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Study on new functions survey of nondigestible oligosaccharide and dietary fiber using accelerated senescence model mouse

Research Project

Project/Area Number 22300263
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Eating habits, studies on eating habits
Research InstitutionUniversity of Nagasaki

Principal Investigator

OKU Tsuneyuki  長崎県立大学, 看護栄養学部, 名誉教授 (50010096)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Sadako  長崎県立大学, 看護栄養学部, 助教 (60382438)
Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Yoshihiko  医療法人宏善会, 諫早記念病院, 病院長 (70253656)
SHIDOJI Yoshihiro  長崎県立大学, 看護栄養学部, 教授 (00111518)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Keywords老化促進マウス / 難消化性オリゴ糖 / 食物繊維 / 腸内フローラ / SAMP マウス / 記憶・行動異常 / 骨代謝 / SAMPマウス / SAMマウス
Research Abstract

The object of the present study was to investigate the cause which the onset of inborn error of metabolism is suppressed, when senescence accelerated mouse-prone 6 and 8 (SAMP6 and SAMP8) were raised on non-digestible oligosaccharide (FOS) and dietary fiber (GM) diets for long period. The composition of diet fed changes intestinal microflora and is transformed the activity of metabolism. As a result, the products of intestinal microbes are changed quantitatively and qualitatively and the existence of host is affected. To clarify the interaction between the change of intestinal microflora and the vital activity of host via intestinal by FOS and GM feeding, the variation of biological markers in concerned with oxidative stress, immune function, and bone metabolism was investigated. In 2012 and 2013, SAMP6 (n=45) with three groups (n=15 each group) and SAMP8 (n=45) with three groups (n=15 each group) were raised on control diet (CONT), FOS diet and GM diet for 32 weeks or 43 weeks, and th … More e biological markers were determined. In SAMP6 fed FOS diet and GM diet, intestinal microbes wereimproved and the bone metabolism became a very excellent. In especially, Bifidobacterium increased markedly and the activity of β-glucronidase and β-galactosidase decreased in FOS group, while in GM group Clostridium increased and the activity of β-glucronidase and β-galactosidase increased. However, the effect for RANKL and 1CTP was different from between FOS group and GM group. These results demonstrate that the function mechanism of FOS and GM are different between FOS and GM. SAMP8 fed FOS diet and GM diet was determined memory and learning disorder and oxidative markers such as BAP test, d-ROMs test, 8-OHdG and isopratan. In FOS group and GM group, intestinal microbes were improved and the senescence of brain was delayed. In addition, anti-oxidative function increased in comparison with that of control group, and inflammatory cytokines were suppressed. In 2013, in focus to brain function, SAMP8 was raised on CONT diet and FOS diet, and SAMR (normal senescence) was raised on CONT diet for 40 weeks to investigate the interaction among the memory and learning disorder, intestinal microbes and brain functions (amyloid-β, catecholamine, brain histochemistry, cytokines, oxidative stress marker s and et al.). The accumulation of amyloid-β in brain was suppressed by FOS ingestion, but the concentration of catecholamine was not changed. Inflammatory cytokineswere decreased by FOS ingestion, and anti-inflammatory cytokines were increased. The oxidative stress markers such as Bap test, d-Roms test, 8-OHdG and isoprastan were improved by FOS ingestion. These results demonstrate that long-term intake of FOS and GM improves intestinal microbes and also suppress or delay the learning and memory disorder and the aging in SAMP8. Thus, the suppression of oxidative stress and deposition of amyloid-β through intestinal microflora is concerned with senescenceand brain function. Less

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (31 results)

All 2013 2012 2011

All Presentation (31 results)

  • [Presentation] Effects of long-term feeding with non-digestible oligosaccharide and dietary fiber on age-related learning and memory of senescence accelerated mice2013

    • Author(s)
      ○Yamaguchi Y ; Kondo N; Tanabe K; Nakamura S; Oku T
    • Organizer
      20th Inter Cong Nutr
    • Place of Presentation
      Granada, Spain
    • Year and Date
      2013-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP8 における多尿ならびに高血糖の発症とその要因の検討2013

    • Author(s)
      中村禎子,近藤直幸,山口喜勇,田辺賢一, 奥 恒行
    • Organizer
      老化促進モデルマウス研究協議会
    • Place of Presentation
      愛知学院大学
    • Year and Date
      2013-07-05
    • Related Report
      2012 Final Research Report
  • [Presentation] フラクトオリゴ糖摂取が腸内細菌叢の改善を介して老化促進マウス SAMP6 の酸化ストレスならびに骨代謝に及ぼす影響2013

