|Budget Amount *help
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Calorie restriction (CR) may exert anti-aging effects by modulating mitochondrial functions. We previously reported that CR upregulated mitochondrial proteins, including Cox6b1, a subunit of cytochrome c oxidase (Cox). To investigate the functional roles of Cox6b1 in mitochondria, we conducted in vitro experiments using Cox6b1-overexpressing cells or knockdown cells. The findings suggest that Cox6b1 mediates some of the effects, e.g., stress resistance, of CR by enhancing mitochondrial respiration via the assembly of Cox supercomplexes. The simultaneous generation of ROS may be prevented by several mechanisms, including Nrf2 activation. We also found that inhibition of glycogen synthase kinase (GSK)-3b, a putative upstream signaling molecule for Cox6b1, increased Cox6b1 protein expression. Accordingly, we confirmed that CR increased phosphorylated GSK-3b, its inactive form, in the mouse liver. The present study supports the mitohormesis hypothesis of CR.