Molecular characterization of sensitized house dust mite allergens for the generation of tailor-made vaccine
Project/Area Number |
22380190
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied molecular and cellular biology
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Research Institution | Hiroshima University |
Principal Investigator |
ONO Kazuhisa 広島大学, 大学院・先端物質科学研究科, 教授 (10144883)
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Co-Investigator(Kenkyū-buntansha) |
AKI Tsunehiro 広島大学, 大学院・先端物質科学研究科, 准教授 (80284165)
KAWAMOTO Seiji 広島大学, 大学院・先端物質科学研究科, 准教授 (90294537)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2010: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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Keywords | ダニアレルギー / 分子診断 / component-resolved diagnosis (CRD) / 感作アレルゲン / アレルギー / ダニ / component-resolved diagnosis / 感作抗原 / IgE反応性 |
Research Abstract |
Allergen-specific immunotherapy (SIT) is the only promising treatment of allergic disorders, but current SIT has problems such as the risk of side effects. Objective of this study is to construct a recombinant house dust mite allergen library, and to apply those allergen molecules for the development of molecular diagnostics and immunotherapeutics. Here, we showed a successful and comprehensive production of 23 major house dust mite allergens as a functional recombinant proteins. By using those recombinant allergen molecules, we also developed a molecular diagnostic system that enabled us to determine sensitized allergens among mite-allergic individuals.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Unexpected T cell regulatory activity of anti-histone H1 autoantibody: its mode of action in regulatory T cell-dependent and -independent manners2013
Author(s)
Takaoka Y. , Kawamoto S., Katayama A., Nakano T., Yamanaka Y., Takahashi M., Shimada Y., Chiang K. C., Ohmori N., Aki T., Goto T., Sato S., Goto S., Chen C. L., Ono K
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Journal Title
Biochem. Biophys. Res. Commun
Volume: 431
Pages: 246-252
Related Report
Peer Reviewed
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[Journal Article] Immunological and regenerative aspects of hepatic mast cells in liver allograft rejection and tolerance2012
Author(s)
Nakano T., Lai C. Y., Goto S., Hsu L. W., Kawamoto S., Ono K., Chen K. D., Lin C. C., Chiu K. W., Wang C. C., Cheng Y. F., Chen C. L
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Journal Title
NAID
Related Report
Peer Reviewed
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[Journal Article] Sake lees fermented with lactic acid bacteria prevents allergic rhinitis-like symptoms and IgE-mediated basophil degranulation2011
Author(s)
Kawamoto S., Kaneoke M., Ohkouchi K., Amano Y., Takaoka Y., Kume K., Aki T., Watanabe K., Kadowaki M., Hirata D., Ono K
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Journal Title
Biosci. Biotechnol. Biochem
Volume: 75
Pages: 140-144
Related Report
Peer Reviewed
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[Journal Article] Autoimmunity and liver transplantation immunology2011
Author(s)
Goto S., Nakano T., Hsu L. W., Chiang K. C., Lai C. Y., Kawamoto S., Ono K., Chen K. D., Ohmori N., Goto T., Sato S., Wang C. C., Jawan B., Cheng F. Y., Chen C. L
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Journal Title
Curr. Trends Immunol
Volume: 12
Pages: 1-11
Related Report
Peer Reviewed
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[Journal Article] Molecular cloning and immunochemical characterization of a novel major Japanese cedar pollen allergen belonging to the aspartic protease family2010
Author(s)
Ibrahim A. R. N., Kawamoto S., Aki T., Shimada Y., Rikimaru S., Onishi N., Babiker E. E., Oiso I., Hashimoto K., Hayashi T., Ono K
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Journal Title
Int. Arch. Allergy Immunol
Volume: 152
Pages: 207-218
Related Report
Peer Reviewed
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[Journal Article] Immunological aspects and therapeutic significance of an auto-Ab against histone H1 in a rat model of Con A-induced hepatitis2010
Author(s)
Nakano T., Goto S., Lai C. Y., Hsu L. W., Takaoka Y., Kawamoto S., Chiang K. C., Shimada Y., Ohmori N., Goto T., Sato S., Ono K., Cheng Y. F., Chen C. L
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Journal Title
Immunology
Volume: 129
Pages: 547-555
Related Report
Peer Reviewed
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[Journal Article] Selective detection of antigen-antibody reaction using Si ring optical resonators2010
Author(s)
Fukuyama M., Yamatogi S., Ding H., Nishida M., Kawamoto C., Amemiya Y., Ikeda T., Noda T., Kawamoto S., Ono K., Kuroda A., Yokoyama S
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Journal Title
Jpn. J. Appl. Phys
Volume: 49
NAID
Related Report
Peer Reviewed
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[Presentation] Component-resolved molecular diagnosis of house dust mite allergy using a comprehensive repertoire of recombinant allergens2013
Author(s)
Kishikawa Y., Kawamoto S., Nakahara H., Watakabe Y., Ota Y., Aki T., Asaoku Y., Hayashi T., Tanaka A., Ono K
Organizer
The EAACI-WAO Congress 2013
Place of Presentation
Milano, Italy (accepted for oral presentation)
Related Report
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[Presentation] Comprehensive expression of recombinant house dust mite allergens for component-resolved diagnosis2011
Author(s)
Watakabe Y., Kawamoto S., Nakahara H., Hiragun T., Aki T., Asaoku Y., Hayashi T., Mihara S., Hide M., Ono K
Organizer
The 22thWorld Allergy Congress (WAC 2011)
Place of Presentation
Cancun, Mexico
Related Report
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