いたマウスの免疫2013

    • Author(s)
      Mai Yoshimoto, Jinha Dong, Tatsuya Kato,Tetsuya Kousaka, Kazunori Ike, Yumino Matsumoto, Kazunori Ike, and Enoch Y. Park
    • Organizer
      シーズ&ニーズビジネスマッチング研究発表会ー高齢化社会・健康・食品産業ー
    • Place of Presentation
      静岡市ホテルアソシア静岡3F駿河II
    • Related Report
      2013 Annual Research Report
  • [Presentation] Neospora caninum抗原タンパク質を提示したバキュロウイルスの作製とマウスの免疫化2013

    • Author(s)
      大月 隆寛、董 金華、加藤 竜也、高坂 哲也、朴 龍洙
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内北キャンパス(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Display of Neospora caninum Surface Protein Related Sequence 2 (NcSRS2) on Rous sarcoma Virus Derived gag Protein Virus-like Particles (VLPs)2013

    • Author(s)
      Vipin Kumar Deo, Katsuhiko Yoshimatsu, Takahiro Otsuki, Jinhua Dong, Tatsuya Kato and Enoch Y Park
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学川内北キャンパス(仙台)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Silkworm larvae as a potential protein factory-Silkworm expression system using bacmid2013

    • Author(s)
      Enoch Y. Park
    • Organizer
      Cell Tech 2013
    • Place of Presentation
      Crowne Plaza San Diego (USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 量子ドット/金ナノ粒子複合体を用いた牛ネオスポラ症の検出2012

    • Author(s)
      董金華、周 宏建、李 可範、朴 龍洙
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Year and Date
      2012-10-25
    • Related Report
      2013 Final Research Report
  • [Presentation] 量子ドット/金ナノ粒子複合体を用いた牛ネオスポラ症の検出2012

    • Author(s)
      董金華、周 宏建、李 可範、朴 龍洙
    • Organizer
      第64回日本生物工学会大会
    • Place of Presentation
      神戸国際会議場(神戸)
    • Related Report
      2012 Annual Research Report
  • [Presentation] A new diagnostic method for neosporosis in cattle using recombinant Neospora caninum proteins2012

    • Author(s)
      Jinhua Dong, Takahiro Otsuki, Tatsuya Kato, and Enoch Y. Park
    • Organizer
      15th International Biotechnology Symposium and Exhibition
    • Place of Presentation
      EXCO, Daegu (Korea)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Display of Neospora caninum antigenic proteins (SRS2, SAG1, MIC3) onto the baculovirus surface2012

    • Author(s)
      Takahiro Otsuki, Jinhua Dong, Tatsuya Kato, Enoch Y Park
    • Organizer
      15th International Biotechnology Symposium and Exhibition
    • Place of Presentation
      EXCO, Daegu (Korea)
    • Related Report
      2012 Annual Research Report
  • [Presentation] BmNPVバクミド‐カイコ発現系を用いたNeospora caninum 抗原タンパク質の発現2011

    • Author(s)
      大月 隆寛,加藤 竜也,朴 龍洙
    • Organizer
      第63回日本生物工学会大会
    • Place of Presentation
      東京農工大学(東京)
    • Year and Date
      2011-09-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Synthesis of chelate molecular-based tetravalent glycoside influenza virus inhibitor, Proceeding p37(2010)2011

    • Author(s)
      Seiichiro Umemura, Makoto Ogata, Chika Hashimoto, Enoch Y.Park, Taichi Usui
    • Organizer
      Second Joint Symposium TU Braunschweig-Shizuoka University
    • Place of Presentation
      TU Braunschweig in Germany
    • Year and Date
      2011-08-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression of Rous Sarcoma Virus gag protein forming virus like particles in insect cells, Proceeding p28, 2010.2011

    • Author(s)
      Vipin K Deo, Tomomi Yasuda, Tsuji Yoshitaka, Enoch Y.Park
    • Organizer
      Second Joint Symposium TU Braunschweig-Shizuoka University
    • Place of Presentation
      TU Braunschweig in Germany
    • Year and Date
      2011-08-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Display of human prorenin receptor on baculovirus using silkworm larvae and its application on detection of protein-protein interaction, ICCMB proceeding page75, 2010.2011

