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Studies on Salmonella infection and host response using an accurate prediction system for screening effectors

Research Project

Project/Area Number 22390080
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionChiba University

Principal Investigator

YAMAMOTO Tomoko  千葉大学, 大学院・薬学研究院, 教授 (60110342)

Co-Investigator(Kenkyū-buntansha) TAKAYA Akiko  千葉大学, 大学院・薬学研究院, 准教授 (80334217)
SATO Yoshiharu  千葉大学, 大学院・薬学研究院, 助教 (00554586)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2010: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Keywordsサルモネラ / エフェクター / 宿主応答 / バイオインフォマティクス / 感染 / 自然免疫
Research Abstract

A broad range of pathogenic bacteria translocate a repertoire of effector proteins using the type 3 protein secretion system (T3SS) and enact the virulence program of the bacteria by directly interacting with host cell pathways. In this study, we have constructed an accurate prediction system for screening effectors on a genome-wide scale. Using this system, five candidates of novel effectors of Salmonella could be listed. Among them, GogA and GtgA2 were demonstrated to activate caspase-8 which has two opposing functions namely as an initiator of an apoptosis and in a non-apoptotic role including induction of the pro-inflammatory response. Another effector candidate, STM1239 was shown to be injected into macrophage cytoplasm by SPI1- and SPI2-T3SSs.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (66 results)

All 2013 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 10 results) Presentation (43 results) (of which Invited: 3 results) Book (5 results) Remarks (3 results)

  • [Journal Article] Studies on the molecular mechanism of Salmonella infection2013

    • Author(s)
      Yamamoto T
    • Journal Title

      Antibiotics Chemother

      Volume: 29 Pages: 473-483

    • Related Report
      2012 Final Research Report
  • [Journal Article] Regulation of Salmonella pathogenesis by effectors of Salmonella pathogenicity island 1 and 22013

    • Author(s)
      Takaya A
    • Journal Title

      Antibiotics Chemother

      Volume: 29 Pages: 62-71

    • Related Report
      2012 Final Research Report
  • [Journal Article] Studies on the molecular mechanism of Samonellainfection2013

    • Author(s)
      Yamamoto, T.
    • Journal Title

      Antibiotcs & Chemotherapy

      Volume: 29 Pages: 473-483

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Regulation of Salmonella pathogenesis by effectors of Salmonella pathogenicity island 1 and 22013

    • Author(s)
      Takaya A.
    • Journal Title

      Antibiotics & Chemotherapy

      Volume: 29 Pages: 62-71

    • Related Report
      2012 Annual Research Report
  • [Journal Article] YdiV: a dual function protein that targets FlhDC for ClpXP-dependent degradation by promoting release of DNA-bound FlhDC complex2012

    • Author(s)
      Takaya A, Erhardt M, Karata K, Winterberg K,Yamamoto T and Hughes K
    • Journal Title

      Mol.Microbiol

      Volume: 83 Pages: 1268-1284

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] YdiV: a dual function pttein that targets FlhDC for ClpXP-dependent degradation by promoting release of DNA-bound FlhDC complex.2012

    • Author(s)
      Takaya, A. et al
    • Journal Title

      Molecular Microbiology

      Volume: 83 Pages: 1268-1284

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞内寄生性細菌のストレス応答と病原性発現制御機構に関する研究2011

    • Author(s)
      山本友子
    • Journal Title

      日本細菌学雑誌

      Volume: 66 Pages: 517-529

    • NAID

      10030278685

    • Related Report
      2012 Final Research Report
  • [Journal Article] Meta-analytic approach to the accurate prediction of secreted virulence effectors in gram-negative bacteria2011

    • Author(s)
      Sato Y, Takaya A and Yamamoto T
    • Journal Title

      BMC Bioinformatics

      Volume: 12 Pages: 442-442

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dual regulatory pathways of flagellar gene expression by ClpXP protease in enterohaemorrhagic Escherichia coli2011

    • Author(s)
      Kitagawa R, Takaya A and Yamamoto T
    • Journal Title

      Microbiology

      Volume: 157 Pages: 3094-3103

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Meta-analytic approach to the accurate prediction of secreted virulence effectors in gram-negative bacteria2011

    • Author(s)
      Sato Y, Takaya A, Yamamoto T
    • Journal Title

      BMC Bioinformatics

      Volume: 12 Pages: 442-453

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual regulatory pathways of flagellar gene expression by ClpXP protease in enterohaemorrhagic Escherchia coli2011

    • Author(s)
      Kitagawa R, Takaya A, Yamamoto T
    • Journal Title

      Microbiology

      Volume: 157 Issue: 11 Pages: 3094-3103

    • DOI

      10.1099/mic.0.051151-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutational analysis of reduced telithromycin susceptibility of Streptococcus pneumoniae isolated clinically in Japan2010