    • Author(s)
      ○田辺賢一,山口喜勇,中村禎子,奥 恒行
    • Organizer
      老化促進モデルマウス研究協議会
    • Place of Presentation
      愛知学院大学
    • Year and Date
      2013-07-05
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP8 の加齢に伴う学習・記憶および情動と脳機能因子に及ぼす難消化性オリゴ糖継続摂取の影響2013

    • Author(s)
      山口喜勇,後田ちひろ,宮原明美,中村禎子,田辺賢一,奥 恒行
    • Organizer
      日本栄養・食糧学会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2013-05-24
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウスSAMP8の加齢に伴う学習・記憶および情動と脳機能因子に及ぼす難消化性オリゴ糖継続摂取の影響2013

    • Author(s)
      山口喜勇,後田ちひろ,宮原明美,中村禎子,田辺賢一,奥 恒行
    • Organizer
      平成25年度日本栄養・食糧学会
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 老化促進マウスSAMP8における多尿ならびに高血糖の発症とその要因の検討2013

    • Author(s)
      中村禎子,近藤直幸,山口喜勇,田辺賢一,奥 恒行
    • Organizer
      老化促進モデルマウス研究協議会
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Annual Research Report
  • [Presentation] フラクトオリゴ糖摂取が腸内細菌叢の改善を介して老化促進マウスSAMP6の酸化ストレスならびに骨代謝に及ぼす影響2013

    • Author(s)
      田辺賢一,山口喜勇,中村禎子,奥 恒行
    • Organizer
      老化促進モデルマウス研究協議会
    • Place of Presentation
      名古屋市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effects of long-term feeding with non-digestible oligosaccharide and dietary fiber on age-related learning and memory of senescence accelerated mice2013

    • Author(s)
      Yamaguchi Y; Kondo N; Tanabe K; Nakamura S; Oku T
    • Organizer
      20th International Congress of Nutrition
    • Place of Presentation
      Granada, Spain
    • Related Report
      2012 Annual Research Report
  • [Presentation] 老化促進マウス SAMP8 の学習・記憶能力ならびに脳機能因子に及ぼす難消化性オリゴ糖および食物繊維の継続摂取の影響2012

    • Author(s)
      山口喜勇,中村禎子,田辺賢一,後田ちひろ,近藤直幸,奥 恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      中村学園大学(福岡市)
    • Year and Date
      2012-11-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP6 の骨粗鬆症発症に及ぼす難消化性オリゴ糖および食物繊維長期摂取の影響2012

    • Author(s)
      田辺賢一,北島美穂、中村禎子,奥 恒行
    • Organizer
      日本栄養・食糧学会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2012-05-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP8 の加齢に伴う脳機能因子に及ぼす難消化性オリゴ糖および食物繊維継続摂取の影響2012

    • Author(s)
      ○山口喜勇,近藤直幸,橋口美智留、田辺賢一,中村禎子,奥 恒行
    • Organizer
      日本栄養・食糧学会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2012-05-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウスSAMP8の学習・記憶能力ならびに脳機能因子に及ぼす難消化性オリゴ糖および食物繊維の継続摂取の影響2012

    • Author(s)
      山口喜勇,中村禎子,田辺賢一,後田ちひろ,近藤直幸,奥 恒行
    • Organizer
      第17回日本食物繊維学会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 腸内細菌を介した難消化吸収性単糖・オリゴ糖の生体利用の相違2011

    • Author(s)
      中村禎子、福嶋恭子、奥 恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所(東京都)
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP6 の骨代謝に及ぼす難消化性糖質の影響2011

    • Author(s)
      田辺賢一,森山美枝,中村禎子,奥 恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所(東京都)
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP6 の生体防御に及ぼす難消化性糖質の影響2011

    • Author(s)
      江島寛幸,田辺賢一,中村禎子,奥 恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所(東京都)
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP8 の記憶・行動異常に及ぼす難消化性糖質の影響2011

    • Author(s)
      近藤直幸,田辺賢一,中村禎子,奥 恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所(東京都)
    • Year and Date
      2011-11-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP8 の腸内フローラに及ぼす難消化性糖質の影響2011

