Analysis of the outer membrane BAM complex
Project/Area Number |
22370044
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Structural biochemistry
|
Research Institution | Yokohama City University |
Principal Investigator |
J・R・H Tame 横浜市立大学, 生命医科学研究科, 教授 (00336588)
|
Co-Investigator(Kenkyū-buntansha) |
PARK Sam-yong 横浜市立大学, 生命医科学研究科, 教授 (20291932)
UNZAI Satoru 横浜市立大学, 生命医科学研究科, 助教 (60336592)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2010: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
|
Keywords | 膜タンパク質 / 抗生剤 / 結晶 / タンパク質 / X線結晶構造解析 / 細胞膜輸送 / オートトランスポーター |
Research Abstract |
Gram negative bacteria require the BAM complex for the insertion of membrane proteins into the outer membrane. This essential complex consists of five proteins, the large transmembrane barrel being BamA. BamA carries five POTRA domains at its N terminus which interact with other proteins in the complex. Only BamA and BamD are essential, but the roles of these proteins is unknown. We have solved the crystal structure of an outer membrane protein, Hbp, and studied the interaction between BamC and BamD. The Hbp beta-barrel is a substrate of BAM, but the interactions between the two are unclear. We have shown that BamC and BamD interact to give a flexible complex.
|
Report
(4 results)
Research Products
(38 results)
-
-
-
[Journal Article] Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle2012
Author(s)
Hansman, G. S., Taylor, D. W., McLellan,J. S., Smith, T. J., Georgiev, I., Tame, J. R. H., Park, S. Y., Yamazaki, M., Gondaira, F., Miki, M., Katayama, K., Murata, K. & Kwong, P. D
-
Journal Title
J Virol
Volume: 86
Pages: 3635-46
Related Report
-
-
[Journal Article] Structures of haemoglobin from woolly mammoth in liganded and unliganded states2012
Author(s)
Noguchi, H., Campbell, K. L., Ho, C., Unzai, S., Park, S. Y. & Tame, J. R. H
-
Journal Title
Acta Crystallogr D Biol Crystallogr
Volume: 68
Pages: 1441-9
Related Report
-
[Journal Article] Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms2012
Author(s)
Yoshida, H., Kawai, F., Obayashi, E., Akashi, S., Roper, D. I., Tame, J. R. H. & Park, S. Y.
-
Journal Title
J Mol Biol
Volume: 423
Pages: 351-64
Related Report
-
-
[Journal Article] Structures of haemoglobin from woolly mammoth in liganded and unliganded states.2012
Author(s)
Noguchi, H., Campbell, K. L., Ho, C., Unzai, S., Park, S. Y. & Tame, J. R. H.
-
Journal Title
Acta Crystallogr D Biol Crystallogr
Volume: 412
Issue: 11
Pages: 551-2
DOI
Related Report
Peer Reviewed
-
[Journal Article] PLoS One2012
Author(s)
Matsunaga, Y., Koike, R., Ota, M., Tame, J. R. H. & Kidera, A.
-
Journal Title
Influence of structural symmetry on protein dynamics.
Volume: 7
Issue: 11
Pages: 11-11
DOI
Related Report
Peer Reviewed
-
[Journal Article] Structural Basis for Broad Detection of Genogroup II Noroviruses by a Monoclonal Antibody That Binds to a Site Occluded in the Viral Particle2012
Author(s)
Hansman, G.S., Taylor, D.W., McLellan, J.S., Smith, T.J., Georgiev, I., Tame, J.R., Park, S.Y., Yamazaki, M., Gondaira, F., Miki, M., Katayama, K., Murata, K., Kwong, P.D.
-
Journal Title
Journal of Virology
Volume: 86(7)
Issue: 7
Pages: 3635-3646
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Substitutions in woolly mammoth hemoglobin confer biochemical properties adaptive for cold tolerance2010
Author(s)
Campbell, K. L., Roberts, J. E., Watson, L. N., Stetefeld, J., Sloan, A. M., Signore, A. V., Howatt, J. W., Tame, J. R. H.,Rohland, N., Shen, T. J., Austin, J. J., Hofreiter, M., Ho, C., Weber, R. E. & Cooper, A
-
Journal Title
Nat Genet
Volume: 42
Pages: 536-40
Related Report
-
-
[Journal Article] Role of domains within the autotransporter Hbp/Tsh2010
Author(s)
Nishimura, K., Yoon, Y. H., Kurihara, A., Unzai, S., Luirink, J., Park, S. Y. & Tame, J. R. H
-
Journal Title
Acta Crystallogr D Biol Crystallogr
Volume: 66
Pages: 1295-300
Related Report
-
[Journal Article] Order and disorder in the domain organization of the plasmid partition protein KorB2010
Author(s)
Rajasekar, K., Muntaha, S. T., Tame, J. R. H., Kommareddy, S., Morris, G., Wharton, C. W., Thomas, C. M., White, S. A., Hyde, E. I. & Scott, D. J
-
Journal Title
J Biol Chem
Volume: 285
Pages: 15440-9
Related Report
-
[Journal Article] A conserved aromatic residue in the autochaperone domain of the autotransporter Hbp is critical for initiation of outer membrane translocation2010
Author(s)
Soprova, Z., Sauri, A., van Ulsen, P., Tame, J. R. H., den Blaauwen, T., Jong, W. S. & Luirink, J.
-
Journal Title
J Biol Chem
Volume: 285
Pages: 38224-33
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-