    • Author(s)
      Takaya A, Kitagawa N, Kuroe Y, Endo K., Okazaki M, Yokoyama E, Wada A and Yamamoto T
    • Journal Title

      FEMS Microbiol. Lett

      Volume: 307 Pages: 87-93

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biogenesis of Salmonella enterica serovar Typhimurium membrane vesicles provoked by induction of PagC2010

    • Author(s)
      Kitagawa R, Takaya A, Ohya M, Mizunoe M, Takade A, Yoshida S, Isogai E and Yamamoto T
    • Journal Title

      J. Bacteriol

      Volume: 192 Pages: 5645-5656

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Biogenesis of Salmonella enteric serovar Typhimurium membrane vesicles provoked by induction of PagC2010

    • Author(s)
      Kitagawa R, Takaya A, Ohya M Mizunoe Y, Takade A, Yoshida S Isogai E, Yamamoto T
    • Journal Title

      Journal of Bacteriology

      Volume: 192 Pages: 5645-5656

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutational analysis of reduced telithromycin susceptibility of Streptococcus pneumoniae isolated clinically in Japan2010

    • Author(s)
      Takaya A, Kitagawa N, Kuroe Y, Endo K, Okazaki M, Yokoyama E, Wada A, Yamamoto T
    • Journal Title

      FEMS Microbiology Letter

      Volume: 307 Pages: 87-93

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Host-Salmonella Interaction: Control of inflammatory response and macrophage cell Ddeath by novel effectors2013

    • Author(s)
      Yamamoto, T.
    • Organizer
      第195回日本獣医学会学術集会
    • Place of Presentation
      東京大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] CspC/CspEによるサルモネラの感染初期マクロファージ殺菌回避機構2013

    • Author(s)
      前田侑也(山本友子)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2012 Annual Research Report
  • [Presentation] ClpXP プロテアーゼによるアダプタータンパク質を介したFlhD4C2認識・分解機構2013

    • Author(s)
      高屋明子(山本友子)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2012 Annual Research Report
  • [Presentation] 協調認識により調節されるAAA+プロテアーゼClpXPの基質特異性2013

    • Author(s)
      佐藤慶治(山本友子)
    • Organizer
      第86回日本細菌学会総会
    • Place of Presentation
      千葉
    • Related Report
      2012 Annual Research Report
  • [Presentation] Mechanism of telithromycin resistance in Streptococcus pneumoniae isolated clinically in Japan2012

    • Author(s)
      Shibata T , Takaya A, Sato Y, Yamamoto T
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] A study on the Salmonella secreted protein STM1670 identified by machine learning approach2012

    • Author(s)
      Sato Y, Takaya A, Yamamoto T
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] The antibiotic mechanism of telithromycin in Streptococcus pneumoniae2012

    • Author(s)
      Takaya A, Sato Y, Yamamoto T
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2012-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Bioinformatics-assisted identification of effectors based on feature analysis of secreted signal2012

    • Author(s)
      Sato Y, Takaya A, Yamamoto T
    • Organizer
      第85回日本細菌学会総会
    • Place of Presentation
      長崎
    • Year and Date
      2012-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] The SPI1-flagellar regulatory circuit of Salmonella: negative regulation by YdiV, a dual-function protein induced under nutrient starvation2012

    • Author(s)
      Yamamoto T
    • Organizer
      47^<th> Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections Chiba
    • Related Report
      2012 Final Research Report
  • [Presentation] Inactivation of RlmA^<II> confers the telithromycin-resistance to Streptococcus pneumoniae2012

    • Author(s)
      Takaya A, Sato Y, Shouji T, Yamamoto T
    • Organizer
      II International Conference on Antimicrobial Research
    • Place of Presentation
      Lisbon ,Portugal
    • Related Report
      2012 Final Research Report
  • [Presentation] 山本友子サルモネラYdiVの2つの機能によるべん毛マスターレギュレーターFlhD_4C_2活性制御2012

    • Author(s)
      高屋明子,佐藤慶冶
    • Organizer
      第95回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] サルモネラによるマクロファージ細胞死誘導とその制御2012

    • Author(s)
      山本友子
    • Organizer
      第79回日本細菌学会北海道支部総会
    • Place of Presentation
      帯広
    • Related Report
      2012 Final Research Report
  • [Presentation] Control of Salmonella pathogenicity island expression on the stress-induced regulatory loop within macrophages2012

    • Author(s)
      Yamamoto T
    • Organizer
      Rudbeck Life Science Symposium
    • Place of Presentation
      Uppsala, Sweden
    • Related Report
      2012 Final Research Report
  • [Presentation] Control of inflammatory response and macrophage cell death by Salmonella2012

