Significance and Molecular mechanisms of abnormal expression of miRNA in carcinogenesis.
Project/Area Number |
22300318
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Carcinogenesis
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Research Institution | The University of Tokyo |
Principal Investigator |
IBA Hideo 東京大学, 医科学研究所, 教授 (60111449)
|
Co-Investigator(Kenkyū-buntansha) |
HARAGUCHI Takeshi 東京大学, 医科学研究所, 助教 (10549455)
KOHARA Michinori 東京都医学総合研究所, 副参事研究員 (10250218)
INADA Kenichi 藤田保健衛生大学, 医学部, 准教授 (70246081)
MIZUTANI Taketoshi 東京大学, 医科学研究所, 助教 (00376617)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
|
Keywords | 発現制御 / 上皮‐間充織変換 / Cdx1 / miR-200c / miR-199a / miR-15/-16 / Brm / 上皮-間充織変換 / miR200c / miRNA / SWI/SNF複合体 / フィードバックループ / がん細胞 / Cdx2 / CD44 / エピジェネティクス / Egr1 |
Research Abstract |
There have been reported to be about 1000 species of miRNA in human, among them, such miRNAs as miR-21, miR-199a, miR-15/-16 and miR-200c are expressed at aberrant levels in cancer tissues. In this research, we demonstrated how these aberrant expression were induced and maintained, and found in several cases, stable gene regulatory net works have been formed among the miRNA, transcription factors and SWI/SNF chromatin remodeling factors. We further show the RNA decoy molecules that we developed for the inhibitor of specificmicorRNA activity (designated TuD) is very effective for the analysis of molecular mechanisms and for future therapietic application for cancer.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Multiple microRNAs induced by Cdx1 suppress Cdx2 in human colorectal tumor cells.2012
Author(s)
Tagawa, T., Haraguchi, T., Hiramatsu, H., Kobayashi, K., Sakurai, K., Inada, K., and Iba, H.
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Journal Title
Biochem. J.
Volume: 447
Pages: 449-455
Related Report
Peer Reviewed
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[Journal Article] Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors2012
Author(s)
Y. Kurashima, T. Amiya, T. Nochi, K. Fujisawa, T. Haraguchi, H. Iba, H. Tsutsui, S. Sato, S. Nakajima, H. Iijima, M. Kubo, J. Kunisawa, H. Kiyono
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Journal Title
Nat Commun.
Volume: 3-1034
Issue: 1
Pages: 1034-1034
DOI
Related Report
Peer Reviewed
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[Journal Article] Double plant homeodomain (PHD) finger proteins DPF3a and -3b are required as transcriptional co-activators in SWI/SNF complex-dependent activation of NF- RelA/p50 heterodimer.2012
Author(s)
Ishizaka, A., Mizutani, T., Kobayashi, K., Tando, T., Sakurai, K., Fujiwara, T., and Iba, H.
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Journal Title
J. Biol. Chem.
Volume: 287
Pages: 11924-11933
Related Report
Peer Reviewed
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[Journal Article] A potent2'-O-methylated RNA-based microRNAinhibitor with unique secondary structures.2012
Author(s)
Haraguchi, T., Nakano, H., Tagawa, T., Ohki, T., Ueno, Y., Yoshida, T., and Iba, H.
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Journal Title
Nucleic Acids Res.
Volume: 40
Related Report
Peer Reviewed
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[Journal Article] A potent 2'-O-methylated RNA-based microRNA inhibitor with unique secondary structures2012
Author(s)
Haraguchi,T., Nakano, H., Tagawa, T., Ohki, T., Ueno, Y., Yoshida, T., Iba, H.
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Journal Title
Nucleic Acids Res.
Volume: (in press)
Issue: 8
Pages: 1-13
DOI
Related Report
Peer Reviewed
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[Journal Article] MicroRNA regulation of glycoprotein B5R in oncolytic vaccinia virus reduces viral pathogenicity without impairing its antitumor efficacy.2011
Author(s)
Hikichi, M., Kidokoro, M., Haraguchi,T., Iba, H., Shida, H., Tahara, H., and Nakamura, T.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] microRNAs miR-199a-5p and -3p target the Brm subunit of SWI/SNFto generate a double-negative feedback loop ina variety of human cancers.2011
Author(s)
Sakurai, K., Furukawa, C., Haraguchi, T., Inada, K.,Shiogama, K., Tagawa, T., Fujita, S., Ueno, Y., Ogata, A., Ito,M., Tsutsumi, Y., and Iba, H.
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Journal Title
Cancer Research
Volume: 71
Pages: 1680-1689
Related Report
Peer Reviewed
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[Journal Article] Incorporation of biaryl units into the 5′ and3′ ends of sense and antisense strands of siRNA duplexes improves strand selectivity and nuclease resistance.2011
Author(s)
Yoshikawa, K., Ogata, A., Matsuda, C., Kohara, M., Iba, H., Kitade, Y., and Ueno, Y.
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Journal Title
Bioconjug. Chem.
Volume: 22
Pages: 42-49
Related Report
Peer Reviewed
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[Journal Article] MicroRNA regulation of glycoprotein B5R in oncolytic vaccinia virus reduces viral pathogenicity without impairing its antitumor efficacy2011
Author(s)
Hikichi, M., Kidokoro, M., Haraguchi, T., Iba, H., Shida, H., Tahara, H., Nakamura, T.
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Journal Title
Molecular Therapy
Volume: 19
Pages: 1107-1115
Related Report
Peer Reviewed
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[Journal Article] Incorporation of biaryl units into the 5' and 3' ends of sense and antisense strands of siRNA duplexes improves strand selectivity and nuclease resistance.2011
Author(s)
Yoshikawa, K., Ogata, A., Matsuda, C., Kohara, M., Iba, H., Kitade, Y., Ueno, Y.
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Journal Title
Bioconjug.Chem.
Volume: 22
Pages: 42-49
Related Report
Peer Reviewed
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[Journal Article] MicroRNA regulation of glycoprotein B5R in oncolytic vaccinia virus reduces viral pathogenicity without impairing its antitumor efficacy.2011
Author(s)
Hikichi, M., Kidokoro, M., Haraguchi, T., Iba, H., Shida, H., Tahara, H., Nakamura, T.
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Journal Title
Molecular Therapy
Volume: (Published online)
Related Report
Peer Reviewed
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[Journal Article] Biarylmodification of the 5'-terminus of one strandof a microRNA duplex induces strand specificity.2010
Author(s)
Ogata, A., Furukawa, C., Sakurai, K., Iba, H., Kitade, Y., and Ueno, Y.
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Journal Title
Bioorg. Med. Chem. Lett.
Volume: 20
Pages: 7299-7302
Related Report
Peer Reviewed
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[Journal Article] Requiem protein links RelB/p52 and theBrm-type SWI/SNF complex in a non-canonical NFκB pathway.2010
Author(s)
Tando, T., Ishizaka, A., Watanabe, H., Ito, T., Iida, S., Haraguchi, T., Mizutani, T., Izumi, T., Isobe, T., Akiyama, T., Inoue, J., and Iba, H.
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Journal Title
J. Biol. Chem.
Volume: 285
Pages: 21951-21960
Related Report
Peer Reviewed
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[Journal Article] Requiem protein links RelB/p52 and the Brm-type SWI/SNF complex in a non-canonical NF_KB pathway.2010
Author(s)
Tando, T., Ishizaka, A., Watanabe, H., Ito, T., Iids, S., Haraguchi, T., Mizutani, T., Izumi, T., Isobe, T., Akiyama, T., Inoue, J., Iba, H.
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Journal Title
J.Biol.Chem.
Volume: 285
Pages: 21951-21960
Related Report
Peer Reviewed
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[Journal Article] Biaryl modification of the 5'-terminus of one strand of a microRNA duplex induces strand specificity.2010
Author(s)
Ogata, A., Furukawa, C., Sakurai, K., Iba H., Kitade, Y., Ueno, Y.
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Journal Title
Bioorg.Med.Chem.Lett.
Volume: 20
Pages: 7299-7302
Related Report
Peer Reviewed
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[Journal Article] microRNAs miR-199a-5p and -3p target the Brm subunit of SWI/SNF to generate a double-negative feedback loop in a variety of human cancers.2010
Author(s)
Sakurai, K., Furukawa, C., Haraguchi, T., Inada, K., Shiogama, K., Tagawa, T., Fujita, S., Ueno, Y., Ogata, A., Ito, M., Tsutsumi, Y., Iba, H.
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Journal Title
Cancer Research
Volume: 71
Pages: 1680-1689
Related Report
Peer Reviewed
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