• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular mechanism of floral organogenesis using orchid model plant, Psygmorchis pusilla.

Research Project

Project/Area Number 22380018
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionTohoku University

Principal Investigator

KANNO Akira  東北大学, 大学院・生命科学研究科, 准教授 (10260449)

Co-Investigator(Kenkyū-buntansha) MII Masahiro  千葉大学, 大学院・園芸学研究科, 教授 (30093074)
HANDA Takashi  明治大学, 農学部, 教授 (00192708)
YUKAWA Tomohisa  独立行政法人国立科学博物館, 筑波実験植物園, 研究主幹 (50280524)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywordsシグモルキス / サギソウ / MADS-box遺伝子 / 遺伝子単離 / 遺伝子発現 / 形質転換 / 突然変異体 / アグロバクテリウム法 / ゲノム解析 / PLB培養
Research Abstract

In order to understand the molecular mechanism of the orchid-specific floral organs such as lip, column, pollinium, we established the cultivation system and Agrobacterium-mediated transformation system in Psygmorchis pusilla. We also analyzed the optimal condition for mutant generation with ion beam and EMS. cDNA fragment of a floral homeotic gene, DEFICIENS-like gene, was isolated from Psygmorchis pusilla and Habenaria radiata.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (31 results)

All 2013 2012 2011 2010

All Journal Article (9 results) (of which Peer Reviewed: 8 results) Presentation (14 results) Book (8 results)

  • [Journal Article] ランの花を遺伝子で語る-サギソウを例に2012

    • Author(s)
      菅野明
    • Journal Title

      Orchid

      Volume: 51巻 Pages: 28-31

    • Related Report
      2012 Final Research Report
  • [Journal Article] Identification of Dendrobium species used for herbal medicines based on ribosomal DNA internal transcribed spacer sequence2011

    • Author(s)
      Takamiya, T., T. Handa, T. Yukawa et al
    • Journal Title

      Biol. Pharm. Bull

      Volume: 34巻 Pages: 779-782

    • NAID

      130000657796

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of an APETALA1/FRUITFULL-like gene in Phalaenopsis sp. 'Hatsuyuki' (Orchidaceae). Hort2011

    • Author(s)
      Song, I.J., T. Fukuda, S.M. Ko, T. Ito, J. Yokoyama, H. Ichikawa, Y. Horikawa, T. Kameya, A. Kanno and H.Y. Lee
    • Journal Title

      Environ. Biotechnol

      Volume: 52巻 Pages: 183-195

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Dendrobium species used for herbal medicines based on ribosomal DNA internal transcribed spacer sequence2011

    • Author(s)
      Takamiya, T., T.Handa, T.Yukawa, et al
    • Journal Title

      Biol.Pharm.Bull.

      Volume: 34 Pages: 779-782

    • NAID

      130000657796

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of intraspecific hybrids between wild-type and petaloid-sepal cultivars in Habenaria radiata2010

    • Author(s)
      Kim, S.Y., M. Endo and A. Kanno
    • Journal Title

      Scientia Horticulturae

      Volume: 124巻 Pages: 415-418

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ectopic expression of an AP3-like and a PI-like genes from 'Sekkoku' orchid (Dendrobium moniliforme) causes the homeotic conversion of sepals to petals in whorl 1 and the suppression of carpel development in whorl 4 in Arabidopsis flowers2010

    • Author(s)
      Sirisawat, S., H. Ezura, N. Fukuda, T. Kounosu and T. Handa
    • Journal Title

      Plant Biotechnology

      Volume: 27巻 Pages: 183-192

    • NAID

      10026527239

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Az alea phylogeny reconstructed by means o f molecular techniques2010

    • Author(s)
      De Keyser, E., V. Scariot, N. Kobaya shi, J. De Riek and T. Handa
    • Journal Title

      Methods Mol. Bi ol

      Volume: 589巻 Pages: 349-364

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ectopic expression of an AP3-like and a PI-like genes from 'Sekkoku' orchid (Dendrobium moniliforme) causes the hom eotic conversion of sepals to petals in whorl 1 and the suppression of carpel development in whorl 4 in Arabidopsis flowers2010

    • Author(s)
      Sirisawat, S., H.Ezura, N.Fukuda, T.Kounosu, T.Handa
    • Journal Title

      Plant Biotechnology

      Volume: 27 Pages: 183-192

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Azalea phylogeny reconstructed by means of molecular techniques.2010

    • Author(s)
      De Keyser, E., V.Scariot, N.Kobayashi, J.De Risk, T.Handa
    • Journal Title

      Methods Mot.Biol.