    • Author(s)
      高見昌之,田辺賢一,中村禎子,奥 恒行
    • Organizer
      日本栄養・食糧学会九州・沖縄支部大会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Year and Date
      2011-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP8 の腸内細菌生成物に及ぼす難消化性糖質の影響2011

    • Author(s)
      江島寛幸、田辺賢一,中村禎子,奥 恒行
    • Organizer
      日本栄養・食糧学会九州・沖縄支部大会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Year and Date
      2011-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウス SAMP8 の記憶・行動に及ぼす難消化性糖質の影響2011

    • Author(s)
      近藤直幸、中村禎子、奥 恒行
    • Organizer
      日本栄養・食糧学会九州・沖縄支部大会
    • Place of Presentation
      佐賀大学 (佐賀市)
    • Year and Date
      2011-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 老化促進マウスSAMP6 の各種生体指標に及ぼす難消化性糖質の影響2011

    • Author(s)
      江島寛幸、伊森千晃、福嶋恭子、田辺賢一、中村禎子、奥 恒行
    • Organizer
      日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大(東京都)
    • Year and Date
      2011-05-14
    • Related Report
      2012 Final Research Report
  • [Presentation] ラットにおけるα-グルコシダーゼ阻害物質と同時に摂取した消化性糖質のプレバイオティクス効果2011

    • Author(s)
      中村禎子, 伊森千晃, 橋口美智留, 田辺賢一, 奥恒行
    • Organizer
      日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 老化促進マウスSAMP6の各種生体指標に及ぼす難消化性糖質の影響2011

    • Author(s)
      江島寛幸, 伊森千晃, 福嶋恭子, 田辺賢一, 中村禎子, 奥恒行
    • Organizer
      日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大
    • Year and Date
      2011-05-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] ラットにおける α-グルコシダーゼ阻害物質と同時に摂取した消化性糖質のプレバイオティクス効果2011

    • Author(s)
      中村禎子,伊森千晃,橋口美智留,田辺賢一,奥 恒行
    • Organizer
      日本栄養・食糧学会
    • Place of Presentation
      お茶の水女子大(東京都)
    • Year and Date
      2011-05-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 難消化性糖質の継続摂取が老化促進マウスSAMP6の骨代謝に及ぼす影響2011

    • Author(s)
      田辺賢一, 中村禎子, 奥恒行
    • Organizer
      日本栄養・食糧学会九州・冲縄支部大会
    • Place of Presentation
      佐賀大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 老化促進マウスSAMP8の腸内細菌生成物に及ぼす難消化性糖質の影響2011

    • Author(s)
      江島寛幸, 田辺賢一, 中村禎子, 奥恒行
    • Organizer
      日本栄養・食糧学会九州・沖縄支部大会
    • Place of Presentation
      佐賀大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 老化促進マウスSAMP8の腸内フローラに及ぼす難消化性糖質の影響2011

    • Author(s)
      高見昌之, 田辺賢一, 中村禎子, 奥恒行
    • Organizer
      日本栄養・食糧学会九州・沖縄支部大会
    • Place of Presentation
      佐賀大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 老化促進マウスSAMP8の記憶・行動に及ぼす難消化性糖質の影響2011

    • Author(s)
      近藤直幸, 中村禎子, 奥恒行
    • Organizer
      日本栄養・食糧学会九州・沖縄支部大会
    • Place of Presentation
      佐賀大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腸内細菌を介した難消化吸収性単糖・オリゴ糖の生体利用の相違2011

    • Author(s)
      中村禎子, 福嶋恭子, 奥恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所
    • Related Report
      2011 Annual Research Report
  • [Presentation] 老化促進マウスSAMP6の骨代謝に及ぼす難消化性糖質の影響2011

    • Author(s)
      田辺賢一, 森山美枝, 中村禎子, 奥恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所
    • Related Report
      2011 Annual Research Report
  • [Presentation] 老化促進マウスSAMP6の生体防御に及ぼす難消化性糖質の影響2011

    • Author(s)
      江島寛幸, 田辺賢一, 中村禎子, 奥恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所
    • Related Report
      2011 Annual Research Report
  • [Presentation] 老化促進マウスSAMP8の記憶・行動異常に及ぼす難消化性糖質の影響2011

    • Author(s)
      近藤直幸, 田辺賢一, 中村禎子, 奥恒行
    • Organizer
      日本食物繊維学会
    • Place of Presentation
      国立健康・栄養研究所
    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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