Identification and biochemical characterization of RNA ligasesin archaea
| Project/Area Number |
22370066
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Keio University |
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Principal Investigator |
KANAI Akio 慶應義塾大学, 環境情報学部, 教授 (60260329)
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| Research Collaborator |
慶應義塾大学, 大学院, 学生
慶應義塾大学, 先端生命科学研究所, 技術員
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
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| Keywords | RNA連結 / RNAリガーゼ / 超好熱性アーキア / 再構成系 / tRNA / アーキア / 生化学 / 分子生物学 / プロテアーゼ / トランスポゾン / 複合体 / Pyrococcus furiosus / RNA ligase / RNAプロセシング / イントロン / tRNA前駆体 / 組換え体酵素 |
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| Research Abstract |
RNA ligase is an enzyme that can catalyze the ligation of RNA molecules throughphosphodiester bond. We cloned three RNA ligase genes from a hyperthermophilic archaeon,P. furiosus and characterized the recombinant proteins. The three enzymes are: (1) aputa
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Genomic heterogeneity in a naturalarchaeal population suggests a modelof tRNA gene disruption2012
Author(s)
Sugahara, J., Fujishima, K., Nunoura,T., Takaki, Y., Takami, H., Takai, K.,Tomita, M. and Kanai, A
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Journal Title
DOI
Related Report
Peer Reviewed
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[Journal Article] Nematode-specific tRNAsthat decode an alternative geneticcode for leucine.2012
Author(s)
Hamashima, K., Fujishima, K., Masuda,T., Sugahara, J., Tomita, M. and Kanai,A.
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Journal Title
Nucleic Acids Research
Volume: 40(8)
Pages: 3653-3662
DOI
Related Report
Peer Reviewed
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[Journal Article] A novelthree-unit tRNA splicingendonuclease found in ultrasmallarchaea possesses broad substratespecificity.2011
Author(s)
Fujishima, K., Sugahara, J., Miller,CS., Baker BJ., Di Giulio, M., Takesue,K., Sato, A., Tomita, M., Banfield, JF.and Kanai, A.
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Journal Title
Nucleic Acids Research
Volume: 39(22)
Pages: 9695-9704
DOI
Related Report
Peer Reviewed
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[Journal Article] GTP-dependent RNA 3'-terminalphosphate cyclase from thehyperthermophilic archaeonPyrococcus furiosus.2011
Author(s)
Sato, A., Soga, T., Igarashi, K.,Takesue, K., Tomita, M. and Kanai, A
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Journal Title
Genes to Cells
Volume: 16
Pages: 1190-1199
DOI
Related Report
Peer Reviewed
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[Journal Article] Insights into the evolution ofarchaea and eukaryotic proteinmodifier systems reveled by thegenome of a novel archaeal group2011
Author(s)
Nunoura, T., Takaki, Y., Kakuta, J.,Nishi, S., Sugahara, J., Kazama, H.,Chee, G.-J., Hattori, M., Kanai, A.,Atomi, H., Takai, K. and Takami, H.
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Journal Title
Nucleic Acids Research
Volume: 39 (8)
Pages: 3204-3223
DOI
Related Report
Peer Reviewed
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