    • Author(s)
      Takaya A
    • Organizer
      Rudbeck Life Science Symposium
    • Place of Presentation
      Uppsala, Sweden
    • Related Report
      2012 Final Research Report
  • [Presentation] The SPI1-flagellar regulatory circuit of Salmonella: negaive regulation by YdiV, a dual-function protein induced under nutrient starvation2012

    • Author(s)
      Yamamoto, T.
    • Organizer
      47th Annual Joint Panel Meeting on Cholera and Other Bacterial Enteric Infections
    • Place of Presentation
      Chiba University
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] サルモネラによるマクロファージ細胞死誘導とその制御2012

    • Author(s)
      山本友子
    • Organizer
      第79回日本細菌学会北海道支部学術総会
    • Place of Presentation
      帯広畜産大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Inactivation of RlmAII confers the telithromycin-resistance to Streptococcus pneumoniae2012

    • Author(s)
      Takaya, A. (Yamamoto, T)
    • Organizer
      II International Conference on Antimicrobial Reserach
    • Place of Presentation
      Lisbon、Portugal
    • Related Report
      2012 Annual Research Report
  • [Presentation] An integrated bioinformatics approach for the identification of novel effectors in Salmonella2011

    • Author(s)
      Sato Y, Takaya A, Yamamoto T
    • Organizer
      46^<th> Conference of US-Japan Cooperative Medical Science Program, Kolkata
    • Place of Presentation
      India
    • Year and Date
      2011-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] An integrated bioinformatics approach for the identification of novel effectors in Salmonella2011

    • Author(s)
      佐藤慶冶
    • Organizer
      46^<th> Joint conference on cholera and other bacterialenteric infections panel
    • Place of Presentation
      Kolkata(招待講演)
    • Year and Date
      2011-12-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細菌が病原性をコントロールするしくみ2011

    • Author(s)
      山本友子
    • Organizer
      微生物研究会
    • Place of Presentation
      松戸
    • Year and Date
      2011-11-12
    • Related Report
      2012 Final Research Report
  • [Presentation] YdiV is a novel adaptor protein for ClpXP protease2011

    • Author(s)
      Takaya A, Sato Y, Yamamoto T
    • Organizer
      9^<th> International Conference on AAA Proteins
    • Place of Presentation
      Kumamoto
    • Year and Date
      2011-11-08
    • Related Report
      2012 Final Research Report
  • [Presentation] YdiV is a novel adaptor protein for ClpXP protease2011

    • Author(s)
      高屋明子
    • Organizer
      9^<th> International conference on AAA proteins
    • Place of Presentation
      Kumamoto(招待講演)
    • Year and Date
      2011-11-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Bacterial strategies hijacking host cell functions for a successful function2011

    • Author(s)
      Yamamoto T
    • Organizer
      Joint symposium of Seoul National University and Chiba University
    • Place of Presentation
      Seoul, Korea
    • Year and Date
      2011-10-14
    • Related Report
      2012 Final Research Report
  • [Presentation] Telithromycin-resistant Streptococcus pneumoniae clinically isolated between 2009 and 2010 in Japan2011

    • Author(s)
      Ito M, Shibata T, Takaya A, Sato Y, Endo K, Yamamoto T
    • Organizer
      IUMS Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Combination of erm gene and mutation in riboproteins results in high-level telithromycin resistance in Streptococcus pneumoniae2011

    • Author(s)
      Sone K, Takaya A, Sato Y, Endo K, Yamamoto T
    • Organizer
      IUMS Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内寄生性細菌のストレス応答と病原性発現制御機構に関する研究2011

    • Author(s)
      山本友子
    • Organizer
      第84回日本細菌学会総会
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内規制性細菌のストレス応答と病原性発現制御機構に関する研究2011

    • Author(s)
      山本友子
    • Organizer
      第84回日本細菌学会総会
    • Place of Presentation
      札幌(招待講演)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Novel Salmonella effectors capable of activating caspase-8 in macrophages, leading to induction of a pro-inflammatory response2011

    • Author(s)
      Yamamoto T
    • Organizer
      IUMS Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Meta-analysis based prediction system of secreted virulence effectors2011

    • Author(s)
      Sato Y, Takaya A, Yamamoto T
    • Organizer
      IUMS
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of novel Salmonella effectors by genome-wide in silico screening2011

    • Author(s)
      Samizo C, Sato Y, Takaya A, Yamamoto T
    • Organizer
      IUMS Congress
    • Place of Presentation
      Sapporo
    • Year and Date
      2011-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Salmonella effectors capable of activating caspase-8 in macrophage cells, leading to induction of a pro-inflammatory response2011

