Identification and analysis of regulatory sRNAs involved inthe pathogenicity of gas-gangrene related bacteria

• Project/Area Number: 22390081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Kanazawa University
• Principal Investigator: SHIMIZU Tohru 金沢大学, 医学系, 教授 (80235655)
• Co-Investigator(Renkei-kenkyūsha): OHTANI Kaori 金沢大学, 医学系, 講師 (30377410)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: ウェルシュ菌 / 調節RNA分子 / ガス壊疽 / 食中毒 / 芽胞(胞子) / 転写調節RNA / ウェルシュ菌食中毒 / 芽胞 / 病原性 / 遺伝子発現調節 / 調節RNA / 遺伝子発現調

Research Abstract

Clostridium perfringens is a causative agent for gas gangrene and controls toxin production via complex regulatory networks. In this study, we identified many novel sRNAs in C. perfringens and showed that some of the sRNAs are also regulated by a major regulatory system for toxin production. Furthermore, we found that one of the sRNAs, virX is involved in the regulation of spore formation which turns into production of enterotoxin for food poisoning.

Research Products

• [Journal Article] Unique regulatory mechanism of sporulation and enterotoxin production in Clostridium perfringens (2013)
  • Author(s): Ohtani, K.
  • Journal Title: Journal of Bacteriology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Complex transcriptional regulation of citrate metabolism in Clostridium perfringens (2012)
  • Author(s): Yuan, Y., Ohtani, K., Yoshizawa, S., Shimizu, T
  • Journal Title: Anaerobe Volume: 18 Pages: 48-54
  • DOI: 10.1016/j.anaerobe.2011.09.004
  • Peer Reviewed
• [Journal Article] Global regulation of gene expression in response to cysteine availability in Clostridium perfringens (2010)
  • Author(s): Andre, G., Haudecoeur, E., Monot, M., Ohtani, K., Shimizu, T.,(他)
  • Journal Title: BMC Microbiol Volume: 10 Pages: 234-234
  • DOI: 10.1186/1471-2180-10-234
  • Peer Reviewed
• [Journal Article] Identification of a two-component VirR/Vir S regulon in Clostridium perfringens (2010)
  • Author(s): Ohtani, K., Hirakawa, H., Tashiro, K., Yoshizawa, S., Kuhara, S., Shimizu, T
  • Journal Title: Anaerobe Volume: 16 Pages: 258-264
  • DOI: 10.1016/j.anaerobe.2009.10.003
  • Peer Reviewed
• [Journal Article] Identification of a two-component VirR/VirS regulon in Clostridium perfringens (2010)
  • Author(s): Ohtani, et.al.
  • Journal Title: Anaerobe Volume: 16 Pages: 258-264
  • Peer Reviewed
• [Journal Article] Global regulation of gene expression in response to cysteine availability in Clostridium perfrineens (2010)
  • Author(s): Andre, et. al.
  • Journal Title: BMC Microbiol Volume: 10 Pages: 234-240
  • Peer Reviewed
• [Journal Article] Unique regulatory mechanism of sporulation and enterotoxin production in Clostridium perfringens
  • Author(s): Ohtani, K., Hirakawa, H., Paredes-Sabja, D., Tashiro, K., Kuhara, S., Sarker, M., Shimizu, T
  • Journal Title: J. Bacteriol
  • Peer Reviewed
• [Presentation] Novel regulatory mechanism for spore formation and enterotoxin production in Clostridium perfringens (2013)
  • Author(s): 大谷 郁, Huyen Hoang, 清水 徹
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 幕張メッセ国際会議場(千葉県)
  • Year and Date: 2013-03-18
• [Presentation] ウェルシュ菌における芽胞形成およびエンテロトキシン産生調節機構の解析 (2013)
  • Author(s): 大谷 郁
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉県千葉市幕張メッセ国際会議場
• [Presentation] Subtilase cytotoxinはPERK依存的経路を介してストレス顆粒の形成を誘導する (2013)
  • Author(s): 津々木博康
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉県千葉市幕張メッセ国際会議場
• [Presentation] Novel regulatory mechanism for spore formation and enterotoxin production in Clostridium perfringens (2011)
  • Author(s): Ohtani, K, et al.
  • Organizer: 7th International Conference of the Molecular Biology and Pathogenesis of the Clostridia
  • Place of Presentation: アメリカ・アイオワ大学(招待講演)
  • Year and Date: 2011-10-27
• [Presentation] Nove; regulatory mechanism for sopre formation and enterotoxin production in Clostridium perfringens (2011)
  • Author(s): Ohtani, K., Hirakawa, H., Paredes-Sabja, D., Tashiro, K., Kuhara, S., Sarker, M., Shimizu, T
  • Organizer: 7th International Conference on the Molecular Biology and Pathogenesis o the Clostridia
  • Place of Presentation: Iowa State Center (Ames, Iowa, USA)