Mechanisms of blastema formation after leg amputationin Gryllus bimaculatus

• Project/Area Number: 22370080
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: The University of Tokushima
• Principal Investigator: NOJI Sumihare 徳島大学, 本部, 理事 (40156211)
• Co-Investigator(Kenkyū-buntansha): OHUCHI Hideyo 岡山大学, 医歯薬学総合研究科, 教授 (00253229) ; MITO Taro 徳島大学, 大学院・ソシオテクノサイエンス研究部, 助教 (80322254)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: コオロギ / 脚再生 / ゲノム編集技術 / Jak/Stat 系 / ノックアウトコオロギ / RNAi / エピジェネティク / Jak/Stat系 / TALEN / ZFN / ゲノム編集 / Jak / Stat / ヒートショック / シスエレメント / 発現解析 / 再生芽形成 / Enhancer of Zeste / エピジェネティックス / ヒストン修飾因子

Research Abstract

We attempted to elucidate molecular mechanisms underlying leg regeneration of the cricket nymph. We obtained cricket genome data, using new sequencers to know basic genetic data. We also developed a new method to edit cricket genome by artificial nucleases such as TALEN or CRISPR/Cas. We made successfully knockout crickets. We found that the Jak/Stat signaling system is involved in formation of blastema. With our results, we may facilitate elucidation of regeneration mechanisms in general

Research Products

• [Journal Article] Analysis of RNA-Seq data reveals involvement of JAK/STAT signalling during leg regeneration in the cricket Gryllus bimaculatus.Development (2013)
  • Author(s): Bando T, Ishimaru Y, Kida T, Hamada Y, Matsuoka Y, Nakamura T, Ohuchi H, Noji S, Mito T
  • Journal Title: Development Volume: 140 Pages: 959-964
  • DOI: 10.1242/dev.084590
  • Peer Reviewed
• [Journal Article] Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases. (2012)
  • Author(s): Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T.
  • Journal Title: Nat. Commun. Volume: 3 Pages: 1017-1017
  • DOI: 10.1038/ncomms2020
  • Peer Reviewed
• [Journal Article] Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus (2012)
  • Author(s): Takagi A, Kurita K, Terasawa T, Nakamura T, Bando T, Moriyama Y, Mito T, Noji S, Ohuchi H
  • Journal Title: Dev Growth Differ Volume: 54 Pages: 227-240
  • NAID: 10030405748
  • Peer Reviewed
• [Journal Article] Functional analysis of the role of eyes absent and sine oculis in the developing eye of the cricket Gryllus bimaculatus (2012)
  • Author(s): Takagi, A., et al
  • Journal Title: Develop. Growth Differ. Volume: 54 Pages: 227-240
  • DOI: 10.1111/j.1440-169x.2011.01325.x
  • NAID: 10030405748
  • Peer Reviewed
• [Journal Article] Ancestral functions of Delta/Notch signaling in the formation of body and leg segments in the cricket Gryllus bimaculatus (2011)
  • Author(s): Mito, T., et al
  • Journal Title: Development. Volume: 138 Pages: 3823-3833
  • DOI: 10.1242/dev.060681
  • Peer Reviewed
• [Journal Article] Cricket body size is altered by systemic RNAi against insulin signaling components and epidermal growth factor receptor (2011)
  • Author(s): Dabour N, Bando T, Nakamura T, Miyawaki K, Mito T, Ohuchi H, Noji S
  • Journal Title: Dev Growth Di Volume: 53 Pages: 857-869
  • NAID: 10030403594
  • Peer Reviewed
• [Journal Article] Lowfat, a mammalian Lix1 homologue, regulates leg size and growth under the Dachsous/Fat signaling pathway during tissue regeneration. (2011)
  • Author(s): Bando Tetsuya
  • Journal Title: Dev Dyn Volume: 240(6) Pages: 1440-1453
  • DOI: 10.1002/dvdy.22647
  • Peer Reviewed
• [Journal Article] Regulation of leg size and shape: involvement of the Dachsous-fat signaling pathway. (2011)
  • Author(s): Bando Tetsuya
  • Journal Title: Dev. Dyn Volume: 240(5) Pages: 1028-1041
  • DOI: 10.1002/dvdy.22590
  • Peer Reviewed
• [Journal Article] Imaging of transgenic cricket embryos reveals cell movements consistent with a syncytial patterning mechanism (2010)
  • Author(s): Nakamura T, Yoshizaki M, Ogawa S, Okamoto H, Shinmyo Y, Bando T, Ohuchi H, Noji S, Mito T
  • Journal Title: Curr. Biol Volume: 18 Pages: 1641-1647
  • DOI: 10.1016/j.cub.2010.07.044
  • Peer Reviewed
• [Presentation] Dachsous/Fat signaling via Hippo/Salvador/Warts pathway regulates cell proliferation and pattern formation during leg regeneration in the cricket. (2012)
  • Author(s): T. Bando
  • Organizer: XXIV International Congress of Entomology
  • Place of Presentation: The EXCO-Daegu Convention Center (Daegu, Korea)
  • Invited
• [Presentation] Molecular mechanisms underlying insect leg regeneration : from wound healing to leg size determination (2011)
  • Author(s): Noji, S
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)(招待講演)
  • Year and Date: 2011-12-16
• [Presentation] Regeneration of Insect Legs : Involvement of the JAK-STAT and Dachsous-Fat Signaling Pathways (2011)
  • Author(s): Noji, S
  • Organizer: The 1st CDB-Regeneration Biology Study Group meeting "from Regeneration Biology to Regenerative Medicine (T)"
  • Place of Presentation: 理化学研究所発生再生センター(神戸市)(招待講演)
  • Year and Date: 2011-11-25
• [Presentation] Spatio-temporally controlled misexpression of genes using the GAL4/UAS system in the cricket, Gryllus bimaculatus (2011)
  • Author(s): Matsuda, K.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-20
• [Presentation] Targeted manipulation of genes with zinc finger nucleases in the cricket, Gryllus bimaculatus (2011)
  • Author(s): Watanabe, T.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄コンベンションセンター(沖縄県)
  • Year and Date: 2011-05-19
• [Presentation] コオロギの脚再生中のDachsous/Fatシグナル経路によるサイズと成長の制御 (2010)
  • Author(s): 濱田良真, 板東哲哉, 中村太郎, 三戸太郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07