      Volume: 589 Pages: 349-364

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Agrobacterium-mediated transformation of Psygmorchis pusilla2013

    • Author(s)
      Chin, D. P., Matsuda, H. and Mii, M
    • Organizer
      The 11th Asia Pacific Orchid Conference
    • Place of Presentation
      沖縄
    • Related Report
      2012 Final Research Report
  • [Presentation] Agrobacterium-mediated transformation of Psygmorchis pusilla.2013

    • Author(s)
      Chin, D. P., Matsuda, H. and Mii, M.
    • Organizer
      The 11th Asia Pacific Orchid Conference.
    • Place of Presentation
      沖縄
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effects of NAA and BA on PLB growth of Psygmorchis pusilla2012

    • Author(s)
      T. Kodama and T. Handa
    • Organizer
      International symposium on orchids and ornamental plants
    • Place of Presentation
      Chiang Mai (Thailand)
    • Year and Date
      2012-01-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Effects of NAA and BA on PLB growth of Psygmorchis pusilla2012

    • Author(s)
      T.Kodama, T.Handa
    • Organizer
      International symposium on orchids and ornamental plants
    • Place of Presentation
      ChiangMai (Thailand)
    • Year and Date
      2012-01-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] Expression analysis of paralogous DEFICIENS-like genes in the floral organs of Habenaria radiate (Orchidaceae)2012

    • Author(s)
      R. Hayashi, M. Endo, A. Kanno
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] Expression analysis of paralogous DEFICIENS-like genes in the floral organs of Habenaria radiata (Orchidaceae)2012

    • Author(s)
      R. Hayashi, M. Endo, A. Kanno
    • Organizer
      10th International Congress on Plant Molecular Biology
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラン科セッコク属Dendrobium節および近縁節の網羅的分子系統解析2011

    • Author(s)
      高宮知子・半田高・遊川知久, 他
    • Organizer
      第20回日本DNA多型学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-02
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] 石斛の基原植物(ラン科Dendrobium属)の遺伝子分類とHPLCプロファイルの相関に関する研究2011

    • Author(s)
      高宮知子・半田高・遊川知久, 他
    • Organizer
      日本生薬学会第58回年会
    • Place of Presentation
      昭和大学(東京)
    • Year and Date
      2011-09-24
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Presentation] ラン科Dendrobium属のrDNA-ITSデータベースを用いた生薬「石斛」の基源植物の同定2010

    • Author(s)
      高宮知子・Pheravut Wongsawad・田島奈津子・塩田奈緒・半田高・飯島洋・北中進・遊川知久
    • Organizer
      日本DNA多型学会
    • Place of Presentation
      三島市(静岡県)
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] SSRマーカーに基づく常緑性ツツジ九州野生集団の遺伝的構造の解明と園芸品種群との関係2010

    • Author(s)
      宮脇実桜・大澤良・半田高
    • Organizer
      園芸学会
    • Place of Presentation
      大分大学
    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Presentation] Morphological characteristics and genetic analysis of floral homeotic mutant in Habenaria radiata (Orchidaceae)2010

    • Author(s)
      Akira Kanno, So-Young Kim and Miyako Endo
    • Organizer
      国際園芸学会
    • Place of Presentation
      リスボ ン(ポルトガル)
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of microsatellite markers in Iris ensata (Iridaceae)2010

    • Author(s)
      Mori, T., C.Lian, R. Osawa, T. Tabuchi and T. Handa
    • Organizer
      国際園芸学会
    • Place of Presentation
      リスボン(ポルトガル)
    • Related Report
      2012 Final Research Report
  • [Presentation] Morphological characteristics and genetic analysis of floral homeotic mutant in Habenaria radiata (Orchidaceae).2010

    • Author(s)
      Akira Kanno, So-Young Kim, Miyako Endo
    • Organizer
      国際園芸学会
    • Place of Presentation
      リスボン(ポルトガル)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of microsatellite markers in Iris ensata (Iridaceae).2010

    • Author(s)
      Mori, T., C.Lian, R.Osawa, T.Tabuchi, T.Hands
    • Organizer
      国際園芸学会
    • Place of Presentation
      リスボン(ポルトガル)
    • Related Report
      2010 Annual Research Report
  • [Book] 東京,検定教科書「草花」2013

    • Author(s)
      半田高
    • Total Pages
      255
    • Publisher
      実教出版
    • Related Report
      2012 Final Research Report
  • [Book] 明治大学リバティーアカデミー,東京2013

    • Author(s)
      半田高
    • Total Pages
      46
    • Publisher
      ランの世界
    • Related Report
      2012 Final Research Report
  • [Book] 検定教科書「草花」2013

    • Author(s)
      半田 高
    • Total Pages
      255
    • Publisher
      実教出版,東京
    • Related Report
      2012 Annual Research Report
  • [Book] ランの世界2013

    • Author(s)
      半田 高
    • Total Pages
      46
    • Publisher
      明治大学リバティーアカデミー,東京
    • Related Report
      2012 Annual Research Report
  • [Book] Genetic transformation and analysis ofprotein-protein interaction of class B MADS-box genes from Dendrobium moniliforme. InTech, Rijeka2012

    • Author(s)
      Abdullakasim, S. and T. Handa
    • Publisher
      Current frontiers and perspectives in cell biology
    • Related Report
      2012 Final Research Report
  • [Book] Current frontiers and perspectives in cell biology2012

    • Author(s)
      Abdullakasim, S. and T. Handa
    • Total Pages
      556
    • Publisher
      InTech, Rijeka
    • Related Report
      2012 Annual Research Report
  • [Book] Protocols for in vitro propagation of ornamental plants2010

    • Author(s)
      Keyser, E., V. Scariot, N. Kobayashi and T. Handa and J. De Riek
    • Publisher
      Human Press, London
    • Related Report
      2012 Final Research Report
  • [Book] Protocols for in vitro propagation of ornamental plants2010

    • Author(s)
      Keyser, E., V.Scariot, N.Kobayashi, T.Hands, J.De Risk
    • Publisher
      Human Press, London
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi