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Identification of regeneration-associated genes in zebrafish by a ChIP-on-chip assay and its application to mammals.

Research Project

Project/Area Number 22300109
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionKanazawa University

Principal Investigator

KATO Satoru  金沢大学, 医学系, 教授 (10019614)

Co-Investigator(Renkei-kenkyūsha) TANII Hideji  金沢大学, 医学系, 准教授 (90110618)
Project Period (FY) 2010-04-01 – 2013-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords神経再生分子 / HSP70 / エピジェネティクス / HDACインヒビター / 神経生存 / 軸索伸長 / CHIPアッセイ / 転写因子 / NO / HDAC-2 / S-ニトロシル化 / RARβ / 軸索再伸長 / 魚 / 視神経再生 / 細胞生存 / ラット / ゼブラフィッシュ / Hsp70 / 最初期遺伝子 / 網膜神経節細胞 / 細胞生子
Research Abstract

Regeneration-associated genes (RAGs) were cloned from axotomized zebrafish retina. HSP70 (a stress protein) and purpurin (a retinol-binding protein) were rapidly induced in the fish retina after optic nerve injury. HSP70 was involved in cell survival of injured retinal ganglion cells (RGCs), whereas purpurin was involved in neurite sprouting from injured RGCs. These RAGs could easily induce regrowth of axotomized optic nerve in adult rat.

Report

(4 results)
  • 2013 Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (32 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (18 results) Remarks (2 results)

  • [Journal Article] Neuritogenic activity of trichostatin A in adult rat retinal ganglion cells through acetylation of histone H3 lysine 9 and RAR β induction.2014

    • Author(s)
      Koroyama Y, Sugitani K, Ogai K, Kato S
    • Journal Title

      J. Pharmacol. Sci.

      Volume: 124 Pages: 112-126

    • NAID

      130003382629

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A molecular mechanism of optic nerve regeneration in fish : the retinoid signaling pathway2013

    • Author(s)
      Kato S, Matsukawa T, Koriyama Y, Sugitani K, Ogai K
    • Journal Title

      Prog Retin Eye Res.

      Volume: 37 Pages: 13-30

    • DOI

      10.1016/j.preteyeres.2013.07.004

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Requirement of Retinoic Acid Receptorβfor Genipin Derivative-Induced Optic Nerve Regeneration in Adult Rat Retina2013

    • Author(s)
      Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Sugitani K, Arai K, Suzuki H, Kato S
    • Journal Title

      PLoS One

      Volume: 8 Issue: 8 Pages: e71252-e71252

    • DOI

      10.1371/journal.pone.0071252

    • NAID

      120005327241

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Protective action of nipradilol mediated through S-nitrosylation of Keap1 and HO-1 induction in retinal ganglion cells2012

    • Author(s)
      Koriyama Y
    • Journal Title

      Neurochem. Int.

      Volume: 61 Issue: 7 Pages: 1242-1253

    • DOI

      10.1016/j.neuint.2012.09.004

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Neuritogenic activity of a genipin derivative in retinal ganglion cells is mediated by retinoic acid receptor beta expression through nitric oxide/S-nitrosylation signaling2011

    • Author(s)
      Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Arai K, Matsukawa T, Suzuki H, Sugitani K, Kagechika H, Kato S
    • Journal Title

      J. Neurochem.

      Volume: 119 Issue: 6 Pages: 1232-1242

    • DOI

      10.1111/j.1471-4159.2011.07533.x

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration : Its role in cell survival2011

    • Author(s)
      Nagashima M, Fujikawa C, Mawatari K, Mori Y, Kato S
    • Journal Title

      Neurochem Int.

      Volume: 58 Issue: 8 Pages: 888-895

    • DOI

      10.1016/j.neuint.2011.02.017

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HSP70 gene expression in the zebrafish retina after optic nerve injury : A comparative study under heat shock stresses2011

    • Author(s)
      Fujikawa C
    • Journal Title

      Adv Exp Med Biol

      Volume: 723 Pages: 663-668

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Apoptosis induced by Src-family tyrosine kinase inhibitors in culturedrat cortical cell2011

    • Author(s)
      Takadera T
    • Journal Title

      Neurotox Res

      Volume: 21 Issue: 3 Pages: 309-316

    • DOI

      10.1007/s12640-011-9284-5

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration : Its role in cell survival2011

    • Author(s)
      Mikiko Nagashima
    • Journal Title

      Neurochemistry International.

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 5-S-GAD Attenuates Fe(2+)-induced lipid peroxidation and cell death in a neuronal cell model.2011

    • Author(s)
      Tsuneo Takadera
    • Journal Title

      Neurotoxicity Research

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-acting genipin derivative protects retinal ganglion cells from oxidative stress models in vitro and in vivo through the Nrf2/antioxidant response element signaling pathway2010

    • Author(s)
      Koriyama Y, Chiba K, Yamazaki M, Suzuki H, Muramoto K, Kato S
    • Journal Title

      J. Neurochem.

      Volume: 115 Issue: 1 Pages: 79-91

    • DOI

      10.1111/j.1471-4159.2010.06903.x

    • NAID

      120002561626

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-acting genipin derivative protects retinal ganglion cells from oxidative stress models in vitro and in vivo through the Nrf2/ARE signaling pathway.2010

    • Author(s)
      Yoshiki Koriyama
    • Journal Title

      Journal of Neurochemistry

      Volume: 115 Pages: 79-91

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ゼブラフィッシュ視神経再生における白血病阻止因子(LIF)の発現増加について2014

    • Author(s)
      大貝和裕,杉谷加代,郡山恵樹,加藤聖
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学群元キャンパス
    • Year and Date
      2014-03-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 酸化的ストレス条件下でのマウス網膜におけるニューログロビンの発現とその機能に関する検討2014

