| Project/Area Number |
22380023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Kyoto University |
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Principal Investigator |
YONEMORI Keizo 京都大学, (連合)農学研究科(研究院), 教授 (10111949)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Akihiko 農研機構果樹研究所, ブドウ・カキ研究 領域, 上級研究員 (30355440)
YAMANE Hisay0 京都大学, 大学院農学研究科, 講師 (80335306)
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| Co-Investigator(Renkei-kenkyūsha) |
KANZAKI Shinya 近畿大学, 農学部, 准教授 (20330243)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | 園芸学 / プロアントシアニジン / タンニン / ゲノム / 遺伝子 / 植物 |
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| Research Abstract |
Fosmid library was constructed by a diploid relative, Diospyros lotus, of D. kaki (hexaploid) for isolation of AST gene which confers astringency trait in persimmon. After screening seed clone from fosmid library by using a probe of AST-linked marker, fosmid contig was developed by further screening of the library using end-sequences of the clone. In addition, BAC library of D. lotus was constructed and BAC contig was also developed. Each contig seemed to contain AST locus there, so that we were approaching very near to isolate the AST gene. Furthermore, this BAC library was shown to be effective to isolate the CPCNA gene which confers the trait of non-astringency in Chinese-type non-astringent persimmon. Using the BAC library, isolation of CPCNA gene may also be possible.
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