Functional analysis of mouse noncoding RNAs by a rapid gene disruption method
Project/Area Number |
22310118
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genome biology
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Research Institution | Nara Medical University (2012-2013) Osaka University (2010-2011) |
Principal Investigator |
HORIE Kyoji 奈良県立医科大学, 医学部, 教授 (30333446)
|
Project Period (FY) |
2010-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2012: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | ES細胞 / ゲノム / 遺伝子 / non-coding RNA / 変異体 / non-oding RNA |
Outline of Final Research Achievements |
Recent researches demonstrated that most regions of the mammalian genome such as human and mouse are transcribed. Furthermore, most of such transcripts are noncoding RNAs. However, speculation of the function of noncoding RNAs from nucleotide sequences is difficult. We recently developed a method to rapidly generate mutant mouse ES cells. In the present study, we generated mutant mouse ES cell lines using this method and conducted systematic functional analysis of noncoding RNAs.
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Report
(5 results)
Research Products
(14 results)
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[Journal Article] Removal of reprogramming transgenes improves the tissue reconstitution potential of keratinocytes generated from human induced pluripotent stem cells2014
Author(s)
Ken Igawaa, Chikara Kokubu, Kosuke Yusa, Kyoji Horie, Yasuhide Yoshimura, Kaori Yamauchi, Hirofumi Suemori, Hiroo Yokozeki, Masashi Toyoda, Nobutaka Kiyokawa, Hajime Okita, Yoshitaka Miyagawa, Hidenori Akutsu, Akihiro Umezawa, Ichiro Katayama, Junji Takeda.
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Journal Title
Stem Cells Transl Med
Volume: 3
Issue: 9
Pages: 992-1001
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] An in vitro ES cell-based clock recapitulation assay model identifies CK2a as an endogenous clock regulator2013
Author(s)
Umemura Y, Yoshida J, Wada M, Tsuchiya Y, Minami Y, Watanabe H, Kondoh G, Takeda J, Inokawa H, Horie K, Yagita K.
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Journal Title
PLoS One
Volume: 8
Issue: 6
Pages: e67241-e67241
DOI
Related Report
Peer Reviewed / Open Access
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