    • Author(s)
      山本友子
    • Organizer
      IUMS2011
    • Place of Presentation
      Sapporo(招待講演)
    • Year and Date
      2011-09-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] 山本友子肺炎球菌のテリスロマイシン高度耐性化機構2011

    • Author(s)
      高屋明子,曽根佳央里,佐藤慶冶
    • Organizer
      第94回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Salmonella effectors, GogA and GtgA2 are involved in induction of pro-inflammatory response within murine bone marrow-derived macrophage cells2011

    • Author(s)
      Takaya A, Sato Y, Yamamoto T
    • Organizer
      IUMS Congress, Sapporo
    • Place of Presentation
      山本友 子細菌が病原性をコントロー
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel Salmonella effectors capable of activating caspase-8 in macrophages2010

    • Author(s)
      Yamamoto T
    • Organizer
      45^<th> Conference of US-Japan CooperativeMedical Science Program
    • Place of Presentation
      Kyoto
    • Year and Date
      2010-12-07
    • Related Report
      2012 Final Research Report
  • [Presentation] サルモネラ感染におけるマクロファージCaspase-8活性化による細胞死制御2010

    • Author(s)
      原貴史,高屋明子,山本友子
    • Organizer
      第93回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-21
    • Related Report
      2012 Final Research Report
  • [Presentation] サルモネラSPI1転写制御因子HilDによるべん毛レギュロン制御機構2010

    • Author(s)
      谷川育己,高屋明子,山本友子
    • Organizer
      第93回日本細菌学会関東支部総会
    • Place of Presentation
      東京
    • Year and Date
      2010-10-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Host-Salmonella Interaction: Control of inflammatory response and host cell death by Salmonella Pathogenicity Island 12010

    • Author(s)
      Yamamoto T
    • Organizer
      The 5^<th> Asian Congress of Pediatric Infectious Diseases, Taiwan
    • Place of Presentation
      China
    • Year and Date
      2010-09-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Host-Salmonella interaction : Control of inflammatory response and host cell death by Salmonella Pathogenicity Island 12010

    • Author(s)
      Yamamoto T
    • Organizer
      The 5^<th>Asian Congress of Pediatric Infectious Diseases
    • Place of Presentation
      Taipei, Taiwan
    • Year and Date
      2010-09-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] Bacterial strategies for hijacking of host cells to cause systemic disease2010

    • Author(s)
      Yamamoto T
    • Organizer
      The 10^<th> Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Awaji
    • Year and Date
      2010-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Acceleration of membrane vesicle release by PagC in Salmonella2010

    • Author(s)
      Kitagawa R, Takaya A, Yamamoto T
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Novel posttranscriptional control mechanism of flagellar regulator2010

    • Author(s)
      Fujinawa M, Takaya A, Yamamoto T
    • Organizer
      第33回日本分子
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular analysis of reduced telithromycin susceptibility of Streptococcus pneumoniae collected clinically between 2005 and 2009 in Japan2010

    • Author(s)
      Takaya A, Yamamoto T
    • Organizer
      I International Conference on Antimicrobial Research. Valladolid
    • Place of Presentation
      Spain
    • Related Report
      2012 Final Research Report
  • [Presentation] Bioinformatics-assisted identification of novel virulence effectors in Salmonella2010

    • Author(s)
      Sato Y, Takaya A, Yamamoto T
    • Organizer
      The 10^<th> Awaji International Forum on Infection and Immunity
    • Place of Presentation
      Awaji
    • Related Report
      2012 Final Research Report
  • [Book] 薬科微生物学第6版2013

    • Author(s)
      加藤文男編
    • Publisher
      丸善
    • Related Report
      2012 Final Research Report
  • [Book] 薬科微生物学第6版2013

    • Author(s)
      加藤文男
    • Total Pages
      333
    • Publisher
      丸善
    • Related Report
      2012 Annual Research Report
  • [Book] 微生物学-基礎から臨床へのアプローチ2012

    • Author(s)
      神谷茂編
    • Publisher
      メディカルサイエンスインターナショナル
    • Related Report
      2012 Final Research Report
  • [Book] 微生物学ー基礎から臨床へのアプローチ2012

    • Author(s)
      神谷茂
    • Total Pages
      664
    • Publisher
      メディカル・サイエンス・インターナショナル
    • Related Report
      2012 Annual Research Report
  • [Book] 薬科微生物学2010

    • Author(s)
      加藤文男編
    • Total Pages
      318
    • Publisher
      丸善株式会社
    • Related Report
      2010 Annual Research Report
  • [Remarks] 千葉大学大学院薬学研究院微生物薬品化学研究室

    • URL

      http://www.p.chiba-u.ac.jp/lab/bisei/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.p.chiba-u.ac.jp/lab/bisei/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.p.chiba-u.ac.jp/lab/bisei/index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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