    • Author(s)
      杉谷 加代,世良 真悠子,大貝 和裕,郡山 恵樹,若杉 桂輔,加藤 聖
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学群元キャンパス(鹿児島県鹿児島市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 'Upregulation of leukemia inhibitory factor (LIF) during zebrafish optic nerve regeneration2014

    • Author(s)
      大貝 和裕,杉谷 加代,郡山 恵樹,加藤 聖
    • Organizer
      第91回日本生理学会大会
    • Place of Presentation
      鹿児島大学群元キャンパス(鹿児島県鹿児島市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] A novel function of Factor XIII-A in nerve regeneration2013

    • Author(s)
      Sugitani K
    • Organizer
      GTH 2013 (German Trombosis And Heamostasis Society 57th Annual Meeting 2013)
    • Place of Presentation
      ICM (International Congress Center Munish)ドイツミュンヘン
    • Related Report
      2013 Final Research Report
  • [Presentation] Factor XIII as a regeneration associated molecules in fish retina after optic nerve injury.2013

    • Author(s)
      杉谷 加代
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ゼブラフィッシュ視神経損傷後のleukemia inhibitory factor(LIF)とSox2の発現上昇について2013

    • Author(s)
      大貝 和裕,杉谷 加代,郡山 恵樹,中島 廣志,加藤 聖
    • Organizer
      Neuro2013
    • Place of Presentation
      国立京都国際会館(京都市左京区)
    • Related Report
      2012 Annual Research Report
  • [Presentation] A novel function of Factor XIII-A in nerve regeneration2013

    • Author(s)
      Sugitani K
    • Organizer
      GTH2013
    • Place of Presentation
      International Congress Center Munish (ドイツ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 中枢神経系におけるKeap1のS-ニトロシル化による抗酸化活性化機構2012

    • Author(s)
      郡山恵樹
    • Organizer
      第85回日本薬理学会年会
    • Place of Presentation
      国立京都国際会館(京都)
    • Year and Date
      2012-03-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] S-nitrosylation of histone deacetylase 2 promotes optic nerve regeneration by retinoic acid receptor beta expression2012

    • Author(s)
      Koriyama Y, Chiba K, Yamazaki M, Sugitani K, Kato S
    • Organizer
      RD2012
    • Place of Presentation
      Monarch Hotel, Bad Gögging, Germany
    • Related Report
      2013 Final Research Report
  • [Presentation] A distinct effect of two types of transglutaminase on fish optic nerve regeneration2012

    • Author(s)
      Sugitani K, Ogai K, Kato S
    • Organizer
      The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry, The 55th Annual Meeting of the Japanese Society for Neurochemistry合同大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Retinoic acid receptor βinduction by S-nitrosylation of histone deacetylase 2 promotes optic nerve regeneration after injury2012

    • Author(s)
      Koriyama Y, Sugitani K, Chiba K, Karo S
    • Organizer
      The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry, The 55th Annual Meeting of the Japanese Society for Neurochemistry合同大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] S-nitrosylation of histone deacetylase 2 promotes optic nerve regeneration by retinoic acid receptor beta expression2012

    • Author(s)
      Koriyama Y, Chiba K, Yamazaki M, Sugitani K, Kato S
    • Organizer
      RD2012
    • Place of Presentation
      Monarch Hotel (ドイツ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Reciprocal changes of factor XIII and retinal transglutaminase expressions in the fish retina during optic nerve regeneration2012

    • Author(s)
      Sugitani K, Ogai K, Kato S
    • Organizer
      RD2012
    • Place of Presentation
      Monarch Hotel (ドイツ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 視神経損傷後の新規細胞外マトリックス分子プルプリンによるラット網膜神経節細胞の生存と軸索伸長2011

    • Author(s)
      Koriyama Y, Matsukawa T, Nagashima M, Kato S
    • Organizer
      第54回日本神経化学会大会
    • Place of Presentation
      石川県加賀市山代温泉「瑠璃光」
    • Year and Date
      2011-09-28
    • Related Report
      2013 Final Research Report
  • [Presentation] 視神経損傷後の新規細胞外マトリックス分子プルプリンによるラット網膜神経節細胞の生存と軸索伸長2011

    • Author(s)
      郡山恵樹
    • Organizer
      第54回日本神経化学会
    • Place of Presentation
      瑠璃光(石川)
    • Year and Date
      2011-09-28
    • Related Report
      2011 Annual Research Report
  • [Presentation] The expression of KLF4 in zebrafish retina during optic nerve regeneration2010

    • Author(s)
      Maki Nishitani
    • Organizer
      Neuro2010
    • Place of Presentation
      神戸コンベンションセンター(兵庫県)
    • Related Report
      2010 Annual Research Report
  • [Presentation] The earliest inducible molecule in the zebrafish retina for optic nerve regeneration.2010

    • Author(s)
      Chieko Fujikawa
    • Organizer
      RD2010
    • Place of Presentation
      Fairmont Tremblant Hotel(カナダ)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Factor XIIIA induction of regenerating retina and axons in the fish ocular system.2010

    • Author(s)
      Kayo Sugitani
    • Organizer
      RD2010
    • Place of Presentation
      Fairmont Tremblant Hotel(カナダ)
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://neuro.w3.kanazawa-u.ac.jp

    • Related Report
      2013 Final Research Report
  • [Remarks] 金沢大学大学院医学系研究科脳医科学専攻脳細胞分子学講座脳情報分子学

    • URL

      http://neuro.w3.kanazawa-u.ac.jp/

    • Related Report
      2012 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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