Gene mapping and physiological characterization of leaf starch excess mutants of rice

• Project/Area Number: 22380017
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: HIROSE Tatsuro 独立行政法人農業・食品産業技術総合研究機構, 中央農業総合研究センター作物開発研究領域, 主任研究員 (90355579)
• Co-Investigator(Kenkyū-buntansha): OHSUGI Ryu 東京大学, 農学生命科学研究科, 教授 (40343107) ; AOKI Naohiro 東京大学, 農学生命科学研究科, 助教 (70466811)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2010: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: イネ / 遺伝子 / デンプン / 転流 / 突然変異体

Research Abstract

To elucidate the molecular mechanisms of sugar translocation and metabolism, five lines of rice mutant that accumulate high levels of leaf starch were characterized. In one mutant line named LSE1, disruption of a gene for glucan water dikinase was revealed to be a responsible gene. Gene mapping and physiological characterization were done also in other four mutant lines.

Research Products

• [Journal Article] Disruption of a rice gene for α-glucan water dikinase, OsGWD1, leads to hyperaccumulation of starch in leaves but exhibits limited effects on growth (2013)
  • Author(s): Tatsuro Hirose, Naohiro Aoki, YusukeHarada, Masaki Okamura, Yoichi Hashida,Ryu Ohsugi, Akio Miyao, Hirohiko Hirochika, Tomio Terao
  • Journal Title: Frontiers Plant Sci. Volume: Vol.4 Pages: 143-143
  • DOI: 10.3389/fpls.2013.00147
  • Peer Reviewed
• [Presentation] イネ葉中デンプン過剰蓄積変異体の生理機能解析と原因遺伝子の単離 (2012)
  • Author(s): 廣瀬竜郎、原田祐介、青木直大、大杉立、宮尾安藝雄、廣近洋彦、寺尾富夫
  • Organizer: 日本作物学会第233回講演会
  • Place of Presentation: 東京都府中市
  • Year and Date: 2012-03-30
• [Presentation] イネ葉中デンプン過剰蓄積変異体の生理機能解析と原因遺伝子の単離 (2012)
  • Author(s): 廣瀬竜郎
  • Organizer: 日本作物学会第233回講演会
  • Place of Presentation: 東京農工大学農学部(東京都)
  • Year and Date: 2012-03-30
• [Presentation] イネ葉中デンプン過剰蓄積変異体の生理特性の解析 (2011)
  • Author(s): 原田祐介、廣瀬竜郎、寺尾富夫、宮尾安藝雄、廣近洋彦、青木直大、大杉立
  • Organizer: 日本作物学会第231回講演会
  • Place of Presentation: 神奈川県厚木市
  • Year and Date: 2011-03-31
• [Presentation] イネ葉中デンプン過剰蓄積変異体の生理・生態特性の解析 (2011)
  • Author(s): 原田祐介
  • Organizer: 日本作物学会
  • Place of Presentation: 東京農業大学(神奈川県)
  • Year and Date: 2011-03-30