    • Author(s)
      Tatsuya Kato, Enoch Y Park
    • Organizer
      The 2nd International Conference of Cellular & Molecular Bioengineering
    • Place of Presentation
      Nangyang Technical University in Singapore
    • Year and Date
      2011-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression of Neospora caninum antigenic proteins using Bombyx morinucleopolyhedrovirus(BmNPV)bacmid expression system, Proceeding p71-72, 20112011

    • Author(s)
      Takahiro Otsuki, Tatsuya Kato, Enoch Y.Park
    • Organizer
      1st Zhejiang Univ.-Shizuoka Univ.Student Workshop
    • Place of Presentation
      Zhejiang Univ.in China
    • Year and Date
      2011-03-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] VLPs display technology a step towards efficient vaccination platform using stable insect cell line and silkworm 1st Zhejiang Univ.-Shizuoka Univ.Student Workshop. Proceeding p22.24, 20112011

    • Author(s)
      Vipin Kumar Deo, Yoshitaka Tsuji, Tomomi Yasuda, Tatsuya Kato, Naonori Sakamoto, Hisao Suzuki, Enoch Y.Park
    • Organizer
      1st Zhejiang Univ.-Shizuoka Univ.Student Workshop
    • Place of Presentation
      Zhejiang Univ.in China
    • Year and Date
      2011-03-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Expression of Neospora caninum proteons and their application in diagnostic of neosporosis in cattle

    • Author(s)
      Jinhua Dong, Takahiro Otsuki, Tatsuya Kato, and Enoch Y. Park
    • Organizer
      International Symposium on Global Young Fellowship for Functional Development of Life Science
    • Place of Presentation
      KyungPook National University (Korea)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Display of Neospora caninum antigenic proteins (SRS2, SAG1, MIC3) onto the baculovirus surface

    • Author(s)
      Takahiro Otsuki, Jinhua Dong, Tatsuya Kato, Enoch Y Park
    • Organizer
      International Symposium on Global Young Fellowship for Functional Development of Life Science
    • Place of Presentation
      KyungPook National University (Korea)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Detection of anti-neospora antibodies on bovine serum by Au-CdTe nanocomplex

    • Author(s)
      Hongjian Zhou, Jinhua Dong, Enoch Y. Park, Jaebeom Lee
    • Organizer
      International Symposium on Global Young Fellowship for Functional Development of Life Science
    • Place of Presentation
      KyungPook National University (Korea)
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.shizuoka.ac.jp/c/biotech/

    • Related Report
      2013 Final Research Report
  • [Remarks] 静岡大学生物工学研究室

    • URL

      http://www.agr.shizuoka.ac.jp/c/biotech/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 静岡大学グリーン科学技術研究所

    • URL

      http://www.green.shizuoka.ac.jp

    • Related Report
      2013 Annual Research Report
  • [Remarks] 生物工学研究室

    • URL

      http://www.agr.shizuoka.ac.jp/c/biotech/

    • Related Report
      2012 Annual Research Report
  • [Remarks] 静岡大学ナノバイオ科学推進室

    • URL

      http://gsst.shizuoka.ac.jp/nano-bio/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.agr.shizuoka.ac.jp/c/biotech/index.htm

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 試料中の標的物質を検出又は定量する方法及びキット2012

    • Inventor(s)
      朴 龍洙、董 金華、李 在範、周宏建
    • Industrial Property Rights Holder
      国立大学静岡大学、韓国釜山大学校
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-07-20
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 目的タンパク質の製造方法2012

    • Inventor(s)
      朴 龍洙、金政 真
    • Industrial Property Rights Holder
      国立大学静岡大学
    • Industrial Property Rights Type
      特許
    • Acquisition Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 試料中の標的物質を検出又は定量する方法及びキット2012

    • Inventor(s)
      朴 龍洙、董 金華、李 在範、周 宏建
    • Industrial Property Rights Holder
      静岡大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2012-07-20
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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