Molecular dissection of the organization and dynamics of lipid domains in biomembranes
| Project/Area Number |
22390018
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biological pharmacy
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KOBAYASHI Toshihide 独立行政法人理化学研究所, 小林脂質生物学研究室, 主任研究員 (60162004)
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| Co-Investigator(Kenkyū-buntansha) |
ISHITSUKA Reiko 独立行政法人理化学研究所, 小林脂質生物学研究室, 専任研究員 (60342747)
ABE Mitsuhiro 独立行政法人理化学研究所, 小林脂質生物学研究室, 研究員 (90415068)
MURATE Motohide 独立行政法人理化学研究所, 小林脂質生物学研究室, 研究員 (30311369)
MAKINO Asami 独立行政法人理化学研究所, 小林脂質生物学研究室, 特別研究員 (20373368)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
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| Keywords | 脂質ドメイン / 脂質プローブ / 脂質ラフト / 脂質の非対称性 / スフィンゴミエリン / コレステロール / 脂質の可視化 / ホスファチジルイノシトール4,5-ビスリン酸 / 細胞分裂 / 超高解像顕微鏡 / スフィンゴ脂質 / 免疫電子顕微鏡 / X線散乱 |
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| Research Abstract |
We established a method to visualize major components of lipid rafts, sphingomyelin and cholesterol, in sub-micron scale.
New screening protocol using lipid imaging was employed to isolate a new inhibitor of sphingomyelin biosynthesis.
The role of sphingomyelin to regulate the transbilayer movement of lipid second messengers such as ceramide and diacylglycerol was elucidated.
The unusual stereoconfiguration of endosome-specific lipid was determined.
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Report
(4 results)
Research Products
(47 results)
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[Journal Article] Limonoid compounds inhibit sphingomyelin biosynthesis by preventing CERT protein-dependent extraction of ceramides from the endoplasmic reticulum2012
Author(s)
Hullin-Matsuda F, Tomishige N, Sakai S, Ishitsuka R, Ishii K, Makino A, Greimel P, Abe M, Laviad EL, Lagarde M, Vidal H, Saito T, Osada H, Hanada K, Futerman AH, and T. Kobayashi
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Journal Title
J Biol Chem
Volume: 287
Pages: 24397-24411
Related Report
Peer Reviewed
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[Journal Article] Phosphatidylglucoside forms specific lipid domains on the outer leaflet of the plasma membrane2010
Author(s)
Murate M, Hayakawa T, Ishii K, Inadome H, Greimel P, Watanabe Y, Nagatsuka Y, Ito K, Ito Y, Takahashi H, Hirabayashi Y, and T. Kobayashi
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Journal Title
Biochemistry
Volume: 49
Pages: 4732-4739
Related Report
Peer Reviewed
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[Journal Article] Marine antifungal theonellamides target 3β-hydroxysterol to activate Rho1 signaling2010
Author(s)
Nishimura S, Arita Y, Honda M, Iwamoto K, Matsuyama A, Shirai A, Kawasaki H, Kakeya H,Kobayashi T, Matsunaga S, and M. Yoshida
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Journal Title
Nat Chem Biol
Volume: 6
Pages: 519-526
Related Report
Peer Reviewed
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[Presentation] Imaging lipid domains Naito conference,2011
Author(s)
Kobayashi, T., Abe, M., Ishitsuka, R., Kishimoto, T., Makino, A., Hullin-Matsuda, F.,and M. Murate
Organizer
30^(th)Naito conference "Membrane Dynamics and Lipid Biology [II]"
Place of Presentation
Sapporo
Related Report
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