RNA Biology for Stress Assessment and Prediction of Major Depression

• Project/Area Number: 22390146
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: The University of Tokushima
• Principal Investigator: ROKUTAN Kazuhito 徳島大学, 大学院・へルスバイオサイエンス研究部, 教授 (10230898)
• Co-Investigator(Kenkyū-buntansha): KUWANO Yuki 徳島大学, 大学院・へルスバイオサイエンス研究部, 助教 (00563454)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: ストレス / うつ病 / 遺伝子発現 / RNAマーカー / マイクロRNA

Research Abstract

This research project was designed to identify novel RNA markers for stress assessment and prediction of major depressive disorders. We reported acute stress-specific alternative splicing of SMG-1in peripheral blood (Neurosci Lett 2010) and chronic stress-specific reduction of a GR-beta isoform (Stress 2011). We also investigated stress-responsive microRNAs (miRNAs) in peripheral blood. We reported miR-16, miR-144, and miR144* as acute stress-responsive miRNAs (nuerosci Lett 2012).We also identified 7 miRNAs as chronic stress-responsive miRNAs.

Research Products

• [Journal Article] Down-regulation of serine/arginine-rich splicing factor 3 induces G1 cell cycle arrest and apoptosis in colon cancer cells (2014)
  • Author(s): Kurokawa K, Akaike Y, Masuda K, Kuwano Y, Nishida K, Yamagishi N, Kajita K, Tanahashi T, Rokutan K.
  • Journal Title: J Gastroenterol. Volume: 33 Pages: 1407-1417
  • DOI: 10.1038/onc.2013.86
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Downregulation of serine/arginine-rich splicing factor 3 induces G1 cell cycle arrest and apoptosis in colon cancer cells (2013)
  • Author(s): Kurokawa K, Akaike Y, Masuda K, Kuwano Y, Nishida K, Yamagishi N, Kajita K, Tanahashi T, Rokutan K
  • Journal Title: Oncogene Volume: 86
• [Journal Article] Chronic inhibition of tumor cell-derived VEGF enhances the malignant phenotype of colorectal cancer cells (2013)
  • Author(s): Yamagishi N, Teshima-Kondo S, Masuda K, Nishida K, Kuwano Y, Dang DT, Dang LH, Nikawa T, Rokutan K.
  • Journal Title: BMC Cancer. Volume: 13 Pages: 1-11
  • DOI: 10.1186/1471-2407-13-229
  • Peer Reviewed
• [Journal Article] Ets1 and heat shock factor 1 regulate transcription of the Transformer 2beta gene in human colon cancer cell (2013)
  • Author(s): Kajita K, Kuwano Y, Kitamura N, Satake Y, Nishida K, Kurokawa K, Akaike Y, Honda M, Masuda K, Rokutan K
  • Journal Title: J Gastroenterol
  • URL: http://link.springer.com/article/10.1007%2Fs00535-012-0745-2
• [Journal Article] Molecular features of triple negative breast cancers by genome-wide gene expression profiling analysis (2013)
  • Author(s): Komatsu M, Yoshimaru T, Matsuo T, Kiyotani K, Miyoshi Y, Tanahashi T, Rokutan K, Yamaguchi R, Saito A, Imoto S, Miyano S, Nakamura Y, Sasa M, Shimada M, Katagiri T.
  • Journal Title: Int J Oncol Volume: 42(2) Pages: 478-506
  • DOI: 10.3892/ijo.2012.1744
  • Peer Reviewed
• [Journal Article] Associations between chronotype and salivary endocrinological stress markers. (2013)
  • Author(s): Toda M, Kawai T, Takeo K, Rokutan K, Morimoto K
  • Journal Title: Endocr Res. Volume: 38 Pages: 1-7
  • DOI: 10.3109/07435800.2012.683225
  • Peer Reviewed
• [Journal Article] Circulating cytokine signatures in healthy medical students exposed to academic examination stress (2013)
  • Author(s): Kan C, Kawakami N, Karasawa M, Love GD, Coe CL, Miyamoto Y, Ryff CD, Kitayama S, Curhan KB, Markus HR.
  • Journal Title: Psychophysiology Volume: 49(7) Pages: 991-7
  • DOI: 10.1111/j.1469-8986.2012.01371.x
  • Peer Reviewed
• [Journal Article] Ets1 and heat shock factor 1 regulate transcription of the Transformer 2beta gene in human colon cancer cells (2013)
  • Author(s): Kajita K
  • Journal Title: Journal of Gastroenterology Volume: in press
  • Peer Reviewed
• [Journal Article] MicroRNAs miR-144/ 144* and miR-16 in peripheral blood are potential biomarkers for naturalistic stress in healthy Japanese medical students (2012)
  • Author(s): Katsuura S, Kuwano Y, Yamagishi N, Kurokawa K, Kajita K, Akaike Y, Nishida K, Masuda K, Tanahashi T, Rokutan K.
