Identification of cardiomyogenic factor in terms of cell-based therapy/regenerative medicine

• Project/Area Number: 22390072
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: UMEZAWA Akihiro 独立行政法人国立成育医療研究センター, 再生医療センター, センター長 (70213486)
• Co-Investigator(Kenkyū-buntansha): KURODA Masahiko 東京医科大学, 病理学教室, 教授 (80251304) ; TOYODA Masashi 地方独立行政法人東京都健康長寿医療センター, 研究所老年病研究チーム, 研究副部長 (50392486) ; 三好 俊一郎 慶應義塾大学, 医学部・循環器内科, 講師 (10296577)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 細胞・組織 / タンパク質 / 心筋再生 / 液性因子 / 再生医療 / 循環器 / ヒト幹細胞 / 人体バイオリソース / 心筋細胞 / 血管新生 / 心筋分化誘導因子 / 分化誘導

Outline of Final Research Achievements

We examined mesenchymal cells with an extended life span to investigate cardiomyogenic factors comprehensively. Among the mesenchymal cells, we focused on CD29-high CD34-low c-kit-positive CD140a-positive cells, and established a method to select cells capable of differentiating into cardiomyocytes in a high frequency. We implanted mesenchymal cells derived from a series of tissues including placenta into injured hearts. We herewith report to generate an experimental design to obtain proof of concept in a pre-clinical setting for cell-based therapy using tissue-derived mesenchymal cells to injured hearts.

Research Products

• [Journal Article] Stem Cells Bond Our Organs/Tissues and Engineering Products (2014)
  • Author(s): Toyoda M, Umezawa A.
  • Journal Title: Circulation Journal Volume: 78 Issue: 7 Pages: 1582-1583
  • DOI: 10.1253/circj.CJ-14-0558
  • NAID: 130004926975
  • ISSN: 1346-9843, 1347-4820
  • Peer Reviewed / Open Access
• [Journal Article] Pleiotropic functions of magnetic nanoparticles for ex vivo gene transfer. (2014)
  • Author(s): Kami D, Kitani T, Kishida T, Mazda O, Toyoda M, Tomitaka A, Ota S, Ishii R, Takemura Y, Watanabe M, Umezawa A, Gojo S.
  • Journal Title: Nanomedicine Volume: 10 Issue: 6 Pages: 1165-1174
  • DOI: 10.1016/j.nano.2014.03.018
  • Peer Reviewed / Open Access
• [Journal Article] Large-scale cell production of stem cells for clinical application using the automated cell processing machine. (2013)
  • Author(s): Kami D, Watakabe K, Yamazaki-Inoue M, Minami K, Kitani T, Itakura Y, Toyoda M, Sakurai T, Umezawa A, Gojo S.
  • Journal Title: BMC Biotechnol Volume: 13 Issue: 1 Pages: 102-102
  • DOI: 10.1186/1472-6750-13-102
  • Peer Reviewed
• [Journal Article] Allogeneic amniotic membrane-derived mesenchymal stromal cell transplantation in a porcine model of chronic myocardial ischemia. (2012)
  • Author(s): Kimura M, Toyoda M, Gojo S, Itakura Y, Kami D, Miyoshi S, Kyo S, Ono M, Umezawa A.
  • Journal Title: J. Stem Cells Regen. Med. Volume: 8 Pages: 171-180
  • Peer Reviewed