  • Journal Title: Neuroscience Letters Volume: 516 Pages: 79-84
  • DOI: 10.1016/j.neulet.2012.03.062
  • Peer Reviewed
• [Journal Article] Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells. (2012)
  • Author(s): Kurokawa K, Tanahashi T, Iima T, Yamamoto Y, Akaike Y, Nishida K, Masuda K, Kuwano Y, Murakami Y, Fukushima M, Rokutan
  • Journal Title: J Gastroenterol. Volume: 47 Pages: 883-895
  • DOI: 10.1007/s00535-012-0547-6
  • NAID: 10031051201
  • Peer Reviewed
• [Journal Article] General RBP expression in human tissues as a function of age (2012)
  • Author(s): Masuda K, et al.
  • Journal Title: Ageing research reviews Volume: 11(4) Pages: 423-31
  • DOI: 10.1016/j.arr.2012.01.005
  • Peer Reviewed
• [Journal Article] Circulating vascular endothelial growth factor is independently and negatively associated with trait anxiety and depressive mood in healthy Japanese university students (2012)
  • Author(s): Katsuura S, Kamezaki Y, Yamagishi N, Kuwano Y, Nishida K, Masuda K, Tanahashi T, Kawai T, Arisawa K, Rokutan K.
  • Journal Title: Int J Psychophysio Volume: 81 Pages: 38-43
  • DOI: 10.1016/j.ijpsycho.2011.04.004
  • Peer Reviewed
• [Journal Article] Circulating cytokine signatures in healthy medical students exposed to academic examination stress (2012)
  • Author(s): Kamezaki Y, et al
  • Journal Title: Psychophysiology Volume: (In press)
  • Peer Reviewed
• [Journal Article] Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells (2012)
  • Author(s): Kurokawa K, et al
  • Journal Title: Journal of Gastroenterology Volume: (In press)
  • Peer Reviewed
• [Journal Article] General RBP expression in human tissues as a function of age (2012)
  • Author(s): Masuda K, et al
  • Journal Title: Aging Research Review Volume: (In press)
  • Peer Reviewed
• [Journal Article] Effects of chronic academic stress on mental state and expression of glucocorticoid receptor alpha and beta isoforms in healthy Japanese medical students (2011)
  • Author(s): Kurokawa K, Tanahashi T, Murata A, Akaike Y, Katsuura S, Nishida K, Masuda K, Kuwano Y, Kawai T, Rokutan K.
  • Journal Title: Stress Volume: 14 Pages: 431-438
  • DOI: 10.3109/10253890.2011.555930
  • Peer Reviewed
• [Journal Article] Autism-associated gene expression in peripheral leucocytes commonly observed between subjects with autism and healthy women having autism children (2011)
  • Author(s): Kuwano Y, Kamio Y, Kawai T, Katsuura S, Inada N, Takaki A, Rokutan K.
  • Journal Title: PloS ONE Volume: 6
  • DOI: 10.1371/journal.pone.0024723
  • Peer Reviewed
• [Journal Article] Skipping of an alternative intron in the srsf1 3' untranslated region increases transcript stabilit (2011)
  • Author(s): Akaike Y, Kurokawa K, Kajita K, Kuwano Y, Masuda K, Nishida K, Wan Kang S, Tanahashi T, Rokutan K
  • Journal Title: J Med Invest Volume: 58 Pages: 180-187
  • URL: https://www.jstage.jst.go.jp/article/jmi/58/3,4/58_3,4_180/_article
  • Peer Reviewed
• [Journal Article] Autism-associated gene expression in peripheral leucocytes commonly observed between subjects with autism and healthy women having autistic children (2011)
  • Author(s): Kuwano Y, et al
  • Journal Title: PLoS One Volume: 6
  • Peer Reviewed
• [Journal Article] Skipping of an alternative intron in the srsfl 3' untranslated region increases transcript stability (2011)
  • Author(s): Akaike Y, et al
  • Journal Title: Journal of Medical Investigation Volume: 58 Pages: 180-187
  • Peer Reviewed
• [Journal Article] Circulating vascular endothelial growth factor is independently and negatively associated with trait anxiety and depressive mood in healthy Japanese university students (2011)
  • Author(s): Katsuura S, et al
  • Journal Title: International Journal of Psychophysiology Volume: 81 Pages: 38-43
  • Peer Reviewed
• [Journal Article] Effects of chronic academic stress on mental state and expression of glucocorticoid receptor α and β isoforms in healthy Japanese medical students (2011)
  • Author(s): Kurokawa K, et al
  • Journal Title: Stress Volume: 14 Pages: 431-438
  • Peer Reviewed
• [Journal Article] Effects of chronic academic stress on mental state an expression of glucocorticoid receptor α and β isoforms in healthy Japanese medical students (2011)
  • Author(s): Ken Kurokawa, et al.
  • Journal Title: Stress Volume: (in press)
  • Peer Reviewed
• [Journal Article] Interleukin-18 deficiency reduces neuropeptide gene expressions in the mouse amygdala related with behavioral change (2010)
  • Author(s): Yamamoto Y, Tanahashi T, Katsuura S, Kurokawa K, Nishida K, Kuwano Y, Kawai T, Teshima-Kondo S, Chikahisa S, Tsuruo Y, Sei H, Rokutan K.
  • Journal Title: J Neuroimmunol Volume: 229 Pages: 129-139
  • DOI: 10.1016/j.jneuroim.2010.07.024
  • Peer Reviewed
• [Journal Article] Brief naturalistic stress induces an alternative splice variant of SMG-1 lacking exon 63 in peripheral leukocytes (2010)
  • Author(s): Kurokawa K, Kuwano Y, Tominaga K, Kawai T, Katsuura S, Yamagishi N, Satake Y, Kajita K, Tanahashi T, Rokutan K
  • Journal Title: Neurosci Lett Volume: 484 Pages: 128-132
  • DOI: 10.1016/j.neulet.2010.08.031
  • Peer Reviewed
• [Journal Article] High-throughput screening of brief naturalistic stress-responsive cytokines in university students taking examinations (2010)
  • Author(s): Katsuura S, Kamezaki Y, Tominaga K, Masuda K, Nishida K, Yamamoto Y, Takeo K, Yamagishi N, Tanahashi T, Kawai T, Rokutan K
  • Journal Title: Int J Psychophysiol Volume: 77 Pages: 135-140
  • DOI: 10.1016/j.ijpsycho.2010.05.004
  • Peer Reviewed
• [Journal Article] Brief naturalistic stress induces an alternative splice variant of SMG-1 lacking exon 63 in peripheral leukocytes (2010)
  • Author(s): Ken Kurokawa, et al.
  • Journal Title: Neuroscience Letters Volume: 484 Pages: 128-132
  • Peer Reviewed
• [Journal Article] Interleukin-18 deficiency reduces neuropeptide gene expressions in the mouse amygdala related with behavioral change (2010)
  • Author(s): Yuta Yamamoto, et al.
  • Journal Title: Journal of Neuroimmunology Volume: 229 Pages: 129-139
  • Peer Reviewed
• [Journal Article] High-throughput screening of brief naturalistic stress-responsive cytokines in university students taking examinations (2010)
  • Author(s): Sakurako Katsuura, et al.
  • Journal Title: International Journal of Psychophysiology Volume: 77 Pages: 135-140
  • Peer Reviewed
• [Presentation] 遺伝子発現解析からみた慢性疲労症候群の病態 (2011)
  • Author(s): 六反一仁
  • Organizer: 第7回日本疲労学会総会
  • Place of Presentation: 名古屋大学(名古屋市)(招待講演)
  • Year and Date: 2011-05-21
• [Presentation] ストレスマーカーの現状 (2010)
  • Author(s): 六反一仁
  • Organizer: 第26回日本ストレス学会学術総会教育講演
  • Place of Presentation: 福岡市
  • Year and Date: 2010-11-06
• [Presentation] 心と遺伝子を探るストレスゲノミクス研究 (2010)
  • Author(s): 六反一仁
  • Organizer: 2010日本歯周痛学会秋期学術大会(第53回)
  • Place of Presentation: 高松市(特別講演)
  • Year and Date: 2010-09-19
• [Presentation] Alternative splice variants of SMG-1 as a potential marker for brief naturalistic stressors peripheral leukocytes (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden, Netherlands
• [Presentation] High-throughput screening of brief naturalistic stress-responsive cytokines in Japanese university students taking examinations (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden, Netherlands
• [Presentation] High-throughput screening of immunomodulators identifies VEGF as a potential biomarker for trait anxiety and depressive mood in healthy Japanese university students (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: The 7^<th> World Congress on Stress
  • Place of Presentation: Leiden, Netherlands
• [Presentation] The mechanism of transformer 2-□ gene expression in response to oxidative stress in human colon epithelial cells (2010)
  • Author(s): Kazuhito Rokutan
  • Organizer: Digestive Disease Week 2010
  • Place of Presentation: New Orleans, USA
• [Remarks]
  • URL: http://www.tokushima-u.ac.jp/med/culture